Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
RecombinantRabMAb

Recombinant Anti-NeuN antibody [EPR21902] (ab236869)

Submit a review Submit a question
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR21902] (ab236869)
  • Western blot - Anti-NeuN antibody [EPR21902] (ab236869)
  • Immunoprecipitation - Anti-NeuN antibody [EPR21902] (ab236869)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR21902] (ab236869)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR21902] (ab236869)
  • Anti-NeuN antibody [EPR21902] (ab236869)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21902] to NeuN
  • Suitable for: IHC-P, WB, IP
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-NeuN antibody [EPR21902]
    See all NeuN primary antibodies

  • Description

    Rabbit monoclonal [EPR21902] to NeuN

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, IPmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human fetal brain, brain, cerebellum and hippocampus lysate. IHC-P: Human retina, cerebellum and cerebrum tissues. IP: Human fetal brain lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab236869 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/1000. Detects a band of approximately 46, 48 kDa (predicted molecular weight: 34 kDa).
IP
1/30.
Notes
IHC-P
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/1000. Detects a band of approximately 46, 48 kDa (predicted molecular weight: 34 kDa).
IP
1/30.

Target

  • Function

    RNA-binding protein that regulates alternative splicing events.

  • Sequence similarities

    Contains 1 RRM (RNA recognition motif) domain.

  • Cellular localization

    Nucleus. Cytoplasm.
  • Information by UniProt

  • Database links

    • Alternative names

      • FLJ56884 antibody
      • FLJ58356 antibody
      • Fox-1 homolog C antibody
      • fox1 homolog C antibody
      • Fox3 antibody
      • FOX3NeuN antibody
      • hexaribonucleotide binding protein 3 antibody
      • HRNBP3 antibody
      • NEUN antibody
      • neuronal nuclei antibody
      • Rbfox3 antibody
      • RFOX3_HUMAN antibody
      • RNA binding protein fox-1 homolog 3 antibody
      • RNA binding protein, fox 1 homolog (C. elegans) 3 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR21902] (ab236869)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR21902] (ab236869)

      Immunohistochemical analysis of paraffin-embedded human retina tissue labeling NeuN with ab236869 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear and cytoplasmic staining in ganglion cells of human retina (PMID:8813082; PMID:1483388). Counterstained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Western blot - Anti-NeuN antibody [EPR21902] (ab236869)
      Western blot - Anti-NeuN antibody [EPR21902] (ab236869)
      All lanes : Anti-NeuN antibody [EPR21902] (ab236869) at 1/1000 dilution

      Lane 1 : Human fetal brain lysate
      Lane 2 : Human brain lysate
      Lane 3 : Human cerebellum lysate
      Lane 4 : Human hippocampus lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 34 kDa
      Observed band size: 46,48 kDa why is the actual band size different from the predicted?



      Exposure times: Lanes 1 and 3-4: 15 seconds; Lane 2: 92 seconds.

      Blocking/Dilution buffer: 5% NFDM/TBST.

      The molecular weight observed is consistent with what has been described in the literature (PMID:1483388; PMID:26085943).

    • Immunoprecipitation - Anti-NeuN antibody [EPR21902] (ab236869)
      Immunoprecipitation - Anti-NeuN antibody [EPR21902] (ab236869)

      NeuN was immunoprecipitated from 0.35 mg human fetal brain lysate with ab236869 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab236869 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

      Lane 1: Human fetal brain lysate 10 µg (Input).
      Lane 2: ab236869 IP in human fetal brain lysate.
      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab236869 in human fetal brain lysate.

      Blocking/Dilution buffer: 5% NFDM/TBST.

      Exposure time: 8 seconds.

      The molecular weight observed is consistent with what has been described in the literature (PMID:1483388).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR21902] (ab236869)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR21902] (ab236869)

      Immunohistochemical analysis of paraffin-embedded human cerebellum tissue labeling NeuN with ab236869 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear and cytoplasmic staining in granule cells of human cerebellum (PMID:8813082; PMID:1483388). Counterstained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR21902] (ab236869)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR21902] (ab236869)

      Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling NeuN with ab236869 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear and cytoplasmic staining in neurons of human cerebrum (PMID:8813082; PMID:1483388; PMID:26085943). Counterstained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

       

    • Anti-NeuN antibody [EPR21902] (ab236869)
      Anti-NeuN antibody [EPR21902] (ab236869)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab236869? Please let us know so that we can cite the reference in this datasheet.

    ab236869 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab236869.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com