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  1. Link

    products/primary-antibodies/neun-antibody-neuronal-marker-ab128886.pdf

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Neuroscience Cell Type Marker Neuron marker Soma marker
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Anti-NeuN antibody - Neuronal Marker (ab128886)

  • Datasheet
  • SDS
Reviews (11) Submit a question References (29)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody - Neuronal Marker (ab128886)
  • Western blot - Anti-NeuN antibody - Neuronal Marker (ab128886)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody - Neuronal Marker (ab128886)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody - Neuronal Marker (ab128886)

Key features and details

  • Rabbit polyclonal to NeuN - Neuronal Marker
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-NeuN antibody [EPR21906] - Neuronal Marker (ab236870)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-NeuN antibody - Neuronal Marker
    See all NeuN primary antibodies
  • Description

    Rabbit polyclonal to NeuN - Neuronal Marker
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Sheep, Goat, Horse, Cow, Cat, Dog, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster, Common marmoset
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Soma marker
    • Tags & Cell Markers
    • Cell Type Markers
    • Neuroscience Markers
    • Neuronal
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Forkhead Box
    • Other FOXes
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab128886 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P (8)
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB (1)
Use at an assay dependent concentration. Detects a band of approximately 50 kDa (predicted molecular weight: 34 kDa).
Notes
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration. Detects a band of approximately 50 kDa (predicted molecular weight: 34 kDa).

Target

  • Function

    RNA-binding protein that regulates alternative splicing events.
  • Sequence similarities

    Contains 1 RRM (RNA recognition motif) domain.
  • Cellular localization

    Nucleus. Cytoplasm.
  • Target information above from: UniProt accession A6NFN3 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 101084840 Cat
    • Entrez Gene: 511773 Cow
    • Entrez Gene: 608803 Dog
    • Entrez Gene: 102186905 Goat
    • Entrez Gene: 146713 Human
    • Entrez Gene: 52897 Mouse
    • Entrez Gene: 287847 Rat
    • Entrez Gene: 101111982 Sheep
    • SwissProt: Q0VD23 Cow
    • SwissProt: A6NFN3 Human
    • SwissProt: Q8BIF2 Mouse
    • Unigene: 135229 Human
    • Unigene: 341103 Mouse
    see all
  • Alternative names

    • FLJ56884 antibody
    • FLJ58356 antibody
    • Fox-1 homolog C antibody
    • fox1 homolog C antibody
    • Fox3 antibody
    • FOX3NeuN antibody
    • hexaribonucleotide binding protein 3 antibody
    • HRNBP3 antibody
    • NEUN antibody
    • neuronal nuclei antibody
    • Rbfox3 antibody
    • RFOX3_HUMAN antibody
    • RNA binding protein fox-1 homolog 3 antibody
    • RNA binding protein, fox 1 homolog (C. elegans) 3 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody - Neuronal Marker (ab128886)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody - Neuronal Marker (ab128886)This image is courtesy of Carl Hobbs, King's College London, United Kingdom
    IHC-P image of FOX3/NeuN staining on mouse cerebellum sections using ab128886 (1:2000). The sections were subjected to heat mediated antigen retrieval using citric acid pH6. The sections were blocked using 1% BSA for 10 mins using 21°C. The primary antibody was used at 1:2000 for 2 hours at 21°C. The secondary antibody used was goat polyclonal to rabbit IgG conjugated to biotin.

    See Abreview

  • Western blot - Anti-NeuN antibody - Neuronal Marker (ab128886)
    Western blot - Anti-NeuN antibody - Neuronal Marker (ab128886)
    All lanes : Anti-NeuN antibody - Neuronal Marker (ab128886) at 5 µg/ml

    Lane 1 : Human brain lysate
    Lane 2 : Mouse brain lysate
    Lane 3 : Rat brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti Rabbit IR680 at 1/10000 dilution

    Predicted band size: 34 kDa
    Observed band size: 50 kDa why is the actual band size different from the predicted?
    Additional bands at: 15 kDa, 48 kDa (possible isoform). We are unsure as to the identity of these extra bands.



    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab128886 overnight at 4°C. Antibody binding was detected using Goat anti Rabbit IR680 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody - Neuronal Marker (ab128886)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody - Neuronal Marker (ab128886)This image is courtesy of Carl Hobbs, King's College London, United Kingdom
    IHC-P image of FOX3/NeuN staining on rat brain sections using ab128886 (1:3000). The sections were subjected to heat mediated antigen retrieval using citric acid pH6. The sections were blocked using 1% BSA for 10 mins using 21°C. The primary antibody was used at 1:3000 for 2 hours at 21°C. The secondary antibody used was goat polyclonal to rabbit IgG conjugated to biotin.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody - Neuronal Marker (ab128886)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody - Neuronal Marker (ab128886)This image is courtesy of Carl Hobbs, King's College London, United Kingdom
    IHC-P image of FOX3/NeuN staining on rat brain sections of Cortex (upper image) and lateral ventricle - SVZ (lower image) using ab128886 (1:3000). The sections were subjected to heat mediated antigen retrieval using citric acid pH6. The sections were blocked using 1% BSA for 10 mins using 21°C. The primary antibody was used at 1:3000 for 2 hours at 21°C. The secondary antibody used was goat polyclonal to rabbit IgG conjugated to biotin.

    See Abreview

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (29)

Publishing research using ab128886? Please let us know so that we can cite the reference in this datasheet.

ab128886 has been referenced in 29 publications.

  • Du TT  et al. Anterior thalamic nuclei deep brain stimulation inhibits mossy fiber sprouting via 3',5'-cyclic adenosine monophosphate/protein kinase A signaling pathway in a chronic epileptic monkey model. Chin Med J (Engl) 134:326-333 (2021). PubMed: 33410631
  • Sun CY  et al. Indoxyl sulfate caused behavioral abnormality and neurodegeneration in mice with unilateral nephrectomy. Aging (Albany NY) 13:6681-6701 (2021). PubMed: 33621199
  • Du T  et al. Deep brain stimulation of the anterior nuclei of the thalamus relieves basal ganglia dysfunction in monkeys with temporal lobe epilepsy. CNS Neurosci Ther 27:341-351 (2021). PubMed: 33085171
  • Deng M  et al. Mash-1 modified neural stem cells transplantation promotes neural stem cells differentiation into neurons to further improve locomotor functional recovery in spinal cord injury rats. Gene 781:145528 (2021). PubMed: 33631250
  • Zhang C  et al. Controlled decompression alleviates early brain injury in rabbit intracranial hypertension model by regulating apoptosis/necroptosis. Acta Cir Bras 36:e360406 (2021). PubMed: 34076083
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-10 of 11 Abreviews or Q&A

Western blot abreview for Anti-NeuN antibody - Neuronal Marker

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (Hela Hep G2)
Specification
Hela Hep G2
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jan 07 2015

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-NeuN antibody - Neuronal Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Antigen retrieval step
None
Sample
Sheep Tissue sections (Brain)
Specification
Brain
Permeabilization
Yes - triton-x
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MISS. Cathy Wyse

Verified customer

Submitted May 28 2014

Immunohistochemistry (Frozen sections) abreview for Anti-FOX3/NeuN antibody - Neuronal Marker

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Cerebellum)
Specification
Cerebellum
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
I-Block as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted May 02 2013

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-FOX3/NeuN antibody - Neuronal Marker

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Cerebellum)
Specification
Cerebellum
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric Acid pH6
Permeabilization
No
Blocking step
I-Block as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted May 02 2013

Immunocytochemistry/ Immunofluorescence abreview for Anti-FOX3/NeuN antibody - Neuronal Marker

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (granule neuron cells)
Specification
granule neuron cells
Fixative
Paraformaldehyde
Permeabilization
Yes - PBS + 0.3% Triton
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Apr 23 2013

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-FOX3/NeuN antibody - Neuronal Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Goat Tissue sections (Cerebellum)
Specification
Cerebellum
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Carl Hobbs

Verified customer

Submitted Feb 25 2013

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-FOX3/NeuN antibody - Neuronal Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Marmoset (common) Tissue sections (Cerebellum)
Specification
Cerebellum
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Carl Hobbs

Verified customer

Submitted Feb 25 2013

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-FOX3/NeuN antibody - Neuronal Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (Cerebellum)
Specification
Cerebellum
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Carl Hobbs

Verified customer

Submitted Feb 25 2013

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-FOX3/NeuN antibody - Neuronal Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Cat Tissue sections (Cerebellum)
Specification
Cerebellum
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Carl Hobbs

Verified customer

Submitted Feb 25 2013

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-FOX3/NeuN antibody - Neuronal Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Cerebellum)
Specification
Cerebellum
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Cerebellum
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Carl Hobbs

Verified customer

Submitted Feb 25 2013

1-10 of 11 Abreviews or Q&A

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