Anti-NeuN antibody - Neuronal Marker (ab128886)
Key features and details
- Rabbit polyclonal to NeuN - Neuronal Marker
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
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Overview
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Product name
Anti-NeuN antibody - Neuronal Marker
See all NeuN primary antibodies -
Description
Rabbit polyclonal to NeuN - Neuronal Marker -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Sheep, Goat, Horse, Cow, Cat, Dog, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster, Common marmoset
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab128886 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | (8) |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB | (1) |
Use at an assay dependent concentration. Detects a band of approximately 50 kDa (predicted molecular weight: 34 kDa).
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| Notes |
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IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
Use at an assay dependent concentration. Detects a band of approximately 50 kDa (predicted molecular weight: 34 kDa). |
Target
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Function
RNA-binding protein that regulates alternative splicing events. -
Sequence similarities
Contains 1 RRM (RNA recognition motif) domain. -
Cellular localization
Nucleus. Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 101084840 Cat
- Entrez Gene: 511773 Cow
- Entrez Gene: 608803 Dog
- Entrez Gene: 102186905 Goat
- Entrez Gene: 146713 Human
- Entrez Gene: 52897 Mouse
- Entrez Gene: 287847 Rat
- Entrez Gene: 101111982 Sheep
see all -
Alternative names
- FLJ56884 antibody
- FLJ58356 antibody
- Fox-1 homolog C antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody - Neuronal Marker (ab128886)This image is courtesy of Carl Hobbs, King's College London, United KingdomIHC-P image of FOX3/NeuN staining on mouse cerebellum sections using ab128886 (1:2000). The sections were subjected to heat mediated antigen retrieval using citric acid pH6. The sections were blocked using 1% BSA for 10 mins using 21°C. The primary antibody was used at 1:2000 for 2 hours at 21°C. The secondary antibody used was goat polyclonal to rabbit IgG conjugated to biotin.
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Western blot - Anti-NeuN antibody - Neuronal Marker (ab128886)All lanes : Anti-NeuN antibody - Neuronal Marker (ab128886) at 5 µg/ml
Lane 1 : Human brain lysate
Lane 2 : Mouse brain lysate
Lane 3 : Rat brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti Rabbit IR680 at 1/10000 dilution
Predicted band size: 34 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Additional bands at: 15 kDa, 48 kDa (possible isoform). We are unsure as to the identity of these extra bands.This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab128886 overnight at 4°C. Antibody binding was detected using Goat anti Rabbit IR680 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody - Neuronal Marker (ab128886)This image is courtesy of Carl Hobbs, King's College London, United KingdomIHC-P image of FOX3/NeuN staining on rat brain sections using ab128886 (1:3000). The sections were subjected to heat mediated antigen retrieval using citric acid pH6. The sections were blocked using 1% BSA for 10 mins using 21°C. The primary antibody was used at 1:3000 for 2 hours at 21°C. The secondary antibody used was goat polyclonal to rabbit IgG conjugated to biotin. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody - Neuronal Marker (ab128886)This image is courtesy of Carl Hobbs, King's College London, United KingdomIHC-P image of FOX3/NeuN staining on rat brain sections of Cortex (upper image) and lateral ventricle - SVZ (lower image) using ab128886 (1:3000). The sections were subjected to heat mediated antigen retrieval using citric acid pH6. The sections were blocked using 1% BSA for 10 mins using 21°C. The primary antibody was used at 1:3000 for 2 hours at 21°C. The secondary antibody used was goat polyclonal to rabbit IgG conjugated to biotin.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (36)
ab128886 has been referenced in 36 publications.
- Sultan N & Scheven BA Exploring the potential application of dental pulp stem cells in neuroregenerative medicine. Neural Regen Res 17:775-776 (2022). PubMed: 34472466
- Jhan KY et al. Synaptic loss and progression in mice infected with Angiostrongylus cantonensis in the early stage. J Neuroinflammation 19:85 (2022). PubMed: 35414007
- Kang KR et al. Establishment of Neurotoxicity Assessment Using Microelectrode Array (MEA) with hiPSC-Derived Neurons and Evaluation of New Psychoactive Substances (NPS). Int J Stem Cells 15:258-269 (2022). PubMed: 35769054
- Qiao Y et al. Effects of Tibetan medicine metacinnabar (β-HgS) combined with imipramine or sertraline on depression-like symptoms in mice. Front Pharmacol 13:971243 (2022). PubMed: 36120298
- Li K et al. The Potential Relationship Between HIF-1α and Amino Acid Metabolism After Hypoxic Ischemia and Dual Effects on Neurons. Front Neurosci 15:676553 (2021). PubMed: 34483819