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  1. Link

    products/primary-antibodies/neun-antibody-neuronal-marker-ab134014.pdf

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Neuroscience Cell Type Marker Neuron marker Soma marker
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Anti-NeuN antibody - Neuronal Marker (ab134014)

  • Datasheet
  • SDS
Reviews (8) Submit a question References (13)

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Western blot - Anti-NeuN antibody - Neuronal Marker (ab134014)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody - Neuronal Marker (ab134014)
  • Immunohistochemistry (Frozen sections) - Anti-NeuN antibody - Neuronal Marker (ab134014)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody - Neuronal Marker (ab134014)

Key features and details

  • Chicken polyclonal to NeuN - Neuronal Marker
  • Suitable for: IHC-P, IHC-Fr, WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgY

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-NeuN antibody [EPR21906] - Neuronal Marker (ab236870)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-NeuN antibody - Neuronal Marker
    See all NeuN primary antibodies
  • Description

    Chicken polyclonal to NeuN - Neuronal Marker
  • Host species

    Chicken
  • Tested applications

    Suitable for: IHC-P, IHC-Fr, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to NeuN (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). Corresponding to a region within Human (NP_001076044) and Mouse (NP_001020102).

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 0.9% Sodium chloride
  • Concentration information loading...
  • Purity

    Affinity purified
  • Purification notes

    ab134014 was affinity-purified from the IgY fraction prepared from eggs laid by immunised hens.
  • Clonality

    Polyclonal
  • Isotype

    IgY
  • Research areas

    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Soma marker
    • Tags & Cell Markers
    • Cell Type Markers
    • Neuroscience Markers
    • Neuronal
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Forkhead Box
    • Other FOXes
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors

Associated products

  • Compatible Secondaries

    • Rabbit Anti-Chicken IgY H&L (FITC) (ab6749)
    • Rabbit Anti-Chicken IgY H&L (Biotin) (ab6752)
    • Rabbit Anti-Chicken IgY H&L (HRP) (ab6753)
    • Goat Anti-Chicken IgY H&L (HRP) (ab6877)
    • Goat Anti-Chicken IgY H&L (Biotin) preadsorbed (ab7117)
  • ELISA pair antibody

    • Alexa Fluor® 555 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab207281)
  • Immunizing Peptide (Blocking)

    • Alexa Fluor® 594 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab207279)
  • Isotype control

    • Chicken IgY - Isotype Control (ab50579)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab134014 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P (3)
1/1000 - 1/2000.
IHC-Fr (1)
1/1000 - 1/2000.
WB
1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 34 kDa).Can be blocked with Alexa Fluor® 594 Rabbit monoclonal [EPR12763] to NeuN - Neuronal Marker (ab207279).
Notes
IHC-P
1/1000 - 1/2000.
IHC-Fr
1/1000 - 1/2000.
WB
1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 34 kDa).Can be blocked with Alexa Fluor® 594 Rabbit monoclonal [EPR12763] to NeuN - Neuronal Marker (ab207279).

Target

  • Function

    RNA-binding protein that regulates alternative splicing events.
  • Sequence similarities

    Contains 1 RRM (RNA recognition motif) domain.
  • Cellular localization

    Nucleus. Cytoplasm.
  • Target information above from: UniProt accession A6NFN3 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 146713 Human
    • Entrez Gene: 52897 Mouse
    • Entrez Gene: 287847 Rat
    • SwissProt: A6NFN3 Human
    • SwissProt: Q8BIF2 Mouse
    • Unigene: 135229 Human
    • Unigene: 341103 Mouse
    • Alternative names

      • FLJ56884 antibody
      • FLJ58356 antibody
      • Fox-1 homolog C antibody
      • fox1 homolog C antibody
      • Fox3 antibody
      • FOX3NeuN antibody
      • hexaribonucleotide binding protein 3 antibody
      • HRNBP3 antibody
      • NEUN antibody
      • neuronal nuclei antibody
      • Rbfox3 antibody
      • RFOX3_HUMAN antibody
      • RNA binding protein fox-1 homolog 3 antibody
      • RNA binding protein, fox 1 homolog (C. elegans) 3 antibody
      see all

    Images

    • Western blot - Anti-NeuN antibody - Neuronal Marker (ab134014)
      Western blot - Anti-NeuN antibody - Neuronal Marker (ab134014)
      All lanes : Anti-NeuN antibody - Neuronal Marker (ab134014) at 1/1000 dilution

      Lane 1 : Mouse Brain
      Lane 2 : Rat Brain

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 34 kDa
      Observed band size: 42 kDa why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody - Neuronal Marker (ab134014)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody - Neuronal Marker (ab134014)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of adult mouse cerebral cortex tissue labelling NeuN (red) with ab134014. Tissue was fixed with 4% paraformaldehyde. Green - rabbit anti-Beta-Tubulin 3.

    • Immunohistochemistry (Frozen sections) - Anti-NeuN antibody - Neuronal Marker (ab134014)
      Immunohistochemistry (Frozen sections) - Anti-NeuN antibody - Neuronal Marker (ab134014)

      Immunohistochemistry (Frozen sections) analysis of e18 mouse cochlear ganglion tissue staining NeuN (green) with ab134014. Tissue was fixed with 4% paraformaldehyde. Red - rabbit anti-Beta-Tubulin 3.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody - Neuronal Marker (ab134014)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody - Neuronal Marker (ab134014)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of adult mouse CA1 hippocampus tissue labelling NeuN (red) with ab134014. Tissue was fixed with 4% paraformaldehyde. Green - rabbit anti-Beta-Tubulin 3.

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (13)

    Publishing research using ab134014? Please let us know so that we can cite the reference in this datasheet.

    ab134014 has been referenced in 13 publications.

    • Okada T  et al. Pain induces stable, active microcircuits in the somatosensory cortex that provide a therapeutic target. Sci Adv 7:N/A (2021). PubMed: 33741588
    • Vinnakota C  et al. An 5 GABAA Receptor Inverse Agonist, 5IA, Attenuates Amyloid Beta-Induced Neuronal Death in Mouse Hippocampal Cultures. Int J Mol Sci 21:N/A (2020). PubMed: 32384683
    • Hawrysh PJ & Buck LT Oxygen-sensitive interneurons exhibit increased activity and GABA release during ROS scavenging in the cerebral cortex of the western painted turtle. J Neurophysiol 122:466-479 (2019). PubMed: 31141433
    • Anesten F  et al. Interleukin-6 in the central amygdala is bioactive and co-localised with glucagon-like peptide-1 receptor. J Neuroendocrinol 31:e12722 (2019). PubMed: 31033078
    • Chen W  et al. Astrocytic Insulin-Like Growth Factor-1 Protects Neurons Against Excitotoxicity. Front Cell Neurosci 13:298 (2019). PubMed: 31338023
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-8 of 8 Abreviews or Q&A

    Immunohistochemistry free floating abreview for Anti-NeuN antibody - Neuronal Marker

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry free floating
    Sample
    Mouse Tissue sections (Brain sections)
    Specification
    Brain sections
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted May 27 2022

    Immunocytochemistry/ Immunofluorescence abreview for Anti-NeuN antibody - Neuronal Marker

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (Brain)
    Permeabilization
    No
    Specification
    Brain
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Nov 29 2018

    Immunocytochemistry/ Immunofluorescence abreview for Anti-NeuN antibody - Neuronal Marker

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (Brain Tissue)
    Permeabilization
    Yes - Triton X-100
    Specification
    Brain Tissue
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jun 06 2018

    IHC - Wholemount abreview for Anti-NeuN antibody - Neuronal Marker

    Below Average
    Abreviews
    Abreviews
    abreview image
    Application
    IHC - Wholemount
    Sample
    Rat Tissue (Inferior Colliculus)
    Specification
    Inferior Colliculus
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Lauren Robinson

    Verified customer

    Submitted Feb 27 2018

    Immunohistochemistry (Frozen sections) abreview for Anti-NeuN antibody - Neuronal Marker

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Brain)
    Permeabilization
    Yes - 0.5% TWEEN
    Specification
    Brain
    Blocking step
    BSA as blocking agent for 30 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Carl Hobbs

    Verified customer

    Submitted Sep 26 2014

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-NeuN antibody - Neuronal Marker

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Brain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Specification
    Brain
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Carl Hobbs

    Verified customer

    Submitted Aug 08 2014

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-NeuN antibody - Neuronal Marker

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Brain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Specification
    Brain
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Carl Hobbs

    Verified customer

    Submitted Jul 28 2014

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-NeuN antibody - Neuronal Marker

    Excellent
    Abreviews
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Mouse, whole brain sections, striatum)
    Antigen retrieval step
    None
    Permeabilization
    Yes - Tween-20
    Specification
    Mouse, whole brain sections, striatum
    Blocking step
    Heat-inactivated normal donkey serum in 0.05% PBS-T as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Oct 07 2013

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