Anti-NeuN antibody - Neuronal Marker (ab134014)
Key features and details
- Chicken polyclonal to NeuN - Neuronal Marker
- Suitable for: IHC-P, IHC-Fr, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgY
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Overview
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Product name
Anti-NeuN antibody - Neuronal Marker
See all NeuN primary antibodies -
Description
Chicken polyclonal to NeuN - Neuronal Marker -
Host species
Chicken -
Tested applications
Suitable for: IHC-P, IHC-Fr, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to NeuN (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). Corresponding to a region within Human (NP_001076044) and Mouse (NP_001020102).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituents: PBS, 0.9% Sodium chloride -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
ab134014 was affinity-purified from the IgY fraction prepared from eggs laid by immunised hens. -
Clonality
Polyclonal -
Isotype
IgY -
Research areas
Associated products
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Compatible Secondaries
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ELISA pair antibody
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Immunizing Peptide (Blocking)
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab134014 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | (3) |
1/1000 - 1/2000.
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| IHC-Fr | (1) |
1/1000 - 1/2000.
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| WB |
1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 34 kDa).Can be blocked with Alexa Fluor® 594 Rabbit monoclonal [EPR12763] to NeuN - Neuronal Marker (ab207279).
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| Notes |
|---|
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IHC-P
1/1000 - 1/2000. |
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IHC-Fr
1/1000 - 1/2000. |
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WB
1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 34 kDa).Can be blocked with Alexa Fluor® 594 Rabbit monoclonal [EPR12763] to NeuN - Neuronal Marker (ab207279). |
Target
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Function
RNA-binding protein that regulates alternative splicing events. -
Sequence similarities
Contains 1 RRM (RNA recognition motif) domain. -
Cellular localization
Nucleus. Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 146713 Human
- Entrez Gene: 52897 Mouse
- Entrez Gene: 287847 Rat
- SwissProt: A6NFN3 Human
- SwissProt: Q8BIF2 Mouse
- Unigene: 135229 Human
- Unigene: 341103 Mouse
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Alternative names
- FLJ56884 antibody
- FLJ58356 antibody
- Fox-1 homolog C antibody
see all
Images
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Western blot - Anti-NeuN antibody - Neuronal Marker (ab134014)All lanes : Anti-NeuN antibody - Neuronal Marker (ab134014) at 1/1000 dilution
Lane 1 : Mouse Brain
Lane 2 : Rat Brain
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody - Neuronal Marker (ab134014)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of adult mouse cerebral cortex tissue labelling NeuN (red) with ab134014. Tissue was fixed with 4% paraformaldehyde. Green - rabbit anti-Beta-Tubulin 3.
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Immunohistochemistry (Frozen sections) - Anti-NeuN antibody - Neuronal Marker (ab134014)Immunohistochemistry (Frozen sections) analysis of e18 mouse cochlear ganglion tissue staining NeuN (green) with ab134014. Tissue was fixed with 4% paraformaldehyde. Red - rabbit anti-Beta-Tubulin 3.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody - Neuronal Marker (ab134014)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of adult mouse CA1 hippocampus tissue labelling NeuN (red) with ab134014. Tissue was fixed with 4% paraformaldehyde. Green - rabbit anti-Beta-Tubulin 3.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (13)
ab134014 has been referenced in 13 publications.
- Okada T et al. Pain induces stable, active microcircuits in the somatosensory cortex that provide a therapeutic target. Sci Adv 7:N/A (2021). PubMed: 33741588
- Vinnakota C et al. An 5 GABAA Receptor Inverse Agonist, 5IA, Attenuates Amyloid Beta-Induced Neuronal Death in Mouse Hippocampal Cultures. Int J Mol Sci 21:N/A (2020). PubMed: 32384683
- Hawrysh PJ & Buck LT Oxygen-sensitive interneurons exhibit increased activity and GABA release during ROS scavenging in the cerebral cortex of the western painted turtle. J Neurophysiol 122:466-479 (2019). PubMed: 31141433
- Anesten F et al. Interleukin-6 in the central amygdala is bioactive and co-localised with glucagon-like peptide-1 receptor. J Neuroendocrinol 31:e12722 (2019). PubMed: 31033078
- Chen W et al. Astrocytic Insulin-Like Growth Factor-1 Protects Neurons Against Excitotoxicity. Front Cell Neurosci 13:298 (2019). PubMed: 31338023