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    products/primary-antibodies/neuropeptide-y-antibody-ab30914.pdf

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Anti-Neuropeptide Y antibody (ab30914)

  • Datasheet
  • SDS
Reviews (17)Q&A (3)References (64)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuropeptide Y antibody (ab30914)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuropeptide Y antibody (ab30914)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuropeptide Y antibody (ab30914)

Key features and details

  • Rabbit polyclonal to Neuropeptide Y
  • Suitable for: IHC-P
  • Reacts with: Human
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-Neuropeptide Y antibody [EPR21877] (ab221145)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-Neuropeptide Y antibody
    See all Neuropeptide Y primary antibodies
  • Description

    Rabbit polyclonal to Neuropeptide Y
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Rabbit, Chicken, Guinea pig, Cow, Pigeon, Pig, Zebrafish, Rhesus monkey, Common marmoset
  • Immunogen

    Synthetic peptide corresponding to Pig Neuropeptide Y aa 1 to the C-terminus conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab32971)

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmitter
    • Neuropeptides
    • Hormones
    • Neuroscience
    • Neurotransmitter
    • Neuropeptides
    • More Neuropeptides
    • Cardiovascular
    • Angiogenesis
    • Angiogenic Factors
    • Neuroscience
    • Neurology process
    • Metabolism
    • Cardiovascular
    • Heart
    • Hypertrophy
    • Other
    • Cardiovascular
    • Heart
    • Contractility
    • Inotropics
    • Cardiovascular
    • Vasculature
    • Vasoconstriction
    • Other
    • Metabolism
    • Types of disease
    • Cancer
    • Neuroscience
    • Processes

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Neuropeptide Y protein (ab112330)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab30914 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P (8)
Use at an assay dependent concentration.
Notes
IHC-P
Use at an assay dependent concentration.

Target

  • Function

    NPY is implicated in the control of feeding and in secretion of gonadotrophin-release hormone.
  • Tissue specificity

    One of the most abundant peptides in the nervous system. Also found in some chromaffin cells of the adrenal medulla.
  • Sequence similarities

    Belongs to the NPY family.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P01303 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 396464 Chicken
    • Entrez Gene: 504216 Cow
    • Entrez Gene: 100724008 Guinea pig
    • Entrez Gene: 4852 Human
    • Entrez Gene: 109648 Mouse
    • Entrez Gene: 397304 Pig
    • Entrez Gene: 24604 Rat
    • Entrez Gene: 574114 Rhesus monkey
    • Entrez Gene: 30281 Zebrafish
    • Omim: 162640 Human
    • SwissProt: P28673 Chicken
    • SwissProt: Q6RUW3 Cow
    • SwissProt: P01303 Human
    • SwissProt: P57774 Mouse
    • SwissProt: P01304 Pig
    • SwissProt: P68006 Rabbit
    • SwissProt: P07808 Rat
    • SwissProt: Q9XSW6 Rhesus monkey
    • SwissProt: Q9I8P3 Zebrafish
    • Unigene: 1832 Human
    • Unigene: 154796 Mouse
    • Unigene: 9714 Rat
    • Unigene: 85387 Zebrafish
    see all
  • Alternative names

    • C-flanking peptide of NPY antibody
    • CPON antibody
    • Neuropeptide tyrosine antibody
    • Neuropeptide Y precursor antibody
    • NPY antibody
    • NPY_HUMAN antibody
    • Pro neuropeptide Y antibody
    • PYY 4 antibody
    • PYY4 antibody
    • Y Neuropeptide antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuropeptide Y antibody (ab30914)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuropeptide Y antibody (ab30914)This image is courtesy of an abreview submitted by Carl Hobbs, King's College London, United Kingdom

    Immunohistochemistical detection of Neuropeptide Y using ab30914 on  formaldehyde-fixed paraffin-embedded rat brain sections. The image shows NPY staining in the cerebellum and frontal cortex of the rat brain. Formaldehyde antigen retrieval step: heat mediated in cittric acid ph6 buffer. Blocking step: 1% BSA for 10 mins at room temperature. ab30914 incubated at 1/1000 for 2 hours in TBS/BSA/azide. Secondary Antibody: anti-rabbit IgG conjugated to biotin (1/200)

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuropeptide Y antibody (ab30914)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuropeptide Y antibody (ab30914)This image is courtesy of an abreview submitted by Carl Hobbs, King's College London, United Kingdom

    Immunohistochemistical detection of Neuropeptide Y antibody (ab30914) on formaldehyde fixed paraffin-embedded human foetal wall sectionsAntigen retrieval step: Heat mediated. Buffer: Citric acid pH6. Permeabilization: None.  Blocking step: 1% BSA for 10 mins at 21°C. Primary antibody incubated at 1/1500 for 2 hours at 21°C in TBS/BSA/azide. Abcam's EXPOSE detection kit was used: ab80436. NB: In the submitted image arrowheads indicate positive features: red for ganglion cells in the Submucosa (Meissner's plexus), green for ganglion cells in the Muscularis externa (Auerbach's plexus). There is also a diffuse fine positivity throughout the smooth muscle cells of the externa.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuropeptide Y antibody (ab30914)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuropeptide Y antibody (ab30914)

    IHC image of Neuropeptide Y antibody staining in a section of formalin-fixed paraffin-embedded human cerebral cortex* tissue performed on a Leica BONDTM system using the standard protocol. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab30914, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

Protocols

  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (64)

Publishing research using ab30914? Please let us know so that we can cite the reference in this datasheet.

ab30914 has been referenced in 64 publications.

  • De Felice E  et al. Distinct Pattern of NPY in Gastro-Entero-Pancreatic System of Goat Kids Fed with a New Standardized Red Orange and Lemon Extract (RLE). Animals (Basel) 11:N/A (2021). PubMed: 33572145
  • Kantzer CG  et al. ACSA-2 and GLAST classify subpopulations of multipotent and glial-restricted cerebellar precursors. J Neurosci Res 99:2228-2249 (2021). PubMed: 34060113
  • Zhao S  et al. Cellular and Molecular Probing of Intact Human Organs. Cell 180:796-812.e19 (2020). PubMed: 32059778
  • Wen X  et al. PRADA: Portable Reusable Accurate Diagnostics with nanostar Antennas for multiplexed biomarker screening. Bioeng Transl Med 5:e10165 (2020). PubMed: 33005736
  • Kasai T  et al. Hypothalamic Contribution to Pituitary Functions Is Recapitulated In Vitro Using 3D-Cultured Human iPS Cells. Cell Rep 30:18-24.e5 (2020). PubMed: 31914385
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-10 of 20 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Neuropeptide Y antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
Permeabilization
No
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Apr 11 2022

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Neuropeptide Y antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
Permeabilization
No
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Apr 11 2022

Western blot abreview for Anti-Neuropeptide Y antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Monkey Cell lysate - whole cell (Kidney)
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Loading amount
30 µg
Specification
Kidney
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jun 22 2016

Western blot abreview for Anti-Neuropeptide Y antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Kidney)
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Loading amount
30 µg
Specification
Kidney
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jun 22 2016

Western blot abreview for Anti-Neuropeptide Y antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain)
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Loading amount
30 µg
Specification
Brain
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jun 22 2016

Immunocytochemistry/ Immunofluorescence abreview for Anti-Neuropeptide Y antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Monkey Cell (Kiney)
Permeabilization
Yes - 0.1% troton x-100
Specification
Kiney
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jun 20 2016

Immunohistochemistry (Frozen sections) abreview for Anti-Neuropeptide Y antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (nasal mucosa)
Permeabilization
No
Specification
nasal mucosa
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 24°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Duc Dung Le

Verified customer

Submitted Jun 09 2016

Immunohistochemistry (Frozen sections) abreview for Anti-Neuropeptide Y antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Permeabilization
Yes - 0.3% Triton x 100: 30 min
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted May 04 2016

Immunocytochemistry/ Immunofluorescence abreview for Anti-Neuropeptide Y antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Rat Cortical Cells in culture)
Permeabilization
No
Specification
Rat Cortical Cells in culture
Fixative
PF 4%, Picrid Acid 0,2%
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Ms. Babben Tinner

Verified customer

Submitted Oct 08 2015

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Neuropeptide Y antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (Duodenum (enteric nervous system))
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization
No
Specification
Duodenum (enteric nervous system)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Stephane Lezmi

Verified customer

Submitted May 25 2015

1-10 of 20 Abreviews or Q&A

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