Recombinant Anti-Neuropeptide Y antibody [EPR21877] (ab221145)
![Multiplex immunohistochemistry - Anti-Neuropeptide Y antibody [EPR21877] (ab221145)](https://www.abcam.com/ps/products/221/ab221145/Images/ab221145-505167-anti-pcsk2-antibody-epr23578-19-multiplex-immunohistochemistry-adrenal-gland-human.jpg "Multiplex immunohistochemistry - Anti-Neuropeptide Y antibody [EPR21877] (ab221145)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21877] to Neuropeptide Y
- Suitable for: mIHC, IHC-P, IHC-Fr
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Neuropeptide Y antibody [EPR21877]
See all Neuropeptide Y primary antibodies -
Description
Rabbit monoclonal [EPR21877] to Neuropeptide Y -
Host species
Rabbit -
Tested applications
Suitable for: mIHC, IHC-P, IHC-Frmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human pheochromocytoma; Mouse cerebrum and adrenal gland; Rat adrenal gland tissues. IHC-Fr: Rat and mouse cerebrum tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR21877 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab221145 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| mIHC |
1/2000.
|
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| IHC-P |
1/2000.
1:2000 dilution recommended for human, mouse and rat species. Perform antigen retrieval with universal HIER antigen retrieval reagent (10X) (ab208572). |
|
| IHC-Fr |
1/100 - 1/500.
|
| Notes |
|---|
|
mIHC
1/2000. |
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IHC-P
1/2000. 1:2000 dilution recommended for human, mouse and rat species. Perform antigen retrieval with universal HIER antigen retrieval reagent (10X) (ab208572). |
|
IHC-Fr
1/100 - 1/500. |
Target
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Function
NPY is implicated in the control of feeding and in secretion of gonadotrophin-release hormone. -
Tissue specificity
One of the most abundant peptides in the nervous system. Also found in some chromaffin cells of the adrenal medulla. -
Sequence similarities
Belongs to the NPY family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 4852 Human
- Entrez Gene: 109648 Mouse
- Entrez Gene: 24604 Rat
- Omim: 162640 Human
- SwissProt: P01303 Human
- SwissProt: P57774 Mouse
- SwissProt: P07808 Rat
- Unigene: 1832 Human
see all -
Alternative names
- C-flanking peptide of NPY antibody
- CPON antibody
- Neuropeptide tyrosine antibody
see all
Images
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Multiplex immunohistochemistry - Anti-Neuropeptide Y antibody [EPR21877] (ab221145)
Fluorescence multiplex immunohistochemical analysis of human adrenal gland (formalin-fixed paraffin-embedded section). Panel A shows merged staining of anti-PCSK2 stained on adrenal medulla (ab274418; gray; Opal™690) at 1:2000 (0.263 μg/ml) [Panel B], anti-CYP11A1 stained on adrenal cortex (ab272494; green; Opal™520) at 1:10000 (0.053 μg/ml) [Panel C], and anti-Neuropeptide Y stained on chromaffin cells (ab221145; red; Opal™570) at 1:2000 (0.279 μg/ml) [Panel D] on human adrenal gland. DAPI was used as a nuclear counter stain. Followed by Opal Polymer HRP Ms + Rb secondary antibody. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope. The section was incubated in three rounds of staining: in the order of ab274418, ab272494, and ab221145 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuropeptide Y antibody [EPR21877] (ab221145)](https://www.abcam.com/ps/products/221/ab221145/Images/ab221145-308535-anti-neuropeptide-y-antibody-epr21877-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuropeptide Y antibody [EPR21877] (ab221145)Immunohistochemical analysis of paraffin-embedded human pheochromocytoma tissue labeling Neuropeptide Y with ab221145 at 1/2000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use. Cytoplasmic staining in human pheochromocytoma (PMID: 15623622) is obserevd. Counterstained with hematoxylin. Universal HIER antigen retrieval reagent (10X) (ab208572) was used for the antigen retreival.
Secondary antibody only control: used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). -
![Immunohistochemistry (Frozen sections) - Anti-Neuropeptide Y antibody [EPR21877] (ab221145)](https://www.abcam.com/ps/products/221/ab221145/Images/ab221145-6-anti-neuropeptide-y-antibody-epr21877-immunohistochemistry-frozen-mouse-cerebrum.jpg)
Immunohistochemistry (Frozen sections) - Anti-Neuropeptide Y antibody [EPR21877] (ab221145)Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebrum tissue labeling Neuropeptide Y with ab221145 at 1/500 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution (Green). Positive staining on mouse cerebrum. is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1000 (2 ug/ml) dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
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![Immunohistochemistry (Frozen sections) - Anti-Neuropeptide Y antibody [EPR21877] (ab221145)](https://www.abcam.com/ps/products/221/ab221145/Images/ab221145-308534-anti-neuropeptide-y-antibody-epr21877-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-Neuropeptide Y antibody [EPR21877] (ab221145)Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized rat cerebrum tisssue labeling Neuropeptide Y with ab221145 at 1/100 dilution, followed by AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution. Positive staining in hypothalamic paraventricular nucleus of rat cerebrum (PMID: 19357287) is observed. Counterstained with DAPI (Nuclear).
Perform heat mediated antigen retrieval with sodium citrate buffer (10 mM citrate pH 6.0 + 0.05% Tween-20).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuropeptide Y antibody [EPR21877] (ab221145)](https://www.abcam.com/ps/products/221/ab221145/Images/ab221145-308536-anti-neuropeptide-y-antibody-epr21877-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuropeptide Y antibody [EPR21877] (ab221145)Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Neuropeptide Y with ab221145 at 1/2000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use. Positive staining on mouse cerebrum (PMID: 26444585) is observed. Counterstained with hematoxylin. Universal HIER antigen retrieval reagent (10X) (ab208572) was used for the antigen retrieval.
Secondary antibody only control: used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuropeptide Y antibody [EPR21877] (ab221145)](https://www.abcam.com/ps/products/221/ab221145/Images/ab221145-308537-anti-neuropeptide-y-antibody-epr21877-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuropeptide Y antibody [EPR21877] (ab221145)Immunohistochemical analysis of paraffin-embedded mouse adrenal gland tissue labeling Neuropeptide Y with ab221145 at 1/1000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use. Positive staining on chromaffin cells of mouse adrenal gland medulla (PMID: 26444585) is observed. Counterstained with hematoxylin. Universal HIER antigen retrieval reagent (10X) (ab208572) was used for the antigen retrieval.
Secondary antibody only control: used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuropeptide Y antibody [EPR21877] (ab221145)](https://www.abcam.com/ps/products/221/ab221145/Images/ab221145-308538-anti-neuropeptide-y-antibody-epr21877-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuropeptide Y antibody [EPR21877] (ab221145)Immunohistochemical analysis of paraffin-embedded rat adrenal gland tissue labeling Neuropeptide Y with ab221145 at 1/2000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use. Cytoplasmic staining in chromaffin cells of rat adrenal gland medulla (PMID: 15623622) is observed. Counterstained with hematoxylin. Universal HIER antigen retrieval reagent (10X) (ab208572) was used for the antigen retrieval.
Secondary antibody only control: used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). -
![Anti-Neuropeptide Y antibody [EPR21877] (ab221145)](https://www.abcam.com/ps/products/221/ab221145/Images/ab221145-7-benefits-of-recombinant-antibodies.png)
Anti-Neuropeptide Y antibody [EPR21877] (ab221145)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab221145 has not yet been referenced specifically in any publications.