Anti-Neuropilin 1 antibody (ab25998)
Key features and details
- Rabbit polyclonal to Neuropilin 1
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human, African green monkey
- Isotype: IgG
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- Research with confidence – consistent and reproducible results with every batch
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Overview
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Product name
Anti-Neuropilin 1 antibody
See all Neuropilin 1 primary antibodies -
Description
Rabbit polyclonal to Neuropilin 1 -
Host species
Rabbit -
Specificity
ab25998 is specific for Neuropilin 1. Immunogen sequence has low homology to Neuropilin 2. -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human, African green monkey -
Immunogen
Synthetic peptide, coupled to carrier protein, corresponding to amino acids within the N-terminal CUB domain of human Neuropilin 1. This sequence is highly conserved in rat and mouse Neuropilin 1.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab25998 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/200.
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| IHC-P |
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB | (1) |
1/1000. Predicted molecular weight: 102 kDa.
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| Notes |
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ICC/IF
1/200. |
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IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
1/1000. Predicted molecular weight: 102 kDa. |
Target
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Function
The membrane-bound isoform 1 is a receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. It mediates the chemorepulsant activity of semaphorins. It binds to semaphorin 3A, The PLGF-2 isoform of PGF, The VEGF-165 isoform of VEGF and VEGF-B. Coexpression with KDR results in increased VEGF-165 binding to KDR as well as increased chemotaxis. It may regulate VEGF-induced angiogenesis.
The soluble isoform 2 binds VEGF-165 and appears to inhibit its binding to cells. It may also induce apoptosis by sequestering VEGF-165. May bind as well various members of the semaphorin family. Its expression has an averse effect on blood vessel number and integrity. -
Tissue specificity
The expression of isoforms 1 and 2 does not seem to overlap. Isoform 1 is expressed by the blood vessels of different tissues. In the developing embryo it is found predominantly in the nervous system. In adult tissues, it is highly expressed in heart and placenta; moderately in lung, liver, skeletal muscle, kidney and pancreas; and low in adult brain. Isoform 2 is found in liver hepatocytes, kidney distal and proximal tubules. -
Sequence similarities
Belongs to the neuropilin family.
Contains 2 CUB domains.
Contains 2 F5/8 type C domains.
Contains 1 MAM domain. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 8829 Human
- Omim: 602069 Human
- SwissProt: O14786 Human
- Unigene: 131704 Human
- Unigene: 653996 Human
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Alternative names
- A5 protein antibody
- BDCA4 antibody
- BLOOD DENDRITIC CELL ANTIGEN 4 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuropilin 1 antibody (ab25998)IHC image of ab25998 staining in human heart formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab25998, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Western blot - Anti-Neuropilin 1 antibody (ab25998)All lanes : Anti-Neuropilin 1 antibody (ab25998) at 1/500 dilution
Lane 1 : Untransfected COS-7 cells
Lane 2 : COS-7 cells transfected with HA-tagged Neuropilin 1
Predicted band size: 102 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-Neuropilin 1 antibody (ab25998)ICC/IF image of ab25998 stained SHSY5Y cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab25998, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (14)
ab25998 has been referenced in 14 publications.
- Dong Y et al. Solasonine Induces Apoptosis and Inhibits Proliferation of Bladder Cancer Cells by Suppressing NRP1 Expression. J Oncol 2022:7261486 (2022). PubMed: 35281516
- Gao P et al. STAT6 Upregulates NRP1 Expression in Endothelial Cells and Promotes Angiogenesis. Front Oncol 12:823377 (2022). PubMed: 35600336
- Jin H et al. An Essential NRP1-Mediated Role for Tagln2 in Gastric Cancer Angiogenesis. Front Oncol 11:653246 (2021). PubMed: 34150622
- Creighton BA et al. Giant ankyrin-B mediates transduction of axon guidance and collateral branch pruning factor sema 3A. Elife 10:N/A (2021). PubMed: 34812142
- Chen C et al. Neuropilin-1 is up-regulated by cancer-associated fibroblast-secreted IL-8 and associated with cell proliferation of gallbladder cancer. J Cell Mol Med 24:12608-12618 (2020). PubMed: 32951327