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    products/primary-antibodies/neuropilin-1-antibody-epr3113-ab81321.pdf

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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Neuropilin 1 antibody [EPR3113] (ab81321)

  • Datasheet
  • SDS
Reviews (31)Q&A (4)References (103)

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Western blot - Anti-Neuropilin 1 antibody [EPR3113] (ab81321)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuropilin 1 antibody [EPR3113] (ab81321)
  • Immunocytochemistry/ Immunofluorescence - Anti-Neuropilin 1 antibody [EPR3113] (ab81321)
  • Western blot - Anti-Neuropilin 1 antibody [EPR3113] (ab81321)
  • Flow Cytometry (Intracellular) - Anti-Neuropilin 1 antibody [EPR3113] (ab81321)
  • Immunoprecipitation - Anti-Neuropilin 1 antibody [EPR3113] (ab81321)
  • Western blot - Anti-Neuropilin 1 antibody [EPR3113] (ab81321)
  • Immunocytochemistry/ Immunofluorescence - Anti-Neuropilin 1 antibody [EPR3113] (ab81321)
  • Flow Cytometry (Intracellular) - Anti-Neuropilin 1 antibody [EPR3113] (ab81321)
  • Western blot - Anti-Neuropilin 1 antibody [EPR3113] (ab81321)
  • Immunocytochemistry/ Immunofluorescence - Anti-Neuropilin 1 antibody [EPR3113] (ab81321)
  • Western blot - Anti-Neuropilin 1 antibody [EPR3113] (ab81321)
  • Anti-Neuropilin 1 antibody [EPR3113] (ab81321)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3113] to Neuropilin 1
  • Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF, IP
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free PE

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Alexa Fluor® 647 Anti-Neuropilin 1 antibody [EPR3113] (ab198323)
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Anti-NeuN antibody [EPR12763] - BSA and Azide free (ab209898)

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Overview

  • Product name

    Anti-Neuropilin 1 antibody [EPR3113]
    See all Neuropilin 1 primary antibodies
  • Description

    Rabbit monoclonal [EPR3113] to Neuropilin 1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Monkey, Common marmoset
  • Immunogen

    Synthetic peptide within Human Neuropilin 1 aa 900 to the C-terminus (intracellular). The exact sequence is proprietary.
    Database link: O14786
    (Peptide available as ab189308)

  • Positive control

    • WB: Wild-type A549, MDA-MB-231, HUVEC and HepG2 whole cell lysate (ab7900), human placenta, kidney and heart, mouse heart and kidney and rat heart and kidney tissue lysates. IHC-P: Human liver tissue; Rat brain tissue; Mouse brain tissue. ICC/IF: MCF7 and HUVEC cells; Omentum and effluent-derived mesothelial cells; COS1 fibroblast-like cell line derived from monkey kidney tissue. Flow Cyt (intra): HepG2 and MCF7 cells. IHC-Fr: Human kidney tissue. IP: Mouse heart tissue lysate
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR3113
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Angiogenesis
    • Growth Factors
    • VEGF
    • VEGF Receptors
    • Neuroscience
    • Neurology process
    • Growth and Development
    • Axonal Guidance Proteins
    • Immunology
    • Adaptive Immunity
    • Regulatory T Cells

Associated products

  • Alternative Versions

    • Anti-Neuropilin 1 antibody [EPR3113] - BSA and Azide free (ab184783)
    • Alexa Fluor® 488 Anti-Neuropilin 1 antibody [EPR3113] (ab197644)
    • Alexa Fluor® 647 Anti-Neuropilin 1 antibody [EPR3113] (ab198323)
    • PE Anti-Neuropilin 1 antibody [EPR3113] (ab209445)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96899)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Immunizing Peptide (Blocking)

    • Neuropilin 1 peptide (ab189308)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human NRP1 knockout A549 cell line (ab269507)
  • KO cell lysates

    • Human NRP1 knockout A549 cell lysate (ab269669)
  • Positive Controls

    • Hep G2 whole cell lysate (ab166833)
    • Mouse kidney normal tissue lysate - total protein (ab29305)
    • Mouse heart normal tissue lysate - total protein (ab30291)
    • Mouse heart tissue lysate - total protein (0 days) (ab7193)
    • Mouse heart tissue lysate - total protein (14 days) (ab7194)
    • Mouse kidney tissue lysate - total protein (0 days) (ab7261)
    • Mouse kidney tissue lysate (14 days) (ab7262)
    • Mouse kidney tissue lysate (7 days) (ab7263)
  • Recombinant Protein

    • Recombinant human Neuropilin 1 protein (Fc Chimera Active) (ab221023)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab81321 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/50 - 1/70.

The epitope that the antibody recognizesis intracellular. Fixation and permeabilization are necessary.ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB (6)
1/1000 - 1/2000. Predicted molecular weight: 103 kDa.Can be blocked with Neuropilin 1 peptide (ab189308).
IHC-P (8)
1/100 - 1/400. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

ICC/IF (5)
1/250.
IP
1/30.
Notes
Flow Cyt (Intra)
1/50 - 1/70.

The epitope that the antibody recognizesis intracellular. Fixation and permeabilization are necessary.ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB
1/1000 - 1/2000. Predicted molecular weight: 103 kDa.Can be blocked with Neuropilin 1 peptide (ab189308).
IHC-P
1/100 - 1/400. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

ICC/IF
1/250.
IP
1/30.

Target

  • Function

    The membrane-bound isoform 1 is a receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. It mediates the chemorepulsant activity of semaphorins. It binds to semaphorin 3A, The PLGF-2 isoform of PGF, The VEGF-165 isoform of VEGF and VEGF-B. Coexpression with KDR results in increased VEGF-165 binding to KDR as well as increased chemotaxis. It may regulate VEGF-induced angiogenesis.
    The soluble isoform 2 binds VEGF-165 and appears to inhibit its binding to cells. It may also induce apoptosis by sequestering VEGF-165. May bind as well various members of the semaphorin family. Its expression has an averse effect on blood vessel number and integrity.
  • Tissue specificity

    The expression of isoforms 1 and 2 does not seem to overlap. Isoform 1 is expressed by the blood vessels of different tissues. In the developing embryo it is found predominantly in the nervous system. In adult tissues, it is highly expressed in heart and placenta; moderately in lung, liver, skeletal muscle, kidney and pancreas; and low in adult brain. Isoform 2 is found in liver hepatocytes, kidney distal and proximal tubules.
  • Sequence similarities

    Belongs to the neuropilin family.
    Contains 2 CUB domains.
    Contains 2 F5/8 type C domains.
    Contains 1 MAM domain.
  • Cellular localization

    Secreted and Cell membrane.
  • Target information above from: UniProt accession O14786 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 8829 Human
    • Entrez Gene: 18186 Mouse
    • Entrez Gene: 246331 Rat
    • Omim: 602069 Human
    • SwissProt: O14786 Human
    • SwissProt: P97333 Mouse
    • SwissProt: Q9QWJ9 Rat
    • Unigene: 131704 Human
    • Unigene: 653996 Human
    • Unigene: 271745 Mouse
    • Unigene: 10815 Rat
    see all
  • Alternative names

    • A5 protein antibody
    • BDCA4 antibody
    • BLOOD DENDRITIC CELL ANTIGEN 4 antibody
    • CD304 antibody
    • Neuropilin-1 antibody
    • Neuropilin1 antibody
    • NP1 antibody
    • NPN1 antibody
    • NRP 1 antibody
    • NRP antibody
    • NRP1 antibody
    • NRP1_HUMAN antibody
    • transmembrane receptor antibody
    • Vascular endothelial cell growth factor 165 receptor antibody
    • VEGF165R antibody
    see all

Images

  • Western blot - Anti-Neuropilin 1 antibody [EPR3113] (ab81321)
    Western blot - Anti-Neuropilin 1 antibody [EPR3113] (ab81321)
    All lanes : Anti-Neuropilin 1 antibody [EPR3113] (ab81321) at 1/1000 dilution

    Lane 1 : Wild-type A549 cell lysate
    Lane 2 : NRP1 knockout A549 cell lysate
    Lane 3 : MDA-MB-231 cell lysate
    Lane 4 : SK-BR-3 cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 103 kDa
    Observed band size: 125-135 kDa why is the actual band size different from the predicted?



    False colour image of Western blot: Anti-Neuropilin 1 antibody [EPR3113] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab81321 was shown to bind specifically to Neuropilin 1. A band was observed at 125/135 kDa in wild-type A549 cell lysates with no signal observed at this size in NRP1 knockout cell line ab269507 (knockout cell lysate ab269669). To generate this image, wild-type and NRP1 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuropilin 1 antibody [EPR3113] (ab81321)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuropilin 1 antibody [EPR3113] (ab81321)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling Neuropilin 1 with purified ab81321 at 1/400. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Immunocytochemistry/ Immunofluorescence - Anti-Neuropilin 1 antibody [EPR3113] (ab81321)
    Immunocytochemistry/ Immunofluorescence - Anti-Neuropilin 1 antibody [EPR3113] (ab81321)Image from Pérez-Lozano ML et al., PLoS One. 2013;8(4):e60776. Fig 5.; doi: 10.1371/journal.pone.0060776.

    The expression of Neuropilin 1, VEGFR-2, and VEGF was analyzed by immunofluorescence microscopy in omentum and effluent-derived mesothelial cells (MCs). MCs were double stained for Neuropilin 1 (green) and VEGFR-2 (red), and single stained for VEGF (green). Nuclei were stained with DAPI. Neuropilin 1 and VEGF show a membrane distribution in omentum and epithelioid MCs (b, c, h, i). During in vitro (e, f) and ex vivo (k, l) MMT both proteins change their localization and are internalized. The expression of VEGFR-2 is down-regulated but it does not show differences in localization during in vitro (a, d) and ex vivo (g, j) MMT.

  • Western blot - Anti-Neuropilin 1 antibody [EPR3113] (ab81321)
    Western blot - Anti-Neuropilin 1 antibody [EPR3113] (ab81321)
    All lanes : Anti-Neuropilin 1 antibody [EPR3113] (ab81321) at 1/10000 dilution (purified)

    Lane 1 : Mouse heart tissue lysate
    Lane 2 : Rat heart tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

    Predicted band size: 103 kDa
    Observed band size: 120 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

  • Flow Cytometry (Intracellular) - Anti-Neuropilin 1 antibody [EPR3113] (ab81321)
    Flow Cytometry (Intracellular) - Anti-Neuropilin 1 antibody [EPR3113] (ab81321)

    Intracellular Flow Cytometry analysis of MCF7 cells labelling Neuropilin 1 with purified ab81321 at 1/70 (red). Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies. 

     

     

  • Immunoprecipitation - Anti-Neuropilin 1 antibody [EPR3113] (ab81321)
    Immunoprecipitation - Anti-Neuropilin 1 antibody [EPR3113] (ab81321)

    Neuropilin 1 was immunoprecipitated from 0.35mg mouse heart lysate with ab81321 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab81321 at 1/1000 dilution (0.77 μg/mL). VeriBlot for IP Detection Reagent (HRP) (ab131366) was used as the secondary antibody at 1/1000 dilution.

    Lane 1: Mouse heart tissue lysate 10 μg
    Lane 2: Mouse heart tissue lysate
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab81321 in mouse heart lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

  • Western blot - Anti-Neuropilin 1 antibody [EPR3113] (ab81321)
    Western blot - Anti-Neuropilin 1 antibody [EPR3113] (ab81321)
    Anti-Neuropilin 1 antibody [EPR3113] (ab81321) at 1/10000 dilution (purified) + Human heart tissue lysate at 20 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

    Predicted band size: 103 kDa
    Observed band size: 120 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry/ Immunofluorescence - Anti-Neuropilin 1 antibody [EPR3113] (ab81321)
    Immunocytochemistry/ Immunofluorescence - Anti-Neuropilin 1 antibody [EPR3113] (ab81321)

    ICC/IF image of unpurified ab81321 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (unpurified ab81321, 5µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG - H&L, pre-adsorbed (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Flow Cytometry (Intracellular) - Anti-Neuropilin 1 antibody [EPR3113] (ab81321)
    Flow Cytometry (Intracellular) - Anti-Neuropilin 1 antibody [EPR3113] (ab81321)

    Overlay histogram showing HepG2 cells stained with unpurified ab81321 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (unpurified ab81321, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (0.5µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a significantly decreased signal in HepG2 cells fixed with methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions. 

     

     

  • Western blot - Anti-Neuropilin 1 antibody [EPR3113] (ab81321)
    Western blot - Anti-Neuropilin 1 antibody [EPR3113] (ab81321)
    Anti-Neuropilin 1 antibody [EPR3113] (ab81321) at 1/2000 dilution (purified) + Human placenta tissue lysate at 20 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

    Predicted band size: 103 kDa
    Observed band size: 120 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry/ Immunofluorescence - Anti-Neuropilin 1 antibody [EPR3113] (ab81321)
    Immunocytochemistry/ Immunofluorescence - Anti-Neuropilin 1 antibody [EPR3113] (ab81321)

    Immunocytochemistry/Immunofluorescence analysis of HUVEC cells labelling Neuropilin 1 with purified ab81321 at 1/250. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500) were also used.

    Control 1: primary antibody (1/250) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

    Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).

  • Western blot - Anti-Neuropilin 1 antibody [EPR3113] (ab81321)
    Western blot - Anti-Neuropilin 1 antibody [EPR3113] (ab81321)
    All lanes : Anti-Neuropilin 1 antibody [EPR3113] (ab81321) at 1/1000 dilution (unpurified)

    Lane 1 : Human placenta lysate
    Lane 2 : HUVEC cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : Mouse heart tissue lysate
    Lane 5 : Mouse kidney tissue lysate
    Lane 6 : Rat heart tissue lysate
    Lane 7 : Rat kidney tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 103 kDa
    Observed band size: 120 kDa why is the actual band size different from the predicted?

  • Anti-Neuropilin 1 antibody [EPR3113] (ab81321)
    Anti-Neuropilin 1 antibody [EPR3113] (ab81321)

Protocols

  • Flow cytometry protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (103)

Publishing research using ab81321? Please let us know so that we can cite the reference in this datasheet.

ab81321 has been referenced in 103 publications.

  • Bernatz S  et al. Influence of VEGF-A, VEGFR-1-3, and neuropilin 1-2 on progression-free: and overall survival in WHO grade II and III meningioma patients. J Mol Histol 52:233-243 (2021). PubMed: 33528717
  • Li X  et al. Circular RNA circVMA21 ameliorates lipopolysaccharide (LPS)-induced acute kidney injury by targeting the miR-199a-5p/NRP1 axis in sepsis. Biochem Biophys Res Commun 548:174-181 (2021). PubMed: 33647793
  • Yang X  et al. NRP1 and MMP9 are dual targets of RNA-binding protein QKI5 to alter VEGF-R/ NRP1 signalling in trophoblasts in preeclampsia. J Cell Mol Med 25:5655-5670 (2021). PubMed: 33942999
  • Shao L  et al. Effects of MLL5 and HOXA regulated by NRP1 on radioresistance in A549. Oncol Lett 21:403 (2021). PubMed: 33777226
  • Andriessen EMMA  et al. Myeloid-resident neuropilin-1 promotes choroidal neovascularization while mitigating inflammation. EMBO Mol Med 13:e11754 (2021). PubMed: 33876574
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
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1-10 of 35 Abreviews or Q&A

Immunohistochemistry (Frozen sections) abreview for Anti-Neuropilin 1 antibody [EPR3113]

Average
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (E14.5 Mouse Embryo (Sagittal section))
Permeabilization
Yes - Triton
Specification
E14.5 Mouse Embryo (Sagittal section)
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 0.25% · Temperature: 21°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dr. Alain Chedotal

Verified customer

Submitted Feb 25 2021

IHC - Wholemount abreview for Anti-Neuropilin 1 antibody [EPR3113]

Average
Abreviews
Abreviews
abreview image
Application
IHC - Wholemount
Sample
Mouse Embryo (E12.5 Mouse Embryo)
Specification
E12.5 Mouse Embryo
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dr. Alain Chedotal

Verified customer

Submitted Feb 25 2021

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Neuropilin 1 antibody [EPR3113]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Rat bearing rat brain tumor 9L)
Antigen retrieval step
None
Permeabilization
Yes - triton 0.2%
Specification
Rat bearing rat brain tumor 9L
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MRS. Francoise Geffroy

Verified customer

Submitted Jun 03 2020

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Neuropilin 1 antibody [EPR3113]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Human Tissue sections (Mouse bearing human tumor U87)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate 10 mM
Permeabilization
Yes - triton 1%
Specification
Mouse bearing human tumor U87
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5 · Temperature: 20°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MRS. Francoise Geffroy

Verified customer

Submitted Apr 30 2020

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Neuropilin 1 antibody [EPR3113]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Mouse bearing Mouse brain tumor GL261)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate 10mM
Permeabilization
Yes - triton 1%
Specification
Mouse bearing Mouse brain tumor GL261
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5 · Temperature: 20°C
Fixative
Paraformaldehyde
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The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MRS. Francoise Geffroy

Verified customer

Submitted Apr 30 2020

Immunocytochemistry/ Immunofluorescence abreview for Anti-Neuropilin 1 antibody [EPR3113]

Average
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Brain cell culture)
Permeabilization
Yes - 0.05% Tween 20
Specification
Brain cell culture
Blocking step
Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 21 2020

Immunocytochemistry/ Immunofluorescence abreview for Anti-Neuropilin 1 antibody [EPR3113]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Brain cell culture)
Permeabilization
Yes - 0.05% tween 20
Specification
Brain cell culture
Blocking step
Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 18 2020

Immunocytochemistry/ Immunofluorescence abreview for Anti-Neuropilin 1 antibody [EPR3113]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Kidney H9 Organoids)
Permeabilization
Yes - 0.05% Tween 20
Specification
Kidney H9 Organoids
Blocking step
Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 13 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Neuropilin 1 antibody [EPR3113]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA Buffer, pH 9.0
Permeabilization
Yes - 0.05% tween 20
Specification
Kidney
Blocking step
Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 11 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Neuropilin 1 antibody [EPR3113]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA Buffer, pH 9.0
Permeabilization
Yes - 0.05% tween 20
Specification
Brain
Blocking step
Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 11 2020

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