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RecombinantRabMAb

Recombinant Anti-Neurturin antibody [EPR23301-10] (ab274417)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurturin antibody [EPR23301-10] (ab274417)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurturin antibody [EPR23301-10] (ab274417)
  • Western blot - Anti-Neurturin antibody [EPR23301-10] (ab274417)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurturin antibody [EPR23301-10] (ab274417)
  • Western blot - Anti-Neurturin antibody [EPR23301-10] (ab274417)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurturin antibody [EPR23301-10] (ab274417)
  • Anti-Neurturin antibody [EPR23301-10] (ab274417)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23301-10] to Neurturin
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-Neurturin antibody [EPR23301-10]
    See all Neurturin primary antibodies

  • Description

    Rabbit monoclonal [EPR23301-10] to Neurturin

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human heart, testis and brain tissue lysates; Mouse embryo and heart tissue lysates; Rat heart and brain tissue lysates; SH-SY5Y and Neuro-2a whole cell lysates. IHC-P: Human cerebrum and testis tissue; Mouse testis tissue; Rat testis tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR23301-10

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab274417 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000. Detects a band of approximately 16, 28 kDa (predicted molecular weight: 22 kDa).
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
WB
1/1000. Detects a band of approximately 16, 28 kDa (predicted molecular weight: 22 kDa).
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Application notes
Is unsuitable for Flow Cyt,ICC/IF or IP.

Target

  • Function

    Supports the survival of sympathetic neurons in culture. May regulate the development and maintenance of the CNS. Might control the size of non-neuronal cell population such as haemopoietic cells.

  • Involvement in disease

    Defects in NRTN are a cause of Hirschsprung disease (HSCR) [MIM:142623]. In association with mutations of RET gene, and possibly with other loci, defects in NRTN are involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction.

  • Sequence similarities

    Belongs to the TGF-beta family. GDNF subfamily.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    • Alternative names

      • Neurturin antibody
      • Neurturin precursor antibody
      • NRTN antibody
      • NRTN_HUMAN antibody
      • NTN antibody
      • prepro-neurturin antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurturin antibody [EPR23301-10] (ab274417)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurturin antibody [EPR23301-10] (ab274417)

      Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling Neurturin with ab274417 at 1/500 (1.094 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse testis. The section was incubated with ab274417 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurturin antibody [EPR23301-10] (ab274417)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurturin antibody [EPR23301-10] (ab274417)

      Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Neurturin with ab274417 at 1/500 (1.094 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human cerebrum (PMID: 25841760). The section was incubated with ab274417 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Western blot - Anti-Neurturin antibody [EPR23301-10] (ab274417)
      Western blot - Anti-Neurturin antibody [EPR23301-10] (ab274417)
      All lanes : Anti-Neurturin antibody [EPR23301-10] (ab274417) at 1/1000 dilution

      Lane 1 : Mouse embryo tissue lysate
      Lane 2 : Mouse heart tissue lysate
      Lane 3 : Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate
      Lane 4 : Rat heart tissue lysate
      Lane 5 : Rat brain tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

      Predicted band size: 22 kDa
      Observed band size: 16,28 kDa why is the actual band size different from the predicted?



      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      Lanes 1-3 were developed using a higher sensitivity ECL substrate.

      16-kDa Monomeric and 28-kDa dimeric form of Neurturin are observed. The molecular weight observed is consistent with what has been described in the literature (PMID: 15829225, PMID: 24067900).

      Exposure times: Lane 1-3: 3 minutes Lane 4-5: 37 seconds.

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurturin antibody [EPR23301-10] (ab274417)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurturin antibody [EPR23301-10] (ab274417)

      Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling Neurturin with ab274417 at 1/500 (1.094 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human testis. The section was incubated with ab274417 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Western blot - Anti-Neurturin antibody [EPR23301-10] (ab274417)
      Western blot - Anti-Neurturin antibody [EPR23301-10] (ab274417)
      All lanes : Anti-Neurturin antibody [EPR23301-10] (ab274417) at 1/1000 dilution

      Lane 1 : Human heart tissue lysate
      Lane 2 : Human testis tissue lysate
      Lane 3 : Human brain tissue lysate
      Lane 4 : SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : VeriBlot for IP secondary antibody(HRP)(ab131366) at 1/1000 dilution

      Predicted band size: 22 kDa
      Observed band size: 16,28 kDa why is the actual band size different from the predicted?



      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      This blot was developed using a higher sensitivity ECL substrate.

      16-kDa Monomeric and 28-kDa dimeric form of Neurturin are observed. The molecular weight observed is consistent with what has been described in the literature (PMID: 15829225, PMID: 24067900).

      Exposure time: 3 minutes.

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurturin antibody [EPR23301-10] (ab274417)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurturin antibody [EPR23301-10] (ab274417)

      Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling Neurturin with ab274417 at 1/500 (1.094 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat testis (PMID: 10415380). The section was incubated with ab274417 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Anti-Neurturin antibody [EPR23301-10] (ab274417)
      Anti-Neurturin antibody [EPR23301-10] (ab274417)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab274417? Please let us know so that we can cite the reference in this datasheet.

    ab274417 has not yet been referenced specifically in any publications.

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