Anti-Neutrophil Bactericidal Protein BP30 antibody (ab196732)
Key features and details
- Rabbit polyclonal to Neutrophil Bactericidal Protein BP30
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Neutrophil Bactericidal Protein BP30 antibody -
Description
Rabbit polyclonal to Neutrophil Bactericidal Protein BP30 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment corresponding to Human Neutrophil Bactericidal Protein BP30 (C terminal).
Database link: P17213 -
Positive control
- Jurkat, HepG2, SK-Hep1, mouse liver and mouse thymus extracts; Mouse lung tissue; A549 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
PBS without Mg2+ and Ca2+. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab196732 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/2000. Predicted molecular weight: 54 kDa.
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| IHC-P |
1/50 - 1/200.
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| ICC/IF |
1/50 - 1/200.
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| Notes |
|---|
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WB
1/500 - 1/2000. Predicted molecular weight: 54 kDa. |
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IHC-P
1/50 - 1/200. |
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ICC/IF
1/50 - 1/200. |
Target
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Function
The cytotoxic action of BPI is limited to many species of Gram-negative bacteria; this specificity may be explained by a strong affinity of the very basic N-terminal half for the negatively charged lipopolysaccharides that are unique to the Gram-negative bacterial outer envelope. Has antibacterial activity against the Gram-nagative bacterium P.aeruginosa, this activity is inhibited by LPS from P.aeruginosa. -
Tissue specificity
Restricted to cells of the myeloid series. -
Sequence similarities
Belongs to the BPI/LBP/Plunc superfamily. BPI/LBP family. -
Domain
The N-terminal region may be exposed to the interior of the granule, whereas the C-terminal portion may be embedded in the membrane. During phagocytosis and degranulation, proteases may be released and activated and cleave BPI at the junction of the N-and C-terminal portions of the molecule, providing controlled release of the N-terminal antibacterial fragment when bacteria are ingested. -
Cellular localization
Secreted. Cytoplasmic granule membrane. Membrane-associated in polymorphonuclear Leukocytes (PMN) granules. - Information by UniProt
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Database links
- Entrez Gene: 671 Human
- Entrez Gene: 329547 Mouse
- Omim: 109195 Human
- SwissProt: P17213 Human
- SwissProt: Q67E05 Mouse
- Unigene: 529019 Human
- Unigene: 260883 Mouse
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Alternative names
- Bactericidal permeability-increasing protein antibody
- Bactericidal/permeability increasing protein antibody
- BPI antibody
see all
Images
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Western blot - Anti-Neutrophil Bactericidal Protein BP30 antibody (ab196732)All lanes : Anti-Neutrophil Bactericidal Protein BP30 antibody (ab196732) at 1/500 dilution
Lane 1 : Jurkat cell extract
Lane 2 : HepG2 cell extract
Lane 3 : SK-Hep1 cell extract
Lane 4 : Mouse liver extract
Lane 5 : Mouse thymus extract
Predicted band size: 54 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neutrophil Bactericidal Protein BP30 antibody (ab196732)Immunohistochemical analysis of paraffin-embedded Mouse liver cancer tissue labeling Neutrophil Bactericidal Protein BP30 with ab196732 at 1/200 dilution.
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Immunocytochemistry/ Immunofluorescence - Anti-Neutrophil Bactericidal Protein BP30 antibody (ab196732)Immunofluorescent analysis of A549 cells labeling Neutrophil Bactericidal Protein BP30 with ab196732 at 1/50 dilution.
Blue: DAPI for nuclear staining.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab196732 has not yet been referenced specifically in any publications.