Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
RecombinantRabMAb

Recombinant Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738)

Submit a review Submit a question References (12)
Western blot - Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738)
  • Flow Cytometry (Intracellular) - Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738)
  • Western blot - Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738)
  • Western blot - Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738)
  • Immunocytochemistry/ Immunofluorescence - Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738)
  • Immunoprecipitation - Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738)
  • Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14122] to NFIB / NF1B2
  • Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF, IP
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 APC Carrier Free HRP PE

Overview

  • Product name

    Anti-NFIB / NF1B2 antibody [EPR14122]
    See all NFIB / NF1B2 primary antibodies

  • Description

    Rabbit monoclonal [EPR14122] to NFIB / NF1B2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa, rat C6, rat PC12 and mouse NIH 3T3 cell lysates; Human fetal brain lysate; Human breast and Mouse liver tissue; HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR14122

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab186738 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
Use at an assay dependent concentration.
WB
1/1000 - 1/10000. Detects a band of approximately 47,53,55 kDa (predicted molecular weight: 47 kDa).
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100.
IP
1/30 - 1/50.
Notes
Flow Cyt (Intra)
Use at an assay dependent concentration.
WB
1/1000 - 1/10000. Detects a band of approximately 47,53,55 kDa (predicted molecular weight: 47 kDa).
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100.
IP
1/30 - 1/50.

Target

  • Function

    Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.

  • Sequence similarities

    Belongs to the CTF/NF-I family.
    Contains 1 CTF/NF-I DNA-binding domain.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • CCAAT Box Binding Transcription Factor antibody
      • CCAAT-box-binding transcription factor antibody
      • CTF antibody
      • HMGIC/NFIB antibody
      • NF-I/B antibody
      • NF1-B antibody
      • NF1B antibody
      • NF1B2 antibody
      • NFI-B antibody
      • NFI-RED antibody
      • Nfib antibody
      • NFIB_HUMAN antibody
      • NFIB2 antibody
      • NFIB3 antibody
      • Nuclear factor 1 B-type antibody
      • Nuclear factor 1/B antibody
      • Nuclear Factor 1B antibody
      • Nuclear Factor I B antibody
      • Nuclear factor I/B antibody
      • TGGCA Binding Protein antibody
      • TGGCA-binding protein antibody
      • TRANSCRIPTION FACTOR NFIB antibody
      see all

    Images

    • Western blot - Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738)
      Western blot - Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738)
      Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738) at 1/5000 dilution + Human fetal brain at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/1000 dilution

      Developed using the ECL technique.

      Predicted band size: 47 kDa
      Observed band size: 47 kDa

    • Flow Cytometry (Intracellular) - Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738)
      Flow Cytometry (Intracellular) - Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738)

      ab186738 staining NFIB / NF1B2 in the human cell line HeLa (human cervix adenocarcinoma) by intracellular flow cytometry. Cells were fixed with 4% paraformaldehyde, permiabilised with 90% methanol and the sample was incubated with the primary antibody at a dilution of 1/70. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody. 

      Isoytype control: Rabbit monoclonal IgG (Black) 

      Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue) 

       

       

    • Western blot - Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738)
      Western blot - Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738)
      Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738) at 1/5000 dilution + HeLa cell lysate at 10 µg

      Secondary
      goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

      Developed using the ECL technique.

      Predicted band size: 47 kDa
      Observed band size: 47, 53, 55 kDa why is the actual band size different from the predicted?

    • Western blot - Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738)
      Western blot - Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738)
      All lanes : Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738) at 1/1000 dilution

      Lane 1 : C6 cell lysate
      Lane 2 : PC-12 cell lysate
      Lane 3 : NIH/3T3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

      Developed using the ECL technique.

      Predicted band size: 47 kDa
      Observed band size: 47 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738)

      Immunohistochemical analysis of paraffin-embedded, Human breast tissue labeling NFIB / NF1B2 with ab186738 at a 1/500 dilution. Counter stained with hematoxylin.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738)

      Immunohistochemical analysis of paraffin-embedded, mouse liver tissue labeling NFIB / NF1B2 with ab186738 at a 1/500 dilution. Counter stained with hematoxylin.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738)
      Immunocytochemistry/ Immunofluorescence - Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738)

      Immunofluorescence analysis of, paraformaldehyde-fixed, HeLa cells labeling NFIB / NF1B2 with ab186738 at a 1/100 dilution. As secondary antibody goat anti-rabbit IgG (Alexa Fluor®555) was used at a 1/200. In blue DAPI staining.

    • Immunoprecipitation - Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738)
      Immunoprecipitation - Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738)

      Western blot analysis on immunoprecipitation from 1) Human fetal brain lysate and 2) PBS, labeling NFIB / NF1B2 using ab186738 at 1/50 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG at a 1/1500 dilution.

    • Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738)
      Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738)

    References (12)

    Publishing research using ab186738? Please let us know so that we can cite the reference in this datasheet.

    ab186738 has been referenced in 12 publications.

    • Cheng R  et al. Nuclear factor I/B mediates epithelial-mesenchymal transition in human melanoma cells through ZEB1. Oncol Lett 21:81 (2021). PubMed: 33363618
    • Caramello A  et al. Dentate gyrus development requires a cortical hem-derived astrocytic scaffold. Elife 10:N/A (2021). PubMed: 33393905
    • Yang H  et al. NFIB promotes the progression of gastric cancer by upregulating circMAP7D1 to stabilize HER2 mRNA. Mol Med Rep 23:N/A (2021). PubMed: 33576439
    • Wang N  et al. NFIB promotes the migration and progression of kidney renal clear cell carcinoma by regulating PINK1 transcription. PeerJ 9:e10848 (2021). PubMed: 33981484
    • Pozo K  et al. ASCL1, NKX2-1, and PROX1 co-regulate subtype-specific genes in small-cell lung cancer. iScience 24:102953 (2021). PubMed: 34466783
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab186738.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com