Anti-NFkB Inducing Kinase NIK antibody (ab19144)
Key features and details
- Rabbit polyclonal to NFkB Inducing Kinase NIK
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
-
Product name
Anti-NFkB Inducing Kinase NIK antibody
See all NFkB Inducing Kinase NIK primary antibodies -
Description
Rabbit polyclonal to NFkB Inducing Kinase NIK -
Host species
Rabbit -
Specificity
This antibody recognises human NFkB Inducing Kinase NIK. -
Tested applications
Suitable for: IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide:
SFAWSWRVKHGQLENRP
, corresponding to amino acids 931-947 of Human NFkB Inducing Kinase NIK -
Positive control
- Whole cell lysate of 293.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab19144 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
| ICC/IF |
Use a concentration of 1 - 5 µg/ml.
|
|
| WB |
1/500. Detects a band of approximately 105 kDa (predicted molecular weight: 105.4 kDa).
|
| Notes |
|---|
|
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
ICC/IF
Use a concentration of 1 - 5 µg/ml. |
|
WB
1/500. Detects a band of approximately 105 kDa (predicted molecular weight: 105.4 kDa). |
Target
-
Function
Lymphotoxin beta-activated kinase which seems to be exclusively involved in the activation of NF-kappa-B and its transcriptional activity. Promotes proteolytic processing of NFKB2/P100, which leads to activation of NF-kappa-B via the non-canonical pathway. Could act in a receptor-selective manner. -
Tissue specificity
Weakly expressed in testis, small intestine, spleen, thymus, peripheral blood leukocytes, prostate, ovary and colon. -
Sequence similarities
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsAutophosphorylated. Phosphorylation at Thr-559 is required to activates its kinase activity and 'Lys-63'-linked polyubiquitination. Phosphorylated by CHUK/IKKA leading to MAP3K14 destabilization.
Ubiquitinated. Undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. 'Lys-48'-linked polyubiquitination leads to its degradation by the proteasome, while 'Lys-63'-linked polyubiquitination stabilizes and activates it. -
Cellular localization
Cytoplasm. - Information by UniProt
-
Database links
- Entrez Gene: 9020 Human
- Omim: 604655 Human
- SwissProt: Q99558 Human
- Unigene: 404183 Human
- Unigene: 668927 Human
- Unigene: 735695 Human
-
Alternative names
- MAP3K14 antibody
- FTDCR1B antibody
- HS antibody
see all
Images
-
Western blot - Anti-NFkB Inducing Kinase NIK antibody (ab19144)Anti-NFkB Inducing Kinase NIK antibody (ab19144) at 1/500 dilution + NIK-transfected 293 cell lysate
Predicted band size: 105.4 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-NFkB Inducing Kinase NIK antibody (ab19144)ICC/IF image of ab19144 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab19144, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFkB Inducing Kinase NIK antibody (ab19144)IHC image of ab19144 staining in human cervix formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab19144, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
Datasheets and documents
-
Datasheet download
References (4)
ab19144 has been referenced in 4 publications.
- Li J et al. Hypoxic colorectal cancer-derived extracellular vesicles deliver microRNA-361-3p to facilitate cell proliferation by targeting TRAF3 via the noncanonical NF-?B pathways. Clin Transl Med 11:e349 (2021). PubMed: 33784010
- Das R et al. Lymphotoxin-ß receptor-NIK signaling induces alternative RELB/NF-?B2 activation to promote metastatic gene expression and cell migration in head and neck cancer. Mol Carcinog 58:411-425 (2019). PubMed: 30488488
- Maracle CX et al. Noncanonical NF-?B signaling in microvessels of atherosclerotic lesions is associated with inflammation, atheromatous plaque morphology and myocardial infarction. Atherosclerosis 270:33-41 (2018). IHC-P ; Human . PubMed: 29407886
- Maracle CX et al. Silencing NIK potentiates anti-VEGF therapy in a novel 3D model of colorectal cancer angiogenesis. Oncotarget 9:28445-28455 (2018). PubMed: 29983872
