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    products/primary-antibodies/nfkb-p105--p50-antibody-e381-ab32360.pdf

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Cell Biology Apoptosis Intracellular NFkB p50
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-NFkB p105 / p50 antibody [E381] (ab32360)

  • Datasheet
  • SDS
Reviews (21)Q&A (1)References (161)

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Western blot - Anti-NFkB p105 / p50 antibody [E381] (ab32360)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFkB p105 / p50 antibody [E381] (ab32360)
  • Western blot - Anti-NFkB p105 / p50 antibody [E381] (ab32360)
  • Western blot - Anti-NFkB p105 / p50 antibody [E381] (ab32360)
  • Western blot - Anti-NFkB p105 / p50 antibody [E381] (ab32360)
  • Western blot - Anti-NFkB p105 / p50 antibody [E381] (ab32360)
  • Western blot - Anti-NFkB p105 / p50 antibody [E381] (ab32360)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFkB p105 / p50 antibody [E381] (ab32360)
  • Anti-NFkB p105 / p50 antibody [E381] (ab32360)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [E381] to NFkB p105 / p50
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-NFkB p105 / p50 antibody [E381]
    See all NFkB p105 / p50 primary antibodies
  • Description

    Rabbit monoclonal [E381] to NFkB p105 / p50
  • Host species

    Rabbit
  • Specificity

    This antibody will detect both forms: p50 and p105.
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, MCF-7 and PC-12 cell lysates. IHC-P: human prostate carcinoma and bladder carcinoma tissues.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    E381
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Intracellular
    • NFkB
    • p50
    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • NFkB Pathway
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • NFkB pathway
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors
    • Cancer
    • Signal transduction
    • Nuclear signaling
    • NFkB pathway
    • Cancer
    • Cell Death
    • Apoptosis
    • Apoptosis Markers
    • NFkB
    • p50

Associated products

  • Alternative Versions

    • HRP Anti-NFkB p105 / p50 antibody [E381] (ab195854)
    • Anti-NFkB p105 / p50 antibody [E381] - BSA and Azide free (ab220803)
  • Assay kits

    • Human NFkB p50 Transcription Factor Assay Kit (ab133105)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
    • Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96899)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab32360 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (11)
1/1000 - 1/10000. Detects a band of approximately 50, 105 kDa (predicted molecular weight: 50 kDa).
IHC-P (5)
1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

Notes
WB
1/1000 - 1/10000. Detects a band of approximately 50, 105 kDa (predicted molecular weight: 50 kDa).
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

Application notes
Is unsuitable for Flow Cyt.

Target

  • Function

    NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and RelB-p50 complexes are transcriptional activators. The NF-kappa-B p50-p50 homodimer is a transcriptional repressor, but can act as a transcriptional activator when associated with BCL3. NFKB1 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p105 and generation of p50 by a cotranslational processing. The proteasome-mediated process ensures the production of both p50 and p105 and preserves their independent function, although processing of NFKB1/p105 also appears to occur post-translationally. p50 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. In a complex with MAP3K8, NFKB1/p105 represses MAP3K8-induced MAPK signaling; active MAP3K8 is released by proteasome-dependent degradation of NFKB1/p105.
  • Sequence similarities

    Contains 7 ANK repeats.
    Contains 1 death domain.
    Contains 1 RHD (Rel-like) domain.
  • Domain

    The C-terminus of p105 might be involved in cytoplasmic retention, inhibition of DNA-binding, and transcription activation.
    Glycine-rich region (GRR) appears to be a critical element in the generation of p50.
  • Post-translational
    modifications

    While translation occurs, the particular unfolded structure after the GRR repeat promotes the generation of p50 making it an acceptable substrate for the proteasome. This process is known as cotranslational processing. The processed form is active and the unprocessed form acts as an inhibitor (I kappa B-like), being able to form cytosolic complexes with NF-kappa B, trapping it in the cytoplasm. Complete folding of the region downstream of the GRR repeat precludes processing.
    Phosphorylation at 'Ser-903' and 'Ser-907' primes p105 for proteolytic processing in response to TNF-alpha stimulation. Phosphorylation at 'Ser-927' and 'Ser-932' are required for BTRC/BTRCP-mediated proteolysis.
    Polyubiquitination seems to allow p105 processing.
    S-nitrosylation of Cys-61 affects DNA binding.
  • Cellular localization

    Nucleus. Cytoplasm. Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor.
  • Target information above from: UniProt accession P19838 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 4790 Human
    • Entrez Gene: 18033 Mouse
    • Entrez Gene: 81736 Rat
    • Omim: 164011 Human
    • SwissProt: P19838 Human
    • SwissProt: P25799 Mouse
    • SwissProt: Q63369 Rat
    • Unigene: 618430 Human
    • Unigene: 256765 Mouse
    • Unigene: 2411 Rat
    see all
  • Alternative names

    • DKFZp686C01211 antibody
    • DNA binding factor KBF1 antibody
    • DNA binding factor KBF1 EBP1 antibody
    • DNA-binding factor KBF1 antibody
    • EBP 1 antibody
    • EBP-1 antibody
    • EBP1 antibody
    • KBF1 antibody
    • MGC54151 antibody
    • nf b antibody
    • NF kappa B antibody
    • NF kappaB antibody
    • NF kappabeta antibody
    • NF kB1 antibody
    • NF-kappaB antibody
    • NFkappaB antibody
    • NFKB 1 antibody
    • NFKB p105 antibody
    • NFKB p50 antibody
    • NFKB-p105 antibody
    • Nfkb1 antibody
    • NFKB1_HUMAN antibody
    • Nuclear factor kappa B DNA binding subunit antibody
    • Nuclear factor kappa-B antibody
    • Nuclear factor kappa-B, subunit 1 antibody
    • Nuclear factor NF kappa B p105 subunit antibody
    • Nuclear factor NF kappa B p50 subunit antibody
    • Nuclear factor NF-kappa-B p50 subunit antibody
    • Nuclear factor of kappa light chain gene enhancer in B cells 1 antibody
    • Nuclear factor of kappa light polypeptide gene enhancer in B cells 1 antibody
    • Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 antibody
    • p105 antibody
    • p50 antibody
    • p84/NF-kappa-B1 p98 antibody
    • Transcription factor NFKB1 antibody
    see all

Images

  • Western blot - Anti-NFkB p105 / p50 antibody [E381] (ab32360)
    Western blot - Anti-NFkB p105 / p50 antibody [E381] (ab32360)
    All lanes : Anti-NFkB p105 / p50 antibody [E381] (ab32360)

    Lane 1 : Wild-type HAP1 cell lysate
    Lane 2 : NFkB p105 / p50 knockout HAP1 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 50 kDa



    Lanes 1 - 4: Merged signal (red and green). Green - ab32360 observed at 120, 50 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab32360 was shown to specifically react with NFkB p105 / p50 when NFkB p105 / p50 knockout samples were used. Wild-type and NFkB p105 / p50 knockout samples were subjected to SDS-PAGE. ab32360 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10 000 respectively and and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFkB p105 / p50 antibody [E381] (ab32360)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFkB p105 / p50 antibody [E381] (ab32360)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bladder carcinoma tissue labelling NFkB p105 / p50 with purified ab32360 at 1/250. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Western blot - Anti-NFkB p105 / p50 antibody [E381] (ab32360)
    Western blot - Anti-NFkB p105 / p50 antibody [E381] (ab32360)
    All lanes : Anti-NFkB p105 / p50 antibody [E381] (ab32360) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : NFKB1 CRISPR/Cas9 edited HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 50 kDa
    Observed band size: 105 kDa why is the actual band size different from the predicted?



    Lanes 1- 2: Merged signal (red and green). Green - ab32360 observed at 105 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

     ab32360 was shown to react with NFkB p105 / p50 in wild-type HeLa cells in western blot. The band observed in CRISPR/Cas9 edited cell line ab264823 (CRISPR/Cas9 edited cell lysate ab257003) lane below 105kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type HeLa and NFKB1 CRISPR/Cas9 HeLa HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab32360 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-NFkB p105 / p50 antibody [E381] (ab32360)
    Western blot - Anti-NFkB p105 / p50 antibody [E381] (ab32360)
    Anti-NFkB p105 / p50 antibody [E381] (ab32360) at 1/10000 dilution (purified) + HeLa cell lysate at 20 µg

    Secondary
    Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 50 kDa
    Observed band size: 105,50 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-NFkB p105 / p50 antibody [E381] (ab32360)
    Western blot - Anti-NFkB p105 / p50 antibody [E381] (ab32360)
    Anti-NFkB p105 / p50 antibody [E381] (ab32360) at 1/50000 dilution (purified) + MCF-7 cell lysate at 10 µg

    Secondary
    Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 50 kDa
    Observed band size: 105 kDa why is the actual band size different from the predicted?
    Additional bands at: 50 kDa (possible non-specific binding)



    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-NFkB p105 / p50 antibody [E381] (ab32360)
    Western blot - Anti-NFkB p105 / p50 antibody [E381] (ab32360)
    All lanes : Anti-NFkB p105 / p50 antibody [E381] (ab32360) at 1/10000 dilution (purified)

    Lane 1 : PC-12 cell lysate
    Lane 2 : NIH/3T3 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 50 kDa
    Observed band size: 105 kDa why is the actual band size different from the predicted?
    Additional bands at: 50 kDa (possible non-specific binding)



    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-NFkB p105 / p50 antibody [E381] (ab32360)
    Western blot - Anti-NFkB p105 / p50 antibody [E381] (ab32360)
    Anti-NFkB p105 / p50 antibody [E381] (ab32360) at 1/5000 dilution (unpurified) + HeLa cell lysate

    Predicted band size: 50 kDa
    Observed band size: 105,50 kDa why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFkB p105 / p50 antibody [E381] (ab32360)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFkB p105 / p50 antibody [E381] (ab32360)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling NFkB p105 / p50 with unpurified ab32360 at a dilution of 1/250.

  • Anti-NFkB p105 / p50 antibody [E381] (ab32360)
    Anti-NFkB p105 / p50 antibody [E381] (ab32360)

Protocols

  • IHC-P protocol

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (161)

Publishing research using ab32360? Please let us know so that we can cite the reference in this datasheet.

ab32360 has been referenced in 161 publications.

  • Cao W  et al. Naringin Targets NFKB1 to Alleviate Oxygen-Glucose Deprivation/Reoxygenation-Induced Injury in PC12 Cells Via Modulating HIF-1a/AKT/mTOR-Signaling Pathway. J Mol Neurosci 71:101-111 (2021). PubMed: 32557145
  • Zou XS  et al. Pomelo peel oil alleviates cerebral NLRP3 inflammasome activation in a cardiopulmonary resuscitation rat model. Exp Ther Med 21:233 (2021). PubMed: 33603841
  • Huang D  et al. Casticin protected against neuronal injury and inhibited the TLR4/NF-?B pathway after middle cerebral artery occlusion in rats. Pharmacol Res Perspect 9:e00752 (2021). PubMed: 33704926
  • Zhang L  et al. The anti-breast cancer property of physcion via oxidative stress-mediated mitochondrial apoptosis and immune response. Pharm Biol 59:303-310 (2021). PubMed: 33715588
  • Zhou Z  et al. LncRNA XIST promotes inflammation by downregulating GRa expression in the adenoids of children with OSAHS. Exp Ther Med 21:500 (2021). PubMed: 33791009
View all Publications for this product

Customer reviews and Q&As

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1-10 of 22 Abreviews or Q&A

Immunocytochemistry abreview for Anti-NFkB p105 / p50 antibody [E381]

Below Average
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry
Sample
Dog Cell (melanoma cells)
Permeabilization
Yes - Triton X-100
Specification
melanoma cells
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Apr 08 2022

Western blot abreview for Anti-NFkB p105 / p50 antibody [E381]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Dog Cell lysate - whole cell (melanoma ces)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
10 µg
Treatment
100 pM canine recombinant IL-1β
Specification
melanoma ces
Blocking step
blockace as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 100%
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. 令 中野

Verified customer

Submitted Apr 08 2022

Western blot abreview for Anti-NFkB p105 / p50 antibody [E381]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (293T)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
50000 cells
Specification
293T
Blocking step
Milk as blocking agent for 45 minute(s) · Concentration: 1.5% · Temperature: 21°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Aug 14 2019

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-NFkB p105 / p50 antibody [E381]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Bone marrow)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: sodiumcitrate buffer pH 6.0
Permeabilization
No
Specification
Bone marrow
Blocking step
Optiview Peroxidase Inhibitor as blocking agent for 4 minute(s) · Concentration: 3.0% · Temperature: 36°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Oct 16 2018

Western blot abreview for Anti-NFkB p105 / p50 antibody [E381]

Average
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (mouse neural stem cell)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
40 µg
Specification
mouse neural stem cell
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jun 26 2018

Immunocytochemistry/ Immunofluorescence abreview for Anti-NFkB p105 / p50 antibody [E381]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (A375 melanoma cell line)
Permeabilization
Yes - 0,5% Triton-100x
Specification
A375 melanoma cell line
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Cristina Penas Lago

Verified customer

Submitted Oct 05 2017

ChIP abreview for Anti-NFkB p105 / p50 antibody [E381]

Good
Abreviews
Abreviews
abreview image
Application
ChIP
Sample
Rabbit Cell lysate - nuclear (Primary mouse CD4+ Th1 Cells, non activated and ac)
Negative control
Insulin gene (inactive in T cells) (Primer Ins neg).
Specification
Primary mouse CD4+ Th1 Cells, non activated and ac
Detection step
Real-time PCR
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% Formaldehyde
Positive control
Known p50 binding site at the transcriptional start site of NFkB2 (Primer NFkB2_TSS_1) and in the Bcl3 intron (Primer Bcl3 Intr_2).
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Kathrin Davari

Verified customer

Submitted Sep 28 2016

ChIP abreview for Anti-NFkB p105 / p50 antibody [E381]

Good
Abreviews
Abreviews
abreview image
Application
ChIP
Sample
Mouse Cell lysate - nuclear (Primary CD4+ Th1 cells, non activated or activated)
Negative control
Insulin gene (not active in T cells) (Primer Ins_neg).
Specification
Primary CD4+ Th1 cells, non activated or activated
Detection step
Real-time PCR
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% Formaldehyde
Positive control
Known p50 target site at the transcriptional start site of NFkB2 (Primer: NFkB2_TSS_1).
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Kathrin Davari

Verified customer

Submitted Sep 27 2016

Western blot abreview for Anti-NFkB p105 / p50 antibody [E381]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rat Tissue lysate - whole (Skeletal muscle)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
20 µg
Specification
Skeletal muscle
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
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The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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Verified customer

Submitted Feb 16 2016

Immunocytochemistry/ Immunofluorescence abreview for Anti-NFkB p105 / p50 antibody [E381]

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Mueller cell line MIO-M1)
Permeabilization
Yes - 0.1% Tx-100
Specification
Mueller cell line MIO-M1
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
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The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Dec 31 2015

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