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    products/primary-antibodies/nfkb-p105--p50-antibody-nf5e6-ab230846.pdf

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Cell Biology Apoptosis Intracellular NFkB p50
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Anti-NFkB p105 / p50 antibody [NF5E6] (ab230846)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFkB p105 / p50 antibody [NF5E6] (ab230846)

    Key features and details

    • Rat monoclonal [NF5E6] to NFkB p105 / p50
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG1

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    Overview

    • Product name

      Anti-NFkB p105 / p50 antibody [NF5E6]
      See all NFkB p105 / p50 primary antibodies
    • Description

      Rat monoclonal [NF5E6] to NFkB p105 / p50
    • Host species

      Rat
    • Tested applications

      Suitable for: IHC-Pmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Recombinant fragment corresponding to Human NFkB p105/ p50 aa 1-100.
      Database link: P19838

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • Positive control

      • IHC-P: Human placenta tissue.
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.4
      Preservative: 0.05% Sodium azide
      Constituent: Tris glycine
    • Concentration information loading...
    • Purity

      Protein G purified
    • Clonality

      Monoclonal
    • Clone number

      NF5E6
    • Isotype

      IgG1
    • Research areas

      • Cell Biology
      • Apoptosis
      • Intracellular
      • NFkB
      • p50
      • Immunology
      • Innate Immunity
      • Macrophage / Inflamm.
      • Signal Transduction
      • Signaling Pathway
      • Nuclear Signaling
      • NFkB Pathway
      • Epigenetics and Nuclear Signaling
      • Nuclear Signaling Pathways
      • NFkB pathway
      • Epigenetics and Nuclear Signaling
      • Transcription
      • Transcription Factors
      • Cancer
      • Signal transduction
      • Nuclear signaling
      • NFkB pathway
      • Kits/ Lysates/ Other
      • Kits
      • Cell Signaling Kits
      • Other Cell Signaling Kits
      • Cancer
      • Cell Death
      • Apoptosis
      • Apoptosis Markers
      • NFkB
      • p50

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157)
      • Goat Anti-Rat IgG H&L (HRP) (ab205720)
    • Recombinant Protein

      • Recombinant Human NFkB p105 / p50 protein (ab114185)
    • Related Products

      • Recombinant Human NFkB p105 / p50 protein (ab114185)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab230846 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-P
    Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
    Notes
    IHC-P
    Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    Target

    • Function

      NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and RelB-p50 complexes are transcriptional activators. The NF-kappa-B p50-p50 homodimer is a transcriptional repressor, but can act as a transcriptional activator when associated with BCL3. NFKB1 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p105 and generation of p50 by a cotranslational processing. The proteasome-mediated process ensures the production of both p50 and p105 and preserves their independent function, although processing of NFKB1/p105 also appears to occur post-translationally. p50 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. In a complex with MAP3K8, NFKB1/p105 represses MAP3K8-induced MAPK signaling; active MAP3K8 is released by proteasome-dependent degradation of NFKB1/p105.
    • Sequence similarities

      Contains 7 ANK repeats.
      Contains 1 death domain.
      Contains 1 RHD (Rel-like) domain.
    • Domain

      The C-terminus of p105 might be involved in cytoplasmic retention, inhibition of DNA-binding, and transcription activation.
      Glycine-rich region (GRR) appears to be a critical element in the generation of p50.
    • Post-translational
      modifications

      While translation occurs, the particular unfolded structure after the GRR repeat promotes the generation of p50 making it an acceptable substrate for the proteasome. This process is known as cotranslational processing. The processed form is active and the unprocessed form acts as an inhibitor (I kappa B-like), being able to form cytosolic complexes with NF-kappa B, trapping it in the cytoplasm. Complete folding of the region downstream of the GRR repeat precludes processing.
      Phosphorylation at 'Ser-903' and 'Ser-907' primes p105 for proteolytic processing in response to TNF-alpha stimulation. Phosphorylation at 'Ser-927' and 'Ser-932' are required for BTRC/BTRCP-mediated proteolysis.
      Polyubiquitination seems to allow p105 processing.
      S-nitrosylation of Cys-61 affects DNA binding.
    • Cellular localization

      Nucleus. Cytoplasm. Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor.
    • Target information above from: UniProt accession P19838 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 4790 Human
      • Omim: 164011 Human
      • SwissProt: P19838 Human
      • Unigene: 618430 Human
      • Alternative names

        • DKFZp686C01211 antibody
        • DNA binding factor KBF1 antibody
        • DNA binding factor KBF1 EBP1 antibody
        • DNA-binding factor KBF1 antibody
        • EBP 1 antibody
        • EBP-1 antibody
        • EBP1 antibody
        • KBF1 antibody
        • MGC54151 antibody
        • nf b antibody
        • NF kappa B antibody
        • NF kappaB antibody
        • NF kappabeta antibody
        • NF kB1 antibody
        • NF-kappaB antibody
        • NFkappaB antibody
        • NFKB 1 antibody
        • NFKB p105 antibody
        • NFKB p50 antibody
        • NFKB-p105 antibody
        • Nfkb1 antibody
        • NFKB1_HUMAN antibody
        • Nuclear factor kappa B DNA binding subunit antibody
        • Nuclear factor kappa-B antibody
        • Nuclear factor kappa-B, subunit 1 antibody
        • Nuclear factor NF kappa B p105 subunit antibody
        • Nuclear factor NF kappa B p50 subunit antibody
        • Nuclear factor NF-kappa-B p50 subunit antibody
        • Nuclear factor of kappa light chain gene enhancer in B cells 1 antibody
        • Nuclear factor of kappa light polypeptide gene enhancer in B cells 1 antibody
        • Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 antibody
        • p105 antibody
        • p50 antibody
        • p84/NF-kappa-B1 p98 antibody
        • Transcription factor NFKB1 antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFkB p105 / p50 antibody [NF5E6] (ab230846)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFkB p105 / p50 antibody [NF5E6] (ab230846)

        Formalin-fixed, paraffin-embedded human placenta tissue stained for NFkB p50 using ab230846 at 10 μg/ml in immunohistochemical analysis.

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet download

        Download

      References (0)

      Publishing research using ab230846? Please let us know so that we can cite the reference in this datasheet.

      ab230846 has not yet been referenced specifically in any publications.

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