Recombinant Anti-NG2 antibody [EPR20244] (ab183929)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NG2 antibody [EPR20244] (ab183929)](https://www.abcam.com/ps/products/183/ab183929/Images/ab183929-288274-anti-ng2-antibody-epr20244-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NG2 antibody [EPR20244] (ab183929)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20244] to NG2
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-NG2 antibody [EPR20244]
See all NG2 primary antibodies -
Description
Rabbit monoclonal [EPR20244] to NG2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: A-375 and MeWo whole cell lysates; Human skin lysate. IHC-P: Human melanoma and glioma tissues. ICC/IF: A-375 and MeWo cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20244 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab183929 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Predicted molecular weight: 251 kDa.
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| ICC/IF |
1/100.
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| IHC-P |
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| Notes |
|---|
|
WB
1/1000. Predicted molecular weight: 251 kDa. |
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ICC/IF
1/100. |
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IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N-terminus growth factors, extracellular matrix proteases modulating their activity. May regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. May activate FAK and ERK1/ERK2 signaling cascades. -
Tissue specificity
Detected only in malignant melanoma cells. -
Sequence similarities
Contains 15 CSPG (NG2) repeats.
Contains 2 laminin G-like domains. -
Post-translational
modificationsO-glycosylated; contains glycosaminoglycan chondroitin sulfate which are required for proper localization and function in stress fiber formation (By similarity). Involved in interaction with MMP16 and ITGA4.
Phosphorylation by PRKCA regulates its subcellular location and function in cell motility. -
Cellular localization
Apical cell membrane. Cell projection > lamellipodium membrane. Localized at the apical plasma membrane it relocalizes to the lamellipodia of astrocytoma upon phosphorylation by PRKCA. Localizes to the retraction fibers. Localizes to the plasma membrane of oligodendrocytes. - Information by UniProt
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Database links
- Entrez Gene: 1464 Human
- Omim: 601172 Human
- SwissProt: Q6UVK1 Human
- Unigene: 513044 Human
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Alternative names
- 4732461B14Rik antibody
- AN2 antibody
- AN2 proteoglycan antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NG2 antibody [EPR20244] (ab183929)
Immunohistochemical analysis of paraffin-embedded human melanoma tissue labeling NG2 with ab183929 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous and weak cytoplasmic staining on tumor cells of human melanoma (PMID: 24258997). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Western blot - Anti-NG2 antibody [EPR20244] (ab183929)](https://www.abcam.com/ps/products/183/ab183929/Images/ab183929-288275-anti-ng2-antibody-epr20244-western-blot.jpg)
Western blot - Anti-NG2 antibody [EPR20244] (ab183929)All lanes : Anti-NG2 antibody [EPR20244] (ab183929) at 1/1000 dilution
Lane 1 : A-375 (human malignant melanoma cell line), whole cell lysate
Lane 2 : Human skin lysate
Lane 3 : MeWo (human malignant melanoma cell line), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Lanes 1 & 3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Lane 2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 251 kDa
Observed band size: 270 kDa why is the actual band size different from the predicted?Exposure times: Lanes 1,3: 1 minute; Lane 2: 3 minutes.
Blocking/Dilution buffer: 5% NFDM/TBST.
This image of lane 3 was performed on a BIO-RAD® ChemiDoc™ MP instrument.
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![Immunocytochemistry/ Immunofluorescence - Anti-NG2 antibody [EPR20244] (ab183929)](https://www.abcam.com/ps/products/183/ab183929/Images/ab183929-288272-anti-ng2-antibody-epr20244-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-NG2 antibody [EPR20244] (ab183929)Immunofluorescent analysis of 100% methanol-fixed A-375 (human malignant melanoma cell line) cells labeling NG2 with ab183929 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membrane staining on A-375 cell line.
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NG2 antibody [EPR20244] (ab183929)](https://www.abcam.com/ps/products/183/ab183929/Images/ab183929-288273-anti-ng2-antibody-epr20244-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NG2 antibody [EPR20244] (ab183929)Immunohistochemical analysis of paraffin-embedded human glioma tissue labeling NG2 with ab183929 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous staining on blood vessels of human glioma (PMID: 24386429). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunocytochemistry/ Immunofluorescence - Anti-NG2 antibody [EPR20244] (ab183929)](https://www.abcam.com/ps/products/183/ab183929/Images/ab183929-288271-anti-ng2-antibody-epr20244-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-NG2 antibody [EPR20244] (ab183929)Immunofluorescent analysis of 100% methanol-fixed MeWo (human malignant melanoma cell line) cells labeling NG2 with ab183929 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membrane staining on MeWo cell line.
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
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![Anti-NG2 antibody [EPR20244] (ab183929)](https://www.abcam.com/ps/products/183/ab183929/Images/ab183929-6-benefits-of-recombinant-antibodies.png)
Anti-NG2 antibody [EPR20244] (ab183929)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab183929 has been referenced in 1 publication.
- Wang Q et al. Changes in Tumor-Infiltrating Lymphocytes and Vascular Normalization in Breast Cancer Patients After Neoadjuvant Chemotherapy and Their Correlations With DFS. Front Oncol 9:1545 (2019). PubMed: 32039020