Name: Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4]
Brand: RabMAb
SKU: ab308306
Rating: 2 (1 reviews)

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Western blot - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] (ab308306)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR27397-4] to Nicotinic Acetylcholine Receptor alpha 1/CHRNA1
Suitable for: WB, IHC-Fr, IHC-P
Reacts with: Mouse, Rat, Human

Carrier Free

Product name

Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4]
See all Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 primary antibodies
Description

Rabbit monoclonal [EPR27397-4] to Nicotinic Acetylcholine Receptor alpha 1/CHRNA1
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-Fr, IHC-Pmore details
Unsuitable for: Flow Cyt (Intra),ICC/IF or IP
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human, mouse and rat skeletal muscle tissue lysate. IHC-P: Human, mouse and rat skeletal muscle tissue. HEK-293T transfected with a CHRNA1 expression vector containing a his tag. IHC-Fr: Mouse and rat skeletal muscle (fresh) tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR27397-4
Isotype

IgG
Research areas

Alternative Versions
- Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] - BSA and Azide free (ab308307)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081)
- Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Related Products
- Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab308306 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/1000. Predicted molecular weight: 54 kDa.
IHC-Fr		1/100.
IHC-P	(1)	1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Notes
WB 1/1000. Predicted molecular weight: 54 kDa.
IHC-Fr 1/100.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Application notes

Is unsuitable for Flow Cyt (Intra),ICC/IF or IP.

Function

After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
Tissue specificity

Isoform 1 is only expressed in skeletal muscle. Isoform 2 is constitutively expressed in skeletal muscle, brain, heart, kidney, liver, lung and thymus.
Involvement in disease

Defects in CHRNA1 are a cause of multiple pterygium syndrome lethal type (MUPSL) [MIM:253290]. Multiple pterygia are found infrequently in children with arthrogryposis and in fetuses with fetal akinesia syndrome. In lethal multiple pterygium syndrome there is intrauterine growth retardation, multiple pterygia, and flexion contractures causing severe arthrogryposis and fetal akinesia. Subcutaneous edema can be severe, causing fetal hydrops with cystic hygroma and lung hypoplasia. Oligohydramnios and facial anomalies are frequent.
Note=The alpha subunit is the main focus for antibody binding in myasthenia gravis. Myasthenia gravis is characterized by sporadic muscular fatigability and weakness, occurring chiefly in muscles innervated by cranial nerves, and characteristically improved by cholinesterase-inhibiting drugs.
Defects in CHRNA1 are a cause of congenital myasthenic syndrome slow-channel type (SCCMS) [MIM:601462]. SCCMS is the most common congenital myasthenic syndrome. Congenital myasthenic syndromes are characterized by muscle weakness affecting the axial and limb muscles (with hypotonia in early-onset forms), the ocular muscles (leading to ptosis and ophthalmoplegia), and the facial and bulbar musculature (affecting sucking and swallowing, and leading to dysphonia). The symptoms fluctuate and worsen with physical effort. SCCMS is caused by kinetic abnormalities of the AChR, resulting in prolonged endplate currents and prolonged AChR channel opening episodes.
Defects in CHRNA1 are a cause of congenital myasthenic syndrome fast-channel type (FCCMS) [MIM:608930]. FCCMS is a congenital myasthenic syndrome characterized by kinetic abnormalities of the AChR. In most cases, FCCMS is due to mutations that decrease activity of the AChR by slowing the rate of opening of the receptor channel, speeding the rate of closure of the channel, or decreasing the number of openings of the channel during ACh occupancy. The result is failure to achieve threshold depolarization of the endplate and consequent failure to fire an action potential.
Sequence similarities

Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-1/CHRNA1 sub-subfamily.
Cellular localization

Cell junction > synapse > postsynaptic cell membrane. Cell membrane.
Target information above from: UniProt accession P02708 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 1134 Human
- Entrez Gene: 11435 Mouse
- Entrez Gene: 79557 Rat
- Omim: 100690 Human
- SwissProt: P02708 Human
- SwissProt: P04756 Mouse
- SwissProt: P25108 Rat
- Unigene: 434479 Human
- Unigene: 4583 Mouse
- Unigene: 44633 Rat
see all
Alternative names
- Acetylcholine receptor subunit alpha antibody
- ACHA_HUMAN antibody
- AChR antibody
- ACHRA antibody
- ACHRD antibody
- CHNRA antibody
- Cholinergic receptor nicotinic alpha 1 subunit antibody
- Cholinergic receptor nicotinic alpha polypeptide 1 antibody
- Cholinergic receptor, nicotinic, alpha polypeptide 1 (muscle) antibody
- Chrna1 antibody
- CMS1A antibody
- CMS1B antibody
- CMS2A antibody
- FCCMS antibody
- Nicotinic cholinergic receptor alpha 1 antibody
- SCCMS antibody
- Schizophrenia neurophysiologic defect candidate antibody
see all

Western blot - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] (ab308306)

All lanes : Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] (ab308306) at 1/1000 dilution

Lane 1 : Human skeletal muscle tissue lysate
Lane 2 : Human testis tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 54 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Negative control: testis (PMID: 34228850).

This antibody can only recognize the functional skeletal muscle-specific isoform (isoform 1) dimers of CHRNA1. Functional CHRNA1 can form dimers with delta subunits (CHRND) or epsilon subunits (CHRNE) (PMID:29874875; PMID:36634413; PMID:1694127; PMID:11836518; PMID:7695910).

Samples are non-boiled as boiling may cause protein aggregation.

In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution.

Exposure time: 70 seconds.
Western blot - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] (ab308306)

All lanes : Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] (ab308306) at 1/1000 dilution

Lane 1 : Mouse skeletal muscle tissue lysate
Lane 2 : Mouse testis tissue lysate
Lane 3 : Mouse kidney tissue lysate
Lane 4 : Rat skeletal muscle tissue lysate
Lane 5 : Rat testis tissue lysate
Lane 6 : Rat kidney tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 54 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Negative control: testis and kidney (PMID: 34228850).

This antibody can only recognize the functional skeletal muscle-specific isoform (isoform 1) dimers of CHRNA1. Functional CHRNA1 can form dimers with delta subunits (CHRND) or epsilon subunits (CHRNE) (PMID:29874875; PMID:36634413; PMID:1694127; PMID:11836518; PMID:7695910).

Samples are non-boiled as boiling may cause protein aggregation.

In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution.

Exposure time: 37 seconds.
Immunohistochemistry (Frozen sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] (ab308306)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse skeletal muscle (fresh) tissue labeling Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 with ab308306 at 1/100 dilution (5.04 ug/ml) followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/mL) (Green).

Confocal image showing positive staining on mouse skeletal muscle.

The nuclear counterstain was DAPI (Blue). The section was incubated with ab308306 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).

Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/mL).
Immunohistochemistry (Frozen sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] (ab308306)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse kidney (fresh) tissue labeling Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 with ab308306 at 1/100 dilution (5.04 ug/ml) followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/mL) (Green).

Low expression: confocal image showing no staining on mouse kidney.

The nuclear counterstain was DAPI (Blue). The section was incubated with ab308306 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).

Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/mL).
Immunohistochemistry (Frozen sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] (ab308306)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse testis (fresh) tissue labeling Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 with ab308306 at 1/100 dilution (5.04 ug/ml) followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/mL) (Green).

Low expression: confocal image showing no staining on mouse testis.

The nuclear counterstain was DAPI (Blue). The section was incubated with ab308306 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).

Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/mL).
Immunohistochemistry (Frozen sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] (ab308306)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat skeletal muscle (fresh) tissue labeling Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 with ab308306 at 1/100 dilution (5.04 ug/ml) followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/mL) (Green).

Confocal image showing positive staining on rat skeletal muscle.

The nuclear counterstain was DAPI (Blue). The section was incubated with ab308306 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).

Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/mL).
Immunohistochemistry (Frozen sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] (ab308306)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat kidney (fresh) tissue labeling Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 with ab308306 at 1/100 dilution (5.04 ug/ml) followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/mL) (Green).

Low expression: confocal image showing no staining on rat kidney.

The nuclear counterstain was DAPI (Blue). The section was incubated with ab308306 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).

Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/mL).
Immunohistochemistry (Frozen sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] (ab308306)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat testis (fresh) tissue labeling Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 with ab308306 at 1/100 dilution (5.04 ug/ml) followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/mL) (Green).

Low expression: confocal image showing no staining on rat testis.

The nuclear counterstain was DAPI (Blue). The section was incubated with ab308306 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).

Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/mL).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] (ab308306)

Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 with ab308306 at 1/500 dilution (1.008 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

Cytoplasmic staining on human skeletal muscle (PMID:34228850).

The section was incubated with ab308306 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] (ab308306)

Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue labeling Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 with ab308306 at 1/2000 dilution (0.252 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

Cytoplasmic staining on mouse skeletal muscle (PMID: 30089464).

The section was incubated with ab308306 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] (ab308306)

Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue labeling Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 with ab308306 at 1/2000 dilution (0.252 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

Cytoplasmic staining on rat skeletal muscle.

The section was incubated with ab308306 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] (ab308306)

Immunohistochemical analysis of paraffin-embedded cell pellets labeling Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 with ab308306 at 1/2000 dilution (0.252 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

Positive staining on (A) HEK-293T transfected with a CHRNA1 his tag construct, no staining on (B) HEK-293T transfected with empty plasmid.

The section was incubated with ab308306 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] (ab308306)

Immunohistochemical analysis of paraffin-embedded human testis tissue labeling Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 with ab308306 at 1/500 dilution (1.008 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

Negative control: no staining on human testis.

The section was incubated with ab308306 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] (ab308306)

Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 with ab308306 at 1/500 dilution (1.008 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

Negative control: no staining on human kidney.

The section was incubated with ab308306 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] (ab308306)

Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 with ab308306 at 1/2000 dilution (0.252 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

Negative control: no staining on mouse testis.

The section was incubated with ab308306 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] (ab308306)

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 with ab308306 at 1/2000 dilution (0.252 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

Negative control: no staining on mouse kidney.

The section was incubated with ab308306 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] (ab308306)

Immunohistochemical analysis of paraffin-embedded rat testis tissue labeling Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 with ab308306 at 1/2000 dilution (0.252 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

Negative control: no staining on rat testis.

The section was incubated with ab308306 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] (ab308306)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

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Publishing research using ab308306? Please let us know so that we can cite the reference in this datasheet.

ab308306 has not yet been referenced specifically in any publications.

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Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Common marmoset Tissue sections (Cochlea)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH6.0

Permeabilization

Specification

Cochlea

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C

Fixative

Paraformaldehyde

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Submitted Aug 15 2023

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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Related Products

Applications

The Abpromise guarantee

Target

Function

Tissue specificity

Involvement in disease

Sequence similarities

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

Certificate of Compliance

References (0)

Customer reviews and Q&As

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4]