Recombinant Anti-Niemann Pick C1 Like 1/NPC1L1 antibody [EPR5717] (ab124801)
![Western blot - Anti-Niemann Pick C1 Like 1/NPC1L1 antibody [EPR5717] (ab124801)](https://www.abcam.com/ps/products/124/ab124801/Images/ab124801-257155-anti-niemann-pick-c1-like-1-antibody-epr5717-western-blot.jpg "Western blot - Anti-Niemann Pick C1 Like 1/NPC1L1 antibody [EPR5717] (ab124801)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5717] to Niemann Pick C1 Like 1/NPC1L1
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Niemann Pick C1 Like 1/NPC1L1 antibody [EPR5717]
See all Niemann Pick C1 Like 1/NPC1L1 primary antibodies -
Description
Rabbit monoclonal [EPR5717] to Niemann Pick C1 Like 1/NPC1L1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human fetal liver and HeLa lysates; Human liver tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5717 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124801 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 149 kDa (predicted molecular weight: 149 kDa).
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| Notes |
|---|
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WB
1/1000 - 1/10000. Detects a band of approximately 149 kDa (predicted molecular weight: 149 kDa). |
Application notes
Is unsuitable for ICC/IF or IHC-P.
Target
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Function
Play a major role in cholesterol homeostasis. Is critical for the uptake of cholesterol across the plasma membrane of the intestinal enterocyte. Is the direct molecular target of ezetimibe, a drug that inhibits cholesterol absorbtion. Lack of activity leads to multiple lipid transport defects. The protein may have a function in the transport of multiple lipids and their homeostasis, and may play a critical role in regulating lipid metabolism. -
Tissue specificity
Widely expressed. Expressed in liver. Also expressed in small intestine, pancreas, kidney, lung, pancreas, spleen, heart, gall bladder, brain, testis, stomach and muscle. -
Sequence similarities
Belongs to the patched family.
Contains 1 SSD (sterol-sensing) domain. -
Post-translational
modificationsHighly glycosylated. -
Cellular localization
Apical cell membrane. Cell membrane. Cytoplasmic vesicle membrane. Subfractionation of brush border membranes from proximal enterocytes suggests considerable association with the apical membrane fraction. Exists as a predominantly cell surface membrane expressed protein (By similarity). According to PubMed:15671032, localizes in a subcellular vesicular compartment rich in RAB5. - Information by UniProt
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Database links
- Entrez Gene: 29881 Human
- Omim: 608010 Human
- SwissProt: Q9UHC9 Human
- Unigene: 567486 Human
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Alternative names
- Niemann Pick C1 like protein 1 antibody
- Niemann Pick disease type C1 gene like 1 antibody
- Niemann-Pick C1-like protein 1 antibody
see all
Images
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Western blot - Anti-Niemann Pick C1 Like 1/NPC1L1 antibody [EPR5717] (ab124801)Anti-Niemann Pick C1 Like 1/NPC1L1 antibody [EPR5717] (ab124801) at 1/1000 dilution (purified) + HeLa whole cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 149 kDa
Observed band size: 149 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
![Western blot - Anti-Niemann Pick C1 Like 1/NPC1L1 antibody [EPR5717] (ab124801)](https://www.abcam.com/ps/products/124/ab124801/Images/ab124801-257154-anti-niemann-pick-c1-like-1-antibody-epr5717-western-blot.jpg)
Western blot - Anti-Niemann Pick C1 Like 1/NPC1L1 antibody [EPR5717] (ab124801)Anti-Niemann Pick C1 Like 1/NPC1L1 antibody [EPR5717] (ab124801) at 1/10000 dilution (purified) + Human fetal liver tissue lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 149 kDa
Observed band size: 149 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
![Western blot - Anti-Niemann Pick C1 Like 1/NPC1L1 antibody [EPR5717] (ab124801)](https://www.abcam.com/ps/products/124/ab124801/Images/ab124801-138698-anti-niemann-pick-c1-like-1-antibody-epr5717-western-blot.jpg)
Western blot - Anti-Niemann Pick C1 Like 1/NPC1L1 antibody [EPR5717] (ab124801)All lanes : Anti-Niemann Pick C1 Like 1/NPC1L1 antibody [EPR5717] (ab124801) at 1/1000 dilution (unpurified)
Lane 1 : Human fetal liver lysates
Lane 2 : HeLa lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 149 kDa -
![Anti-Niemann Pick C1 Like 1/NPC1L1 antibody [EPR5717] (ab124801)](https://www.abcam.com/ps/products/124/ab124801/Images/ab124801-5-benefits-of-recombinant-antibodies.png)
Anti-Niemann Pick C1 Like 1/NPC1L1 antibody [EPR5717] (ab124801)
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab124801 has been referenced in 8 publications.
- Hong J et al. The Effects of Anthocyanin-Rich Bilberry Extract on Transintestinal Cholesterol Excretion. Foods 10:N/A (2021). PubMed: 34829135
- Chen X et al. Interaction between Flavonoids and Carotenoids on Ameliorating Oxidative Stress and Cellular Uptake in Different Cells. Foods 10:N/A (2021). PubMed: 34945647
- Zang R et al. Cholesterol 25-hydroxylase suppresses SARS-CoV-2 replication by blocking membrane fusion. Proc Natl Acad Sci U S A 117:32105-32113 (2020). PubMed: 33239446
- Wang Y et al. Hepatic NPC1L1 overexpression attenuates alcoholic autophagy in mice. Mol Med Rep 20:3224-3232 (2019). PubMed: 31432115
- Yang F et al. Fatty acids modulate the expression levels of key proteins for cholesterol absorption in Caco-2 monolayer. Lipids Health Dis 17:32 (2018). PubMed: 29463265
- Yang F et al. Egg-Yolk Sphingomyelin and Phosphatidylcholine Attenuate Cholesterol Absorption in Caco-2 Cells. Lipids 53:217-233 (2018). PubMed: 29569242
- Hu YW et al. A lincRNA-DYNLRB2-2/GPR119/GLP-1R/ABCA1-dependent signal transduction pathway is essential for the regulation of cholesterol homeostasis. J Lipid Res 55:681-97 (2014). PubMed: 24493833
- Renner O et al. Role of the ABCG8 19H risk allele in cholesterol absorption and gallstone disease. BMC Gastroenterol 13:30 (2013). WB . PubMed: 23406058