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    products/primary-antibodies/nkx22-antibody-epr14638-n-terminal-ab191077.pdf

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Neuroscience Neurology process Growth and Development Axonal Guidance Proteins
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RecombinantRabMAb

Recombinant Anti-Nkx2.2 antibody [EPR14638] - N-terminal (ab191077)

  • Datasheet
  • SDS
  • Certificate of Compliance
Reviews (4) Submit a question References (5)

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Immunocytochemistry/ Immunofluorescence - Anti-Nkx2.2 antibody [EPR14638] - N-terminal (ab191077)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody [EPR14638] - N-terminal (ab191077)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody [EPR14638] - N-terminal (ab191077)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody [EPR14638] - N-terminal (ab191077)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody [EPR14638] - N-terminal (ab191077)
  • Anti-Nkx2.2 antibody [EPR14638] - N-terminal (ab191077)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14638] to Nkx2.2 - N-terminal
  • Suitable for: IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-Nkx2.2 antibody [EPR14638] - N-terminal
    See all Nkx2.2 primary antibodies
  • Description

    Rabbit monoclonal [EPR14638] to Nkx2.2 - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human Ewing sarcoma, Human colon, Mouse brain and Rat colon tissues; C6 cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR14638
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Growth and Development
    • Axonal Guidance Proteins
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Developmental Families
    • Other
    • Stem Cells
    • Lineage Markers
    • Ectoderm
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors
    • Developmental Biology
    • Organogenesis
    • Gut development
    • Pancreas development

Associated products

  • Alternative Versions

    • Alexa Fluor® 488 Anti-Nkx2.2 antibody [EPR14638] - N-terminal (ab210463)
    • Anti-Nkx2.2 antibody [EPR14638] - BSA and Azide free (ab240318)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab191077 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P (1)
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF (2)
1/50.
Notes
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/50.

Target

  • Function

    May be involved in specifying diencephalic neuromeric boundaries, and in controlling the expression of genes that play a role in axonal guidance.
  • Sequence similarities

    Belongs to the NK-2 homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Domain

    The homeodomain is essential for interaction with OLIG2.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession O95096 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 4821 Human
    • Entrez Gene: 18088 Mouse
    • Entrez Gene: 366214 Rat
    • Omim: 604612 Human
    • SwissProt: O95096 Human
    • SwissProt: P42586 Mouse
    • Unigene: 516922 Human
    • Unigene: 330639 Mouse
    • Alternative names

      • Homeobox protein NK 2 homolog B antibody
      • Homeobox protein NK-2 homolog B antibody
      • Homeobox protein Nkx 2.2 antibody
      • Homeobox protein Nkx-2.2 antibody
      • NK 2 homolog B antibody
      • NK2 homeobox 2 antibody
      • NK2 transcription factor like protein B antibody
      • NK2 transcription factor related locus 2 antibody
      • NKX2 2 antibody
      • NKX2-2 antibody
      • NKX22 antibody
      • NKX22_HUMAN antibody
      • Nkx2b antibody
      • tinman antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-Nkx2.2 antibody [EPR14638] - N-terminal (ab191077)
      Immunocytochemistry/ Immunofluorescence - Anti-Nkx2.2 antibody [EPR14638] - N-terminal (ab191077)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed C6 cells labeling Nkx2.2 with ab191077 at 1/50, Goat anti rabbit IgG (Alexa Fluor® 555) at 1/200 and DAPI staining (blue). Negative controls also shown.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody [EPR14638] - N-terminal (ab191077)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody [EPR14638] - N-terminal (ab191077)

      Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling Nkx2.2 with ab191077 at 1/1000. Secondary antibody Goat anti rabbit IgG (HRP) prediluted. Counter stain Hematoxylin. Negative control also shown.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody [EPR14638] - N-terminal (ab191077)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody [EPR14638] - N-terminal (ab191077)

      Immunohistochemical analysis of paraffin-embedded Mouse brain tissue labeling Nkx2.2 with ab191077 at 1/1000. Secondary antibody Goat anti rabbit IgG (HRP) prediluted. Counter stain Hematoxylin. Negative control also shown.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody [EPR14638] - N-terminal (ab191077)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody [EPR14638] - N-terminal (ab191077)

      Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Nkx2.2 with ab191077 at 1/1000. Secondary antibody Goat anti rabbit IgG (HRP) prediluted. Counter stain Hematoxylin. Negative control also shown.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody [EPR14638] - N-terminal (ab191077)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody [EPR14638] - N-terminal (ab191077)

      Immunohistochemical analysis of paraffin-embedded Human Ewing sarcoma tissue labeling Nkx2.2 with ab191077 at 1/1000. Secondary antibody Goat anti rabbit IgG (HRP) prediluted. Counter stain Hematoxylin. Negative control also shown.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Anti-Nkx2.2 antibody [EPR14638] - N-terminal (ab191077)
      Anti-Nkx2.2 antibody [EPR14638] - N-terminal (ab191077)

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (5)

    Publishing research using ab191077? Please let us know so that we can cite the reference in this datasheet.

    ab191077 has been referenced in 5 publications.

    • Crane-Smith Z  et al. A Highly Conserved Shh Enhancer Coordinates Hypothalamic and Craniofacial Development. Front Cell Dev Biol 9:595744 (2021). PubMed: 33869166
    • Zhang S  et al. Single-cell transcriptomics identifies divergent developmental lineage trajectories during human pituitary development. Nat Commun 11:5275 (2020). PubMed: 33077725
    • Hoveizi E & Mohammadi T Differentiation of endometrial stem cells into insulin-producing cells using signaling molecules and zinc oxide nanoparticles, and three-dimensional culture on nanofibrous scaffolds. J Mater Sci Mater Med 30:101 (2019). PubMed: 31473826
    • Bradley RA  et al. Regionally specified human pluripotent stem cell-derived astrocytes exhibit different molecular signatures and functional properties. Development 146:N/A (2019). PubMed: 31189664
    • Lam CJ  et al. Highly Proliferative a-Cell-Related Islet Endocrine Cells in Human Pancreata. Diabetes 67:674-686 (2018). PubMed: 29326366

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-4 of 4 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Nkx2.2 antibody [EPR14638] - N-terminal

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (embryonic mouse brain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: DAKO antigen retrieval solution
    Permeabilization
    Yes - PBS + 0.3% Triton X-100
    Specification
    embryonic mouse brain
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 21°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Laura Lahti

    Verified customer

    Submitted Dec 03 2021

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Nkx2.2 antibody [EPR14638] - N-terminal

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (embryonic)
    Permeabilization
    Yes - Tritonx100
    Specification
    embryonic
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jun 30 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Nkx2.2 antibody [EPR14638] - N-terminal

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (neural tube of mouse embryos)
    Permeabilization
    Yes - 0.1% Triton X-100
    Specification
    neural tube of mouse embryos
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Oct 31 2019

    Immunohistochemistry (Frozen sections) abreview for Anti-Nkx2.2 antibody [EPR14638] - N-terminal

    Excellent
    Abreviews
    Abreviews
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (embryo/ adult brain)
    Permeabilization
    Yes - 0.3% triton in PBS
    Specification
    embryo/ adult brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 23°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. steven wyler

    Verified customer

    Submitted Sep 24 2015

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