Anti-Nkx2.2 antibody [NX2/294] (ab187375)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody [NX2/294] (ab187375)](https://www.abcam.com/ps/products/187/ab187375/Images/ab187375-395234-anti-nkx2-2-antibody-nx2-294-immunohistochemistry-evings-sarcoma-human.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody [NX2/294] (ab187375)")
Key features and details
- Mouse monoclonal [NX2/294] to Nkx2.2
- Suitable for: IHC-P
- Reacts with: Rat, Human
- Isotype: IgG2b
Overview
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Product name
Anti-Nkx2.2 antibody [NX2/294]
See all Nkx2.2 primary antibodies -
Description
Mouse monoclonal [NX2/294] to Nkx2.2 -
Host species
Mouse -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Recombinant full length protein corresponding to Human Nkx2.2 aa 1-273.
Database link: O95096 -
Positive control
- IHC-P: Human and rat pancreas and Human Ewing sarcoma tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A/G purified -
Clonality
Monoclonal -
Clone number
NX2/294 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab187375 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | (1) |
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Performed for 30 minutes at RT Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes |
| Notes |
|---|
|
IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. Performed for 30 minutes at RT Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes |
Target
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Function
May be involved in specifying diencephalic neuromeric boundaries, and in controlling the expression of genes that play a role in axonal guidance. -
Sequence similarities
Belongs to the NK-2 homeobox family.
Contains 1 homeobox DNA-binding domain. -
Domain
The homeodomain is essential for interaction with OLIG2. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 4821 Human
- Entrez Gene: 366214 Rat
- Omim: 604612 Human
- SwissProt: O95096 Human
- Unigene: 516922 Human
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Alternative names
- Homeobox protein NK 2 homolog B antibody
- Homeobox protein NK-2 homolog B antibody
- Homeobox protein Nkx 2.2 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody [NX2/294] (ab187375)
Immunohistochemical analysis of paraffin embedded human Ewing's Sarcoma tissue labeling Nkx2.2 with ab187375 at 2 µg/mL.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody [NX2/294] (ab187375)](https://www.abcam.com/ps/products/187/ab187375/Images/ab187375-395233-anti-nkx2-2-antibody-nx2-294-immunohistochemistry-pancreas-rat.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody [NX2/294] (ab187375)Immunohistochemical analysis of paraffin embedded rat pancreas tissue labeling Nkx2.2 with ab187375 at 2 µg/mL.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody [NX2/294] (ab187375)](https://www.abcam.com/ps/products/187/ab187375/Images/ab187375-219568-anti-nkx22-antibody-nx2-294-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody [NX2/294] (ab187375)Immunohistochemical analysis of paraffin embedded Human pancreas tissue labeling Nkx2.2 with ab187375 at 2 µg/mL.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab187375 has been referenced in 3 publications.
- Ross NT et al. CPSF3-dependent pre-mRNA processing as a druggable node in AML and Ewing's sarcoma. Nat Chem Biol 16:50-59 (2020). PubMed: 31819276
- Nabet B et al. Rapid and direct control of target protein levels with VHL-recruiting dTAG molecules. Nat Commun 11:4687 (2020). PubMed: 32948771
- Huang W et al. Origins and Proliferative States of Human Oligodendrocyte Precursor Cells. Cell 182:594-608.e11 (2020). PubMed: 32679030