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  1. Link

    products/primary-antibodies/nkx22-antibody-nx2294-ab187375.pdf

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Neuroscience Neurology process Growth and Development Axonal Guidance Proteins
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Anti-Nkx2.2 antibody [NX2/294] (ab187375)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (3)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody [NX2/294] (ab187375)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody [NX2/294] (ab187375)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody [NX2/294] (ab187375)

Key features and details

  • Mouse monoclonal [NX2/294] to Nkx2.2
  • Suitable for: IHC-P
  • Reacts with: Rat, Human
  • Isotype: IgG2b

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Overview

  • Product name

    Anti-Nkx2.2 antibody [NX2/294]
    See all Nkx2.2 primary antibodies
  • Description

    Mouse monoclonal [NX2/294] to Nkx2.2
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Recombinant full length protein corresponding to Human Nkx2.2 aa 1-273.
    Database link: O95096

  • Positive control

    • IHC-P: Human and rat pancreas and Human Ewing sarcoma tissues.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.05% Sodium azide
    Constituents: 99% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A/G purified
  • Clonality

    Monoclonal
  • Clone number

    NX2/294
  • Isotype

    IgG2b
  • Light chain type

    kappa
  • Research areas

    • Neuroscience
    • Neurology process
    • Growth and Development
    • Axonal Guidance Proteins
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Developmental Families
    • Other
    • Stem Cells
    • Lineage Markers
    • Ectoderm
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors
    • Developmental Biology
    • Organogenesis
    • Gut development
    • Pancreas development

Associated products

  • Alternative Versions

    • Anti-Nkx2.2 antibody [NX2/294] - BSA and Azide free (ab212686)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control (ab170192)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab187375 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P (1)
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Performed for 30 minutes at RT

Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Notes
IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Performed for 30 minutes at RT

Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Target

  • Function

    May be involved in specifying diencephalic neuromeric boundaries, and in controlling the expression of genes that play a role in axonal guidance.
  • Sequence similarities

    Belongs to the NK-2 homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Domain

    The homeodomain is essential for interaction with OLIG2.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession O95096 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 4821 Human
    • Entrez Gene: 366214 Rat
    • Omim: 604612 Human
    • SwissProt: O95096 Human
    • Unigene: 516922 Human
    • Alternative names

      • Homeobox protein NK 2 homolog B antibody
      • Homeobox protein NK-2 homolog B antibody
      • Homeobox protein Nkx 2.2 antibody
      • Homeobox protein Nkx-2.2 antibody
      • NK 2 homolog B antibody
      • NK2 homeobox 2 antibody
      • NK2 transcription factor like protein B antibody
      • NK2 transcription factor related locus 2 antibody
      • NKX2 2 antibody
      • NKX2-2 antibody
      • NKX22 antibody
      • NKX22_HUMAN antibody
      • Nkx2b antibody
      • tinman antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody [NX2/294] (ab187375)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody [NX2/294] (ab187375)

      Immunohistochemical analysis of paraffin embedded human Ewing's Sarcoma tissue labeling Nkx2.2 with ab187375 at 2 µg/mL.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody [NX2/294] (ab187375)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody [NX2/294] (ab187375)

      Immunohistochemical analysis of paraffin embedded rat pancreas tissue labeling Nkx2.2 with ab187375 at 2 µg/mL.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody [NX2/294] (ab187375)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody [NX2/294] (ab187375)

      Immunohistochemical analysis of paraffin embedded Human pancreas tissue labeling Nkx2.2 with ab187375 at 2 µg/mL.

    Protocols

    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (3)

    Publishing research using ab187375? Please let us know so that we can cite the reference in this datasheet.

    ab187375 has been referenced in 3 publications.

    • Ross NT  et al. CPSF3-dependent pre-mRNA processing as a druggable node in AML and Ewing's sarcoma. Nat Chem Biol 16:50-59 (2020). PubMed: 31819276
    • Nabet B  et al. Rapid and direct control of target protein levels with VHL-recruiting dTAG molecules. Nat Commun 11:4687 (2020). PubMed: 32948771
    • Huang W  et al. Origins and Proliferative States of Human Oligodendrocyte Precursor Cells. Cell 182:594-608.e11 (2020). PubMed: 32679030

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Nkx2.2 antibody [NX2/294]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Ewing's Sarcoma)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: EDTA, pH9, 100C, 20 min
    Permeabilization
    No
    Specification
    Ewing's Sarcoma
    Blocking step
    H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Oct 05 2015

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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