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RecombinantRabMAb

Recombinant Anti-nkx6.1 antibody [EPR20328] (ab221544)

Submit a review Submit a question References (1)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20328] (ab221544)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20328] (ab221544)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20328] (ab221544)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20328] (ab221544)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20328] (ab221544)
  • Western blot - Anti-nkx6.1 antibody [EPR20328] (ab221544)
  • Western blot - Anti-nkx6.1 antibody [EPR20328] (ab221544)
  • Anti-nkx6.1 antibody [EPR20328] (ab221544)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20328] to nkx6.1
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-nkx6.1 antibody [EPR20328]
    See all nkx6.1 primary antibodies

  • Description

    Rabbit monoclonal [EPR20328] to nkx6.1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human nkx6.1 recombinant protein (aa (1-48) & aa(66-120) & aa(179-240) with a His-tag). IHC-P: Human pancreas and pancreatic neuroendocrine neoplasm tissues; Mouse and rat pancreas tissues.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab221544 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000. Predicted molecular weight: 38 kDa.

Detection of endogenous expression of nkx6.1 in WB will require optimization. Under our experimental conditions we only detected weak endogenous expression in mouse lysates.
IHC-P
1/200. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
WB
1/1000. Predicted molecular weight: 38 kDa.

Detection of endogenous expression of nkx6.1 in WB will require optimization. Under our experimental conditions we only detected weak endogenous expression in mouse lysates.
IHC-P
1/200. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Transcription factor which binds to specific A/T-rich DNA sequences in the promoter regions of a number of genes. Involved in transcriptional regulation in islet beta cells. Binds to the insulin promoter and is involved in regulation of the insulin gene. Together with NKX2-2 and IRX3 acts to restrict the generation of motor neurons to the appropriate region of the neural tube. Belongs to the class II proteins of neuronal progenitor factors, which are induced by SHH signals.

  • Tissue specificity

    Pancreatic beta cells.

  • Sequence similarities

    Contains 1 homeobox DNA-binding domain.

  • Domain

    The C-terminal domain contributes to sequence-specific DNA-binding.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Homeobox protein NK-6 homolog A antibody
    • Homeobox protein Nkx-6.1 antibody
    • NK homeo box, family 6, member A antibody
    • NK homeobox (Drosophila), family 6, A antibody
    • NK homeobox, family 6, A antibody
    • NK6 homeobox 1 antibody
    • NK6 transcription factor homolog A antibody
    • NK6 transcription factor related, locus 1 antibody
    • NK6 transcription factor related, locus 1 (Drosophila) antibody
    • NKX6-1 antibody
    • NKX6.1 antibody
    • NKX61 antibody
    • NKX61_HUMAN antibody
    • NKX6A antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20328] (ab221544)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20328] (ab221544)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling nkx6.1 with ab221544 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on islet of human pancreas (PMID: 8702531). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20328] (ab221544)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20328] (ab221544)

    Immunohistochemical analysis of paraffin-embedded human pancreatic neuroendocrine neoplasm tissue labeling nkx6.1 with ab221544 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Mainly nuclear staining on tumor cells of human pancreatic neuroendocrine neoplasm (PMID: 25871618). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20328] (ab221544)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20328] (ab221544)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue labeling nkx6.1 with ab221544 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on islet of mouse pancreas (PMID: 8702531). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20328] (ab221544)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20328] (ab221544)

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue labeling nkx6.1 with ab221544 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on rat pancreas (PMID: 8702531). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20328] (ab221544)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20328] (ab221544)

    Immunohistochemical analysis of paraffin-embedded human colon tissue labeling nkx6.1 with ab221544 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Negative control: no staining on human colon (PMID: 25871618).

    Counter stained with hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-nkx6.1 antibody [EPR20328] (ab221544)
    Western blot - Anti-nkx6.1 antibody [EPR20328] (ab221544)
    Anti-nkx6.1 antibody [EPR20328] (ab221544) at 1/1000 dilution + Human nkx6.1 recombinant protein (aa (1-48) & aa(66-120) & aa(179-240) with a His-tag) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 38 kDa
    Observed band size: 40 kDa why is the actual band size different from the predicted?


    Exposure time: 3 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Human nkx6.1 recombinant protein contains aa(1-48) & aa(66-120) & aa(179-240) with a His-tag.

  • Western blot - Anti-nkx6.1 antibody [EPR20328] (ab221544)
    Western blot - Anti-nkx6.1 antibody [EPR20328] (ab221544)
    Anti-nkx6.1 antibody [EPR20328] (ab221544) at 1/200 dilution + Beta-TC-6 (mouse pancreas insulinoma beta cell) whole cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 38 kDa
    Observed band size: 38 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-nkx6.1 antibody [EPR20328] (ab221544)
    Anti-nkx6.1 antibody [EPR20328] (ab221544)

References (1)

Publishing research using ab221544? Please let us know so that we can cite the reference in this datasheet.

ab221544 has been referenced in 1 publication.

  • Weng Q  et al. STAT3 dictates ß-cell apoptosis by modulating PTEN in streptozocin-induced hyperglycemia. Cell Death Differ 27:130-145 (2020). PubMed: 31097787

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