Recombinant Anti-nkx6.1 antibody [EPR20328] - BSA and Azide free (ab251565)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20328] to nkx6.1 - BSA and Azide free
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-nkx6.1 antibody [EPR20328] - BSA and Azide free
See all nkx6.1 primary antibodies -
Description
Rabbit monoclonal [EPR20328] to nkx6.1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab251565 is the carrier-free version of ab221544.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20328 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab251565 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 38 kDa.
Detection of endogenous expression of nkx6.1 in WB will require optimization. Under our experimental conditions we only detected weak endogenous expression in mouse lysates. |
Notes |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 38 kDa. Detection of endogenous expression of nkx6.1 in WB will require optimization. Under our experimental conditions we only detected weak endogenous expression in mouse lysates. |
Target
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Function
Transcription factor which binds to specific A/T-rich DNA sequences in the promoter regions of a number of genes. Involved in transcriptional regulation in islet beta cells. Binds to the insulin promoter and is involved in regulation of the insulin gene. Together with NKX2-2 and IRX3 acts to restrict the generation of motor neurons to the appropriate region of the neural tube. Belongs to the class II proteins of neuronal progenitor factors, which are induced by SHH signals. -
Tissue specificity
Pancreatic beta cells. -
Sequence similarities
Contains 1 homeobox DNA-binding domain. -
Domain
The C-terminal domain contributes to sequence-specific DNA-binding. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 4825 Human
- Entrez Gene: 18096 Mouse
- Entrez Gene: 65193 Rat
- Omim: 602563 Human
- SwissProt: P78426 Human
- SwissProt: Q99MA9 Mouse
- SwissProt: O35762 Rat
- Unigene: 546270 Human
see all -
Alternative names
- Homeobox protein NK-6 homolog A antibody
- Homeobox protein Nkx-6.1 antibody
- NK homeo box, family 6, member A antibody
see all
Images
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This data was developed using ab221544, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling nkx6.1 with ab221544 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on islet of human pancreas (PMID: 8702531). Counter stained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. -
This data was developed using ab221544, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human pancreatic neuroendocrine neoplasm tissue labeling nkx6.1 with ab221544 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Mainly nuclear staining on tumor cells of human pancreatic neuroendocrine neoplasm (PMID: 25871618). Counter stained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. -
This data was developed using ab221544, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue labeling nkx6.1 with ab221544 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on islet of mouse pancreas (PMID: 8702531). Counter stained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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This data was developed using ab221544, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded rat pancreas tissue labeling nkx6.1 with ab221544 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on rat pancreas (PMID: 8702531). Counter stained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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This data was developed using ab221544, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human colon tissue labeling nkx6.1 with ab221544 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Negative control: no staining on human colon (PMID: 25871618). Counter stained with hematoxylin. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Anti-nkx6.1 antibody [EPR20328] (ab221544) at 1/1000 dilution + Human nkx6.1 recombinant protein (aa (1-48) & aa(66-120) & aa(179-240) with a His-tag) at 0.01 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 38 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
Exposure time: 3 secondsThis data was developed using ab221544, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Human nkx6.1 recombinant protein contains aa1-48, aa66-120, and aa179-240 with a His-tag.
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Anti-nkx6.1 antibody [EPR20328] (ab221544) at 1/200 dilution + Beta-TC-6 (mouse pancreas insulinoma beta cell) whole cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 38 kDa
Observed band size: 38 kDa
Exposure time: 3 minutesThis data was developed using ab221544, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (1)
ab251565 has been referenced in 1 publication.
- Zhang S et al. Porcine pancreas mesenchymal cell characterization and functional differentiation into insulin-producing cells in vitro. Mol Med Rep 24:N/A (2021). PubMed: 34414446