Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
RecombinantRabMAb

Recombinant Anti-nkx6.1 antibody [EPR20405] (ab221549)

Reviews (1) Submit a question References (5)
Immunohistochemistry (Frozen sections) - Anti-nkx6.1 antibody [EPR20405] (ab221549)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20405] (ab221549)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20405] (ab221549)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20405] (ab221549)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20405] (ab221549)
  • Western blot - Anti-nkx6.1 antibody [EPR20405] (ab221549)
  • Western blot - Anti-nkx6.1 antibody [EPR20405] (ab221549)
  • Anti-nkx6.1 antibody [EPR20405] (ab221549)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20405] to nkx6.1
  • Suitable for: IHC-Fr, WB, IHC-P
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-nkx6.1 antibody [EPR20405]
    See all nkx6.1 primary antibodies

  • Description

    Rabbit monoclonal [EPR20405] to nkx6.1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-Fr, WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human fetal pancreas lysate. Beta-TC-6 whole cell lysate. IHC-P: Human pancreas and pancreatic neuroendocrine neoplasm tissues; Mouse pancreas tissue. IHC-Fr: Mouse pancreas tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab221549 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr
1/100.
WB
1/1000. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).

Under our testing conditions we used a high sensitivity exposure system to obtain this result. Customers are recommended to optimize WB testing conditions.
IHC-P (1)
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
IHC-Fr
1/100.
WB
1/1000. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).

Under our testing conditions we used a high sensitivity exposure system to obtain this result. Customers are recommended to optimize WB testing conditions.
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Transcription factor which binds to specific A/T-rich DNA sequences in the promoter regions of a number of genes. Involved in transcriptional regulation in islet beta cells. Binds to the insulin promoter and is involved in regulation of the insulin gene. Together with NKX2-2 and IRX3 acts to restrict the generation of motor neurons to the appropriate region of the neural tube. Belongs to the class II proteins of neuronal progenitor factors, which are induced by SHH signals.

  • Tissue specificity

    Pancreatic beta cells.

  • Sequence similarities

    Contains 1 homeobox DNA-binding domain.

  • Domain

    The C-terminal domain contributes to sequence-specific DNA-binding.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • Homeobox protein NK-6 homolog A antibody
      • Homeobox protein Nkx-6.1 antibody
      • NK homeo box, family 6, member A antibody
      • NK homeobox (Drosophila), family 6, A antibody
      • NK homeobox, family 6, A antibody
      • NK6 homeobox 1 antibody
      • NK6 transcription factor homolog A antibody
      • NK6 transcription factor related, locus 1 antibody
      • NK6 transcription factor related, locus 1 (Drosophila) antibody
      • NKX6-1 antibody
      • NKX6.1 antibody
      • NKX61 antibody
      • NKX61_HUMAN antibody
      • NKX6A antibody
      see all

    Images

    • Immunohistochemistry (Frozen sections) - Anti-nkx6.1 antibody [EPR20405] (ab221549)
      Immunohistochemistry (Frozen sections) - Anti-nkx6.1 antibody [EPR20405] (ab221549)

      Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse pancreas tissue labeling nkx6.1 with ab221549  at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Nuclear staining on islet of mouse pancreas is observed.

      The nuclear counterstain is DAPI (blue).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20405] (ab221549)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20405] (ab221549)

      Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling nkx6.1 with ab221549 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on islet of human pancreas (PMID: 8702531). Counter stained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20405] (ab221549)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20405] (ab221549)

      Immunohistochemical analysis of paraffin-embedded human pancreatic neuroendocrine neoplasm tissue labeling nkx6.1 with ab221549 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Mainly nuclear staining on tumor cells of human pancreatic neuroendocrine neoplasm (PMID: 25871618). Counter stained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20405] (ab221549)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20405] (ab221549)

      Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue labeling nkx6.1 with ab221549 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on islet of mouse pancreas (PMID: 8702531). Counter stained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20405] (ab221549)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20405] (ab221549)

      Immunohistochemical analysis of paraffin-embedded human colon tissue labeling nkx6.1 with ab221549 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      Negative control: no staining on human colon (PMID: 25871618).

      Counter stained with hematoxylin.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Western blot - Anti-nkx6.1 antibody [EPR20405] (ab221549)
      Western blot - Anti-nkx6.1 antibody [EPR20405] (ab221549)
      Anti-nkx6.1 antibody [EPR20405] (ab221549) at 1/1000 dilution + Human fetal pancreas lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Developed using the ECL technique.

      Predicted band size: 38 kDa
      Observed band size: 38 kDa


      Exposure time: 3 minutes


      Blocking/Dilution buffer: 5% NFDM/TBST.

      Under our testing conditions we used a high sensitivity exposure system to obtain this result. Customers are recommended to optimize WB testing conditions.    

    • Western blot - Anti-nkx6.1 antibody [EPR20405] (ab221549)
      Western blot - Anti-nkx6.1 antibody [EPR20405] (ab221549)
      Anti-nkx6.1 antibody [EPR20405] (ab221549) at 1/1000 dilution + Beta-TC-6 (mouse pancreas insulinoma beta cell) whole cell lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 38 kDa


      Exposure time: 30 seconds


      Blocking/Dilution buffer: 5% NFDM/TBST

    • Anti-nkx6.1 antibody [EPR20405] (ab221549)
      Anti-nkx6.1 antibody [EPR20405] (ab221549)

    References (5)

    Publishing research using ab221549? Please let us know so that we can cite the reference in this datasheet.

    ab221549 has been referenced in 5 publications.

    • Crane-Smith Z  et al. A Highly Conserved Shh Enhancer Coordinates Hypothalamic and Craniofacial Development. Front Cell Dev Biol 9:595744 (2021). PubMed: 33869166
    • Fernandes HJR  et al. Single-Cell Transcriptomics of Parkinson's Disease Human In Vitro Models Reveals Dopamine Neuron-Specific Stress Responses. Cell Rep 33:108263 (2020). PubMed: 33053338
    • Lin JY  et al. In vitro expansion of pancreatic islet clusters facilitated by hormones and chemicals. Cell Discov 6:20 (2020). PubMed: 32284878
    • Tokumoto S  et al. Generation and Characterization of a Novel Mouse Model That Allows Spatiotemporal Quantification of Pancreatic ß-Cell Proliferation. Diabetes 69:2340-2351 (2020). PubMed: 32769118
    • Wei R  et al. Antagonistic Glucagon Receptor Antibody Promotes a-Cell Proliferation and Increases ß-Cell Mass in Diabetic Mice. iScience 16:326-339 (2019). PubMed: 31203188

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-nkx6.1 antibody [EPR20405]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (embryonic mouse brain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 1x DAKO antigen retrieval buffer
    Permeabilization
    Yes - 0.3% Triton X-100 in PBS for 5 minutes
    Specification
    embryonic mouse brain
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 21°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Laura Lahti

    Verified customer

    Submitted Dec 03 2021

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com