Name: Anti-Notch1 antibody [EP1238Y]
Brand: RabMAb
SKU: ab52627
Rating: 5 (17 reviews)

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Western blot - Anti-Notch1 antibody [EP1238Y] (ab52627)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP1238Y] to Notch1
Suitable for: IHC-P, Flow Cyt (Intra), WB, ICC/IF
Knockout validated
Reacts with: Mouse, Human

Alexa Fluor® 647 APC Carrier Free HRP PE

Product name

Anti-Notch1 antibody [EP1238Y]
See all Notch1 primary antibodies
Description

Rabbit monoclonal [EP1238Y] to Notch1
Host species

Rabbit
Specificity

80% identities with Notch 2 and 81% with Notch 3
Tested applications

Suitable for: IHC-P, Flow Cyt (Intra), WB, ICC/IFmore details
Unsuitable for: IP
Species reactivity

Reacts with: Mouse, Human
Predicted to work with: Cow
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HeLa, HAP1, MOLT-4, HEK-293 cell lysate. Mouse brain lysate. IHC-P: Human brain tissue. ICC/IF: HeLa cells. Flow Cyt (intra): HeLa cells.
General notes
Notch 1 (encoded by the Notch 1 gene) is a member of the Notch family of receptors. When bound to a ligand, the Notch1 receptor sends signals critical for normal development of many tissues throughout the body. Notch1 signalling supports the determination and specialization of cells as well as playing a role in cell proliferation, differentiation, and apoptosis.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EP1238Y
Isotype

IgG
Research areas
- Neuroscience
- Development

Alternative Versions
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Conjugation kits
- PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
- APC Conjugation Kit - Lightning-Link® (ab201807)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
KO cell lines
- Human NOTCH1 knockout HeLa cell line (ab261762)
KO cell lysates
- Human NOTCH1 knockout HeLa cell lysate (ab257006)
Recombinant Protein
- Recombinant Human Notch1 protein (ab114178)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab52627 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
IHC-P	(9)	1/100 - 1/150. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See IHC antigen retrieval protocols.
Flow Cyt (Intra)		1/130 - 1/200. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB	(7)	1/1000 - 1/2000. Detects a band of approximately 125 kDa.
ICC/IF		1/100 - 1/150.

Notes
IHC-P 1/100 - 1/150. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See IHC antigen retrieval protocols.
Flow Cyt (Intra) 1/130 - 1/200. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB 1/1000 - 1/2000. Detects a band of approximately 125 kDa.
ICC/IF 1/100 - 1/150.

Application notes

Is unsuitable for IP.

Function

Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May be important for normal lymphocyte function. In altered form, may contribute to transformation or progression in some T-cell neoplasms. Involved in the maturation of both CD4+ and CD8+ cells in the thymus. May be important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, may function as a receptor for neuronal DNER and may be involved in the differentiation of Bergmann glia.
Tissue specificity

In fetal tissues most abundant in spleen, brain stem and lung. Also present in most adult tissues where it is found mainly in lymphoid tissues.
Involvement in disease

Defects in NOTCH1 are a cause of bicuspid aortic valve (BAV) [MIM:109730]. A common defect in the aortic valve in which two rather than three leaflets are present. It is often associated with aortic valve calcification and insufficiency. In extreme cases, the blood flow may be so restricted that the left ventricle fails to grow, resulting in hypoplastic left heart syndrome.
Sequence similarities

Belongs to the NOTCH family.
Contains 5 ANK repeats.
Contains 36 EGF-like domains.
Contains 3 LNR (Lin/Notch) repeats.
Post-translational
modifications

Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane.
Phosphorylated.
O-glycosylated on the EGF-like domains. Contains both O-linked fucose and O-linked glucose.
Ubiquitinated; undergoes 'Lys-29'-linked polyubiquitination catalyzed by ITCH.
Cellular localization

Cell membrane and Nucleus. Following proteolytical processing NICD is translocated to the nucleus.
Target information above from: UniProt accession P46531 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 767866 Cow
- Entrez Gene: 4851 Human
- Entrez Gene: 18128 Mouse
- Omim: 190198 Human
- SwissProt: P46531 Human
- SwissProt: Q01705 Mouse
- Unigene: 495473 Human
- Unigene: 290610 Mouse
Alternative names
- 9930111A19Rik antibody
- AOS5 antibody
- AOVD1 antibody
- hN1 antibody
- Lin-12 antibody
- LIN12 antibody
- Mis6 antibody
- Motch A antibody
- mT14 antibody
- Neurogenic locus notch homolog protein 1 antibody
- Neurogenic locus notch protein homolog antibody
- NICD antibody
- NOTC1_HUMAN antibody
- Notch 1 antibody
- Notch 1 intracellular domain antibody
- NOTCH antibody
- Notch gene homolog 1 (Drosophila) antibody
- Notch homolog 1, translocation-associated (Drosophila) antibody
- NOTCH, Drosophila, homolog of, 1 antibody
- notch1 antibody
- TAN1 antibody
- Translocation associated notch homolog antibody
- Translocation associated notch protein TAN 1 antibody
- Translocation-associated notch protein TAN-1 antibody
- xotch antibody
see all

Western blot - Anti-Notch1 antibody [EP1238Y] (ab52627)

All lanes : Anti-Notch1 antibody [EP1238Y] (ab52627) at 1/1000 dilution

Lane 1 : Wild-type HeLa cell lysate
Lane 2 : NOTCH1 knockout HeLa cell lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Lanes 1- 2: Merged signal (red and green). Green - ab52627 observed at 110 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

ab52627 was shown to react with Notch1 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab261762 (knockout cell lysate ab257006) was used. Wild-type HeLa and NOTCH1 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab52627 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Immunocytochemistry/ Immunofluorescence - Anti-Notch1 antibody [EP1238Y] (ab52627)

Immunofluorescent staining of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells fixed with 4% PFA using purified ab52627 at a dilution of 1/150.

An Alexa Fluor^® 555 goat anti-rabbit was used as the secondary and the sample was stained with DAPI. An Alexa Fluor^® 555 goat anti-rabbit was used at a dilution of 1/500 as the negative control and is shown in the bottom right hand panel.
Western blot - Anti-Notch1 antibody [EP1238Y] (ab52627)

All lanes : Anti-Notch1 antibody [EP1238Y] (ab52627) at 1 µg/ml

Lane 1 : Wild-type HAP1 cell lysate at 40 µg
Lane 2 : NOTCH1 knockout HAP1 cell lysate at 40 µg
Lane 3 : MOLT-4 cell lysate at 20 µg
Lane 4 : HepG2 cell lysate at 20 µg

Performed under reducing conditions.

Observed band size: 105 kDa why is the actual band size different from the predicted?

Lanes 1 - 4: Merged signal (red and green). Green - ab52627 observed at 105 kDa. Red - loading control, ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

ab52627 was shown to react with Notch1 in wild-type HAP1 cells in western blot. Loss of signal was observed when NOTCH1 knockout sample was used. Wild-type and NOTCH1 knockout HAP1 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk before incubation with ab52627 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at 1 µg/ml and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-Notch1 antibody [EP1238Y] (ab52627)

Anti-Notch1 antibody [EP1238Y] (ab52627) at 1/2000 dilution (Purified) + Mouse brain at 10 µg

Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution

Observed band size: 125 kDa why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-Notch1 antibody [EP1238Y] (ab52627)

Anti-Notch1 antibody [EP1238Y] (ab52627) at 1/2000 dilution (Purified) + HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate at 10 µg

Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution

Observed band size: 125 kDa why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Notch1 antibody [EP1238Y] (ab52627)Ernst et al PLoS One. 2018 Feb 12;13(2):e0192525. doi: 10.1371/journal.pone.0192525. eCollection 2018. Fig 5. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

Verification of gene expression array data by immunohistochemical analysis of Notch 1 expression in subcutaneous tumors and lung metastases from a human melanoma (MeWo) xenograft experiment in mice.

Immunohistochemical staining for Notch1 expression (ab52627, red) in subcutaneous tumors and lung metastases (both panels) of MeWo (Human malignant melanoma cell line) cells.

All scale bars: 50 μm.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Notch1 antibody [EP1238Y] (ab52627)

Immunohistochemical staining of paraffin-embedded human brain with purified ab52627 at a dilution of 1/150.

A prediluted HRP polymer for rabbit IgG was used as the secondary and the sample was stained with hematoxylin. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Notch1 antibody [EP1238Y] (ab52627)

Immunohistochemical staining of paraffin-embedded human brain with unpurified ab52627 at a dilution of 1/100.

A prediluted HRP polymer for rabbit IgG was used as the secondary and the sample was stained with hematoxylin. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-Notch1 antibody [EP1238Y] (ab52627)

Immunofluorescent staining of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells fixed with 4% PFA using unpurified ab52627 at a dilution of 1/100.

An Alexa Fluor^® 555 goat anti-rabbit was used as the secondary and the sample was stained with DAPI. An Alexa Fluor^® 555 goat anti-rabbit was used at a dilution of 1/500 as the negative control and is shown in the bottom right hand panel.
Flow Cytometry (Intracellular) - Anti-Notch1 antibody [EP1238Y] (ab52627)

Intracellular Flow Cytometry analysis of permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells (2% PFA, pink) withpurified ab52627 at a 1/200 dilution, or negative control rabbit monoclonal IgG (green). The secondary antibody was FITC goat anti-rabbit.
Anti-Notch1 antibody [EP1238Y] (ab52627)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

Publishing research using ab52627? Please let us know so that we can cite the reference in this datasheet.

ab52627 has been referenced in 140 publications.

Weng Y et al. miR-133b has protective effect on rats with acute lung injury caused by severe acute pancreatitis through targeting sp1 gene. Int J Clin Exp Pathol 14:86-96 (2021). PubMed: 33532026
Ni XQ et al. Inhibition of Notch1-mediated inflammation by intermedin protects against abdominal aortic aneurysm via PI3K/Akt signaling pathway. Aging (Albany NY) 13:5164-5184 (2021). PubMed: 33535178
Shao H et al. Converting melanoma-associated fibroblasts into a tumor-suppressive phenotype by increasing intracellular Notch1 pathway activity. PLoS One 16:e0248260 (2021). PubMed: 33705467
Feng Y et al. SLC6A8 is involved in the progression of non-small cell lung cancer through the Notch signaling pathway. Ann Transl Med 9:264 (2021). PubMed: 33708891
Xiang Q et al. Combination of baicalein and miR-106a-5p mimics significantly alleviates IL-1ß-induced inflammatory injury in CHON-001 cells. Exp Ther Med 21:345 (2021). PubMed: 33732318

View all Publications for this product

Customer reviews and Q&As

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1-10 of 23 Abreviews or Q&A

Application

Western blot

Sample

Human Cell lysate - whole cell (MCF7 cells lysate)

Gel Running Conditions

Reduced Denaturing (4–20% precast polyacrylamide gel)

Loading amount

20 µg

Specification

MCF7 cells lysate

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Apr 28 2022

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (Panc1 Tumor)

Antigen retrieval step

Heat mediated

Permeabilization

Specification

Panc1 Tumor

Blocking step

Bloxall as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 20°C

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Dec 30 2021

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Mouse Tissue sections (embryonic mouse spinal cord)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: 1x DAKO antigen retrieval buffer

Permeabilization

Yes - 0.3% Triton X-100 in PBS for 5 minutes

Specification

embryonic mouse spinal cord

Blocking step

Serum as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 21°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Dec 03 2021

Application

Immunohistochemistry (PFA perfusion fixed frozen sections)

Sample

Mouse Tissue sections (Intestine)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: 100X Citrate Buffer pH 6.0

Permeabilization

Yes - 0.05% tween 20

Specification

Intestine

Blocking step

Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Mar 18 2020

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Mouse Tissue sections (Spleen)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: 100X Citrate Buffer pH 6.0

Permeabilization

Yes - 0.05% tween 20

Specification

Spleen

Blocking step

Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 14 2020

Application

Western blot

Sample

Lizard Tissue lysate - whole (brain)

Gel Running Conditions

Reduced Denaturing (4-20%, mini-PROTEAN TGX Gel)

Loading amount

60 µg

Specification

brain

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Oliver Griffith

Verified customer

Submitted Oct 03 2019

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (Liver)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: 100X Citrate Buffer pH 6.0

Permeabilization

Yes - 0.05% tween 20

Specification

Liver

Blocking step

sea block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. David Ivancic

Verified customer

Submitted Jun 16 2019

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Mouse Tissue sections (Balb/C liver section)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Citrate pH 6

Permeabilization

Specification

Balb/C liver section

Blocking step

Innovex Background Buster as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 20°C

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Dec 26 2018

Application

Western blot

Sample

Human Cell lysate - other (Lysate of cytosol from MDA468)

Gel Running Conditions

Reduced Denaturing (8% TG gel)

Loading amount

50000 cells

Specification

Lysate of cytosol from MDA468

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 02 2017

Question

WB with human cancer cell lines sees consistent band at 110-120 kDa predicted MW is 270 for full length unprocessed precursor sent database link and explanation

Abcam community

Verified customer

Asked on Nov 03 2011

Answer

Thank you for contacting us. The database entry for the human NOtch1 protein is as follows: http://www.uniprot.org/uniprot/P46531 There you will find more information about the protein structure, sequences and additional references. In regards as to why you see band at 110-120 kDa: The predicted MW is 270 kDa for the full length unprocessed precursor. If the protein has splice variants or can be cleaved, than shorter versions of the protein can be detected - if they contain the immunogen sequence (cytoplasmic portion). According to the database entry, the protein can get cleaved into Notch 1 extracellular truncation and Notch 1 intracellular domain. The antibody would be able to recognize then the intracellular domain. However, under which condition the cleavage happens, I am not certain. I would suggest to consult the literature for this detailed information. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Abcam Scientific Support

Answered on Nov 03 2011

1-10 of 23 Abreviews or Q&A

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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Specificity

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Conjugation kits

Isotype control

KO cell lines

KO cell lysates

Recombinant Protein

Applications

The Abpromise guarantee

Target

Function

Tissue specificity

Involvement in disease

Sequence similarities

Post-translationalmodifications

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

References (140)

Customer reviews and Q&As

Western blot abreview for Anti-Notch1 antibody [EP1238Y]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Notch1 antibody [EP1238Y]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Notch1 antibody [EP1238Y]

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Notch1 antibody [EP1238Y]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Notch1 antibody [EP1238Y]

Western blot abreview for Anti-Notch1 antibody [EP1238Y]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Notch1 antibody [EP1238Y]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Notch1 antibody [EP1238Y]

Western blot abreview for Anti-Notch1 antibody [EP1238Y]

Post-translational
modifications