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    products/primary-antibodies/nox2gp91phox-antibody-epr6991-ab129068.pdf

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RecombinantRabMAb

Recombinant Anti-NOX2/gp91phox antibody [EPR6991] (ab129068)

  • Datasheet
  • SDS
Reviews (4) Submit a question References (94)

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Western blot - Anti-NOX2/gp91phox antibody [EPR6991] (ab129068)
  • Western blot - Anti-NOX2/gp91phox antibody [EPR6991] (ab129068)
  • Western blot - Anti-NOX2/gp91phox antibody [EPR6991] (ab129068)
  • OI-RD Scanning - Anti-NOX2/gp91phox antibody [EPR6991] (ab129068)
  • Anti-NOX2/gp91phox antibody [EPR6991] (ab129068)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6991] to NOX2/gp91phox
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Overview

  • Product name

    Anti-NOX2/gp91phox antibody [EPR6991]
    See all NOX2/gp91phox primary antibodies
  • Description

    Rabbit monoclonal [EPR6991] to NOX2/gp91phox
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human NOX2/gp91phox aa 150-250. The exact sequence is proprietary.

  • Positive control

    • WB: MCF-7, HepG2 and Caco-2 cell lysates, mouse and rat brain and spleen tissue lysates.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 2.70 x 10 -10 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR6991
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Adaptive Immunity
    • T Cells
    • Cytotoxic Cells
    • Cancer
    • Cancer Metabolism
    • Cellular metabolic process

Associated products

  • Alternative Versions

    • Anti-NOX2/gp91phox antibody [EPR6991] - BSA and Azide free (ab238964)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • MCF7 membrane extract lysate (ab29539)
  • Recombinant Protein

    • Recombinant Human NOX2/gp91phox protein (ab114183)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)
    • Mouse spleen tissue lysate - total protein (ab29293)
    • Mouse brain tissue lysate - total protein (ab4022)
    • Mouse brain tissue lysate - total protein (0 days) (ab7188)
    • Mouse brain tissue lysate - total protein (14 days) (ab7189)
    • Mouse brain tissue lysate - total protein (7 days) (ab7190)
    • Mouse spleen tissue lysate - total protein (14 days) (ab7274)
    • Mouse spleen tissue lysate - total protein (ab7937)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab129068 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (4)
1/5000. Detects a band of approximately 60 kDa (predicted molecular weight: 65 kDa).

For unpurified use at 1/1000 - 1/10000.

Notes
WB
1/5000. Detects a band of approximately 60 kDa (predicted molecular weight: 65 kDa).

For unpurified use at 1/1000 - 1/10000.

Application notes
Is unsuitable for Flow Cyt,ICC/IF or IHC-P.

Target

  • Function

    Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. Also functions as a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes. It participates in the regulation of cellular pH and is blocked by zinc.
  • Involvement in disease

    Defects in CYBB are a cause of chronic granulomatous disease X-linked (XCGD) [MIM:306400]. Chronic granulomatous disease is a genetically heterogeneous disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections.
  • Sequence similarities

    Contains 1 FAD-binding FR-type domain.
    Contains 1 ferric oxidoreductase domain.
  • Post-translational
    modifications

    Glycosylated.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P04839 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 1536 Human
    • Entrez Gene: 13058 Mouse
    • Entrez Gene: 66021 Rat
    • Omim: 300481 Human
    • SwissProt: P04839 Human
    • SwissProt: Q61093 Mouse
    • Unigene: 292356 Human
    • Unigene: 200362 Mouse
    • Alternative names

      • AMCBX2 antibody
      • CGD antibody
      • CGD91-phox antibody
      • CY24B_HUMAN antibody
      • CYBB antibody
      • Cytochrome b 245, beta polypeptide antibody
      • Cytochrome b(558) beta chain antibody
      • Cytochrome b(558) subunit beta antibody
      • Cytochrome b-245 heavy chain antibody
      • Cytochrome b558 subunit beta antibody
      • GP91 PHOX antibody
      • gp91-1 antibody
      • gp91-phox antibody
      • GP91PHOX antibody
      • Heme-binding membrane glycoprotein gp91phox antibody
      • NADPH oxidase 2 antibody
      • Neutrophil cytochrome b 91 kDa polypeptide antibody
      • NOX2 antibody
      • p22 phagocyte B-cytochrome antibody
      • P91 PHOX antibody
      • p91-PHOX antibody
      • Superoxide-generating NADPH oxidase heavy chain subunit antibody
      see all

    Images

    • Western blot - Anti-NOX2/gp91phox antibody [EPR6991] (ab129068)
      Western blot - Anti-NOX2/gp91phox antibody [EPR6991] (ab129068)
      All lanes : Anti-NOX2/gp91phox antibody [EPR6991] (ab129068) at 1/5000 dilution (purified)

      Lane 1 : Mouse brain tissue lysate
      Lane 2 : Mouse spleen tissue lysate
      Lane 3 : Rat brain tissue lysate
      Lane 4 : Rat spleen tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 65 kDa
      Observed band size: 60 kDa why is the actual band size different from the predicted?



      Blocking and diluting buffer: 5% NFDM/TBST.

    • Western blot - Anti-NOX2/gp91phox antibody [EPR6991] (ab129068)
      Western blot - Anti-NOX2/gp91phox antibody [EPR6991] (ab129068)
      All lanes : Anti-NOX2/gp91phox antibody [EPR6991] (ab129068) at 1/5000 dilution (purified)

      Lane 1 : MCF-7 (Human breast adenocarcinoma cell line) cell lysate
      Lane 2 : HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate
      Lane 3 : Caco-2 (Human colorectal adenocarcinoma cell line) cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 65 kDa
      Observed band size: 60 kDa why is the actual band size different from the predicted?



      Blocking and diluting buffer: 5% NFDM/TBST.

    • Western blot - Anti-NOX2/gp91phox antibody [EPR6991] (ab129068)
      Western blot - Anti-NOX2/gp91phox antibody [EPR6991] (ab129068)
      All lanes : Anti-NOX2/gp91phox antibody [EPR6991] (ab129068) at 1/1000 dilution (unpurified)

      Lane 1 : MCF-7 cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : Caco-2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size: 65 kDa
      Observed band size: 60 kDa why is the actual band size different from the predicted?

    • OI-RD Scanning - Anti-NOX2/gp91phox antibody [EPR6991] (ab129068)
      OI-RD Scanning - Anti-NOX2/gp91phox antibody [EPR6991] (ab129068)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Anti-NOX2/gp91phox antibody [EPR6991] (ab129068)
      Anti-NOX2/gp91phox antibody [EPR6991] (ab129068)

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (94)

    Publishing research using ab129068? Please let us know so that we can cite the reference in this datasheet.

    ab129068 has been referenced in 94 publications.

    • Mello A  et al. Soluble Epoxide Hydrolase Hepatic Deficiency Ameliorates Alcohol-Associated Liver Disease. Cell Mol Gastroenterol Hepatol 11:815-830 (2021). PubMed: 33068774
    • Tong Y  et al. Lotus leaf extract inhibits ER- breast cancer cell migration and metastasis. Nutr Metab (Lond) 18:20 (2021). PubMed: 33602253
    • Cheng W  et al. Paraventricular Nucleus P2X7 Receptors Aggravate Acute Myocardial Infarction Injury via ROS-Induced Vasopressin-V1b Activation in Rats. Neurosci Bull 37:641-656 (2021). PubMed: 33620697
    • Cao JY  et al. Autophagosome protects proximal tubular cells from aldosterone-induced senescence through improving oxidative stress. Ren Fail 43:556-565 (2021). PubMed: 33757397
    • Hatinguais R  et al. Mitochondrial Reactive Oxygen Species Regulate Immune Responses of Macrophages to Aspergillus fumigatus. Front Immunol 12:641495 (2021). PubMed: 33841423
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-4 of 4 Abreviews or Q&A

    Western blot abreview for Anti-NOX2/gp91phox antibody [EPR6991]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Pig Tissue lysate - whole (Kidney)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    35 µg
    Specification
    Kidney
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Nov 28 2017

    Western blot abreview for Anti-NOX2/gp91phox antibody [EPR6991]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (heart)
    Gel Running Conditions
    Reduced Denaturing (9%)
    Loading amount
    60 µg
    Treatment
    TNF-a 30min
    Specification
    heart
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Baptiste Jude

    Verified customer

    Submitted Jul 06 2016

    Western blot abreview for Anti-NOX2/gp91phox antibody [EPR6991]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    10 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Human Cell lysate - whole cell (Dermal fibroblast)
    Specification
    Dermal fibroblast
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Karina Ricart

    Verified customer

    Submitted Jul 31 2014

    Western blot abreview for Anti-NOX2/gp91phox antibody [EPR6991]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (EA. hy926)
    Loading amount
    10 µg
    Specification
    EA. hy926
    Gel Running Conditions
    Non-reduced Denaturing (10%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jan 02 2013

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