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RecombinantRabMAb

Recombinant Anti-NOXA2/p67phox antibody [EPR5064] (ab109366)

  • Datasheet
  • SDS
Reviews (3)Q&A (1)References (13)

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Western blot - Anti-NOXA2/p67phox antibody [EPR5064] (ab109366)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOXA2/p67phox antibody [EPR5064] (ab109366)
  • Anti-NOXA2/p67phox antibody [EPR5064] (ab109366)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5064] to NOXA2/p67phox
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free

You may also be interested in

Protein
Product image
Recombinant Human NOXA2/p67phox protein (ab117065)
Primary
Product image
Anti-NOX2/gp91phox antibody [EPR6991] (ab129068)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)

View more associated products

Overview

  • Product name

    Anti-NOXA2/p67phox antibody [EPR5064]
    See all NOXA2/p67phox primary antibodies
  • Description

    Rabbit monoclonal [EPR5064] to NOXA2/p67phox
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human spleen tissue. THP-1 cell lysate. HL-60 cells treated with dexomethasome, dimethyl sulfoxide, and TPA
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR5064
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Adaptive Immunity
    • T Cells
    • Cytotoxic Cells
    • Cancer
    • Cancer Metabolism
    • Cellular metabolic process
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Alternative Versions

    • Alexa Fluor® 488 Anti-NOXA2/p67phox antibody [EPR5064] (ab206256)
    • Alexa Fluor® 647 Anti-NOXA2/p67phox antibody [EPR5064] (ab206257)
    • Anti-NOXA2/p67phox antibody [EPR5064] - BSA and Azide free (ab239969)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human NOXA2/p67phox protein (ab117065)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab109366 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (3)
1/1000 - 1/10000. Predicted molecular weight: 60 kDa.
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 60 kDa.
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Application notes
Is unsuitable for Flow Cyt.

Target

  • Function

    NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).
  • Involvement in disease

    Defects in NCF2 are a cause of chronic granulomatous disease autosomal recessive cytochrome-b-positive type 2 (CGD2) [MIM:233710]. Chronic granulomatous disease is a genetically heterogeneous disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections.
  • Sequence similarities

    Belongs to the NCF2/NOXA1 family.
    Contains 1 OPR domain.
    Contains 2 SH3 domains.
    Contains 3 TPR repeats.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession P19878 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 4688 Human
    • Entrez Gene: 17970 Mouse
    • Entrez Gene: 364018 Rat
    • Omim: 608515 Human
    • SwissProt: P19878 Human
    • SwissProt: O70145 Mouse
    • SwissProt: Q3U5S4 Mouse
    • SwissProt: A7E3N2 Rat
    • Unigene: 587558 Human
    • Unigene: 270307 Mouse
    • Unigene: 162331 Rat
    see all
  • Alternative names

    • 67 kDa neutrophil oxidase factor antibody
    • Chronic granulomatous disease autosomal 2 antibody
    • FLJ93058 antibody
    • NADPH oxidase activator 2 antibody
    • NCF-2 antibody
    • Ncf2 antibody
    • NCF2_HUMAN antibody
    • Neutrophil cytosol factor 2 antibody
    • Neutrophil cytosolic factor 2 (65kD, chronic granulomatous disease, autosomal 2) antibody
    • Neutrophil NADPH oxidase factor 2 antibody
    • NOXA2 antibody
    • P67 PHOX antibody
    • p67-phox antibody
    • p67phox antibody
    see all

Images

  • Western blot - Anti-NOXA2/p67phox antibody [EPR5064] (ab109366)
    Western blot - Anti-NOXA2/p67phox antibody [EPR5064] (ab109366)
    All lanes : Anti-NOXA2/p67phox antibody [EPR5064] (ab109366) at 1/1000 dilution

    Lane 1 : HL-60 cells treated with dexomethasome, dimethyl sulfoxide, and TPA
    Lane 2 : THP-1 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled Goat anti-Rabbit at 1/2000 dilution

    Predicted band size: 60 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOXA2/p67phox antibody [EPR5064] (ab109366)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOXA2/p67phox antibody [EPR5064] (ab109366)

    Immunohistochemical analysis of NOXA2/p67phox in paraffin-embedded Human spleen tissue using ab109366 at 1/500 dilution

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-NOXA2/p67phox antibody [EPR5064] (ab109366)
    Anti-NOXA2/p67phox antibody [EPR5064] (ab109366)

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (13)

Publishing research using ab109366? Please let us know so that we can cite the reference in this datasheet.

ab109366 has been referenced in 13 publications.

  • Yang X  et al. Enhanced cardiomyocyte reactive oxygen species signaling promotes ibrutinib-induced atrial fibrillation. Redox Biol 30:101432 (2020). PubMed: 31986467
  • Duan M  et al. Indigo Fruits Ingredient, Aucubin, Protects against LPS-Induced Cardiac Dysfunction in Mice. J Pharmacol Exp Ther 371:348-359 (2019). PubMed: 31467086
  • Chen HH  et al. Antihypertensive Potential of Coenzyme Q10 via Free Radical Scavenging and Enhanced Akt-nNOS Signaling in the Nucleus Tractus Solitarii in Rats. Mol Nutr Food Res 63:e1801042 (2019). PubMed: 30668894
  • Zhang Y  et al. YiQiFuMai Powder Injection Attenuates Coronary Artery Ligation-Induced Heart Failure Through Improving Mitochondrial Function via Regulating ROS Generation and CaMKII Signaling Pathways. Front Pharmacol 10:381 (2019). PubMed: 31031629
  • Huang C  et al. Interaction Mechanisms Between the NOX4/ROS and RhoA/ROCK1 Signaling Pathways as New Anti- fibrosis Targets of Ursolic Acid in Hepatic Stellate Cells. Front Pharmacol 10:431 (2019). PubMed: 31130857
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
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1-4 of 4 Abreviews or Q&A

Western blot abreview for Anti-NOXA2/p67phox antibody [EPR5064]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (THP-1 cells)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
10 µg
Specification
THP-1 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jun 12 2017

Western blot abreview for Anti-NOXA2/p67phox antibody [EPR5064]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rat Tissue lysate - whole (Skeletal muscle)
Gel Running Conditions
Reduced Denaturing (8%)
Loading amount
20 µg
Specification
Skeletal muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 01 2016

Western blot abreview for Anti-NOXA2/p67phox antibody [EPR5064]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Skeletal muscle)
Gel Running Conditions
Reduced Denaturing (8%)
Loading amount
20 µg
Specification
Skeletal muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 01 2016

Question

Sehr geehrte Damen und Herren, wir möchten in unserer Arbeitsgruppe beginnen, mit der NADPH-oxidase in Makrophagen (Rattenmakrophagen NR8383, ev. auch humane Monozyten/Makrophagen U937) zu arbeiten und sind auf der Suche nach Antikörpern gegen ihre Bestandteile (p47PHOX, p40PHOX, p67PHOX, P22PHOX, gp91PHOX, Rac1/2, Rho-GDI, …), sowie (falls möglich) die Einzelkomponenten selber (für die Arbeit im zellfreien System). Können Sie uns weiterhelfen? Über ein Angebot würden wir uns freuen.

Read More

Abcam community

Verified customer

Asked on Dec 09 2011

Answer

Vielen Dank für Ihre Anfrage. Gegen die genannten NADPH-Oxidase-Komponenten haben wir folgende Antikörper bzw. Proteine in unserem Katalog: Click here (or use the following: https://www.abcam.com/NOXA2p67phox-antibody-EPR5064-ab109366.html). Click here (or use the following: https://www.abcam.com/NOX2gp91phox-antibody-ab80508.html). Click here (or use the following: https://www.abcam.com/Rac1-antibody-23A8-ab33186.html). Click here (or use the following: https://www.abcam.com/RAC2-antibody-ab2244.html). Click here (or use the following: https://www.abcam.com/RhoGDI-antibody-OTI1F2-ab118159.html). Click here (or use the following: https://www.abcam.com/Cytochrome-b245-Light-Chainp22-phox-antibody-ab75941.html). Click here (or use the following: https://www.abcam.com/p40-phox-antibody-ab115855.html). Click here (or use the following: https://www.abcam.com/p40-phox-phospho-T154-antibody-ab59355.html). Click here (or use the following: https://www.abcam.com/NCF1p47-phox-phospho-S328-antibody-ab111855.html). Click here (or use the following: https://www.abcam.com/NCF1p47-phox-antibody-ab795.html). Click here (or use the following: https://www.abcam.com/Human-NCF4-full-length-protein-ab88031.html). Click here (or use the following: https://www.abcam.com/Recombinant-Human-NCF1p47-phox-protein-ab98054.html). Click here (or use the following: https://www.abcam.com/Recombinant-Human-NOXA2p67phox-protein-ab117065.html). Click here (or use the following: https://www.abcam.com/Recombinant-Human-NOX2gp91phox-protein-ab114183.html). Click here (or use the following: https://www.abcam.com/Recombinant-Human-Rac1-protein-ab84657.html). Click here (or use the following: https://www.abcam.com/Human-Rac1-full-length-protein-ab40471.html). Click here (or use the following: https://www.abcam.com/Recombinant-Human-Rac1-protein-ab89246.html). Click here (or use the following: https://www.abcam.com/Recombinant-Human-Rac1-protein-ab82670.html). Click here (or use the following: https://www.abcam.com/Recombinant-Human-Rac1-protein-ab51014.html). Click here (or use the following: https://www.abcam.com/Recombinant-Human-RAC2-protein-ab84655.html). Click here (or use the following: https://www.abcam.com/Recombinant-Human-RAC2-protein-ab78684.html). Click here (or use the following: https://www.abcam.com/Recombinant-Human-RAC2-protein-ab87665.html). Vielleicht interessiert Sie in diesem Zusammenhang auch einer unserer Assaykits (NADP/NADPH): Click here (or use the following: https://www.abcam.com/NADPNADPH-Assay-Kit-ab65349.html). Wir geben alle getesteten Spezies und Anwendungen auf unseren Produktdatenblättern an und aktualisieren diese sobald wir neue Informationen erhalten. Falls sich herausstellt, dass der Antikörper nicht so funktioniert, wie auf dem Datenblatt beschrieben und Sie sich innerhalb von sechs Monaten mit Ihrem Problem an uns wenden, werden wir Ihnen gerne Vorschläge zur Optimierung Ihres Protokolls oder gegebenenfalls einen Ersatz oder eine Gutschrift schicken. Falls der Antikörper in einer bisher nicht getesteten Anwendung oder Spezies verwendet wird (z.B. für ab111855 oder ab115855), qualifizieren Sie sich für unser Testprogramm: Bei diesem Angebot bekommen Sie einen 100%igen Rabatt für eine zukünftige Bestellung, wenn Sie uns ein Abreview mit dem Testresultat zusenden. Die Bedingungen zu unserem 100% Abreview Rabatt können Sie unter dem folgenden Link nachlesen: https://www.abcam.com/collaborationdiscount. Falls Sie daran interessiert sind oder weitere Fragen haben, kontaktieren Sie bitte unseren wissenschaftlichen Dienst. Die oben genannten Antikörper sind nur eine Auswahl und wir haben noch weitere gegen die entsprechenden Zielproteine erhältlich, die für unterschiedliche Anwendungen getestet und garantiert sind. Bei einer detaillierteren Suche bzw. Beratung bin ich Ihnen gerne weiter behilflich. Gerne senden wir Ihnen auch gerne ein Angebot zu, sobald Sie eine konkrete Auswahl getroffen haben. Ich hoffe, diese Informationen helfen Ihnen weiter. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

Read More

Abcam Scientific Support

Answered on Dec 09 2011

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