Recombinant Anti-NOXA2/p67phox antibody [EPR5064] - BSA and Azide free (ab239969)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOXA2/p67phox antibody [EPR5064] - BSA and Azide free (ab239969)](https://www.abcam.com/ps/products/239/ab239969/Images/ab239969-1-NOXA2p67phoxPrimaryantibodiesab1093663.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOXA2/p67phox antibody [EPR5064] - BSA and Azide free (ab239969)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5064] to NOXA2/p67phox - BSA and Azide free
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-NOXA2/p67phox antibody [EPR5064] - BSA and Azide free
See all NOXA2/p67phox primary antibodies -
Description
Rabbit monoclonal [EPR5064] to NOXA2/p67phox - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab239969 is the carrier-free version of ab109366.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5064 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Immunohistochemistry kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab239969 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 60 kDa.
|
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| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| Notes |
|---|
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WB
Use at an assay dependent concentration. Predicted molecular weight: 60 kDa. |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production). -
Involvement in disease
Defects in NCF2 are a cause of chronic granulomatous disease autosomal recessive cytochrome-b-positive type 2 (CGD2) [MIM:233710]. Chronic granulomatous disease is a genetically heterogeneous disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections. -
Sequence similarities
Belongs to the NCF2/NOXA1 family.
Contains 1 OPR domain.
Contains 2 SH3 domains.
Contains 3 TPR repeats. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 4688 Human
- Entrez Gene: 17970 Mouse
- Entrez Gene: 364018 Rat
- Omim: 608515 Human
- SwissProt: P19878 Human
- SwissProt: O70145 Mouse
- SwissProt: Q3U5S4 Mouse
- SwissProt: A7E3N2 Rat
see all -
Alternative names
- 67 kDa neutrophil oxidase factor antibody
- Chronic granulomatous disease autosomal 2 antibody
- FLJ93058 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOXA2/p67phox antibody [EPR5064] - BSA and Azide free (ab239969)
Immunohistochemical analysis of NOXA2/p67phox in paraffin-embedded Human spleen tissue using ab109366 at 1/500 dilution
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109366).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-NOXA2/p67phox antibody [EPR5064] - BSA and Azide free (ab239969)](https://www.abcam.com/ps/products/239/ab239969/Images/ab239969-2-benefits-of-recombinant-antibodies.png)
Anti-NOXA2/p67phox antibody [EPR5064] - BSA and Azide free (ab239969)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab239969 has not yet been referenced specifically in any publications.
