Recombinant Anti-NPR-A + NPR-B antibody [EPR5719(2)] (ab139188)
![Western blot - Anti-NPR-A + NPR-B antibody [EPR5719(2)] (ab139188)](https://www.abcam.com/ps/products/139/ab139188/Images/ab139188-467541-anti-npr-a-npr-b-antibody-epr5719-2-westernblot-human-mouse-rat.jpg "Western blot - Anti-NPR-A + NPR-B antibody [EPR5719(2)] (ab139188)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5719(2)] to NPR-A + NPR-B
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-NPR-A + NPR-B antibody [EPR5719(2)] -
Description
Rabbit monoclonal [EPR5719(2)] to NPR-A + NPR-B -
Host species
Rabbit -
Specificity
The immunogen for this product shares 95% homology with NPR-A -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
(Peptide available asab150296) -
Positive control
- WB: Human heart and kidney lysate. Mouse brain and heart lysate. Rat brain and heart lysate. GST-tagged recombinant human Natriuretic Peptide Receptor A and B protein.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5719(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab139188 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 117 kDa.
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| Notes |
|---|
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WB
1/1000 - 1/10000. Predicted molecular weight: 117 kDa. |
Target
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Cellular localization
Natriuretic Peptide Receptor A/GC-A: Membrane. Natriuretic Peptide Receptor B: Membrane. -
Database links
- Entrez Gene: 4881 Human
- Entrez Gene: 4882 Human
- Entrez Gene: 18160 Mouse
- Entrez Gene: 230103 Mouse
- Entrez Gene: 116564 Rat
- Entrez Gene: 24603 Rat
- Omim: 108960 Human
- Omim: 108961 Human
see all
Images
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Western blot - Anti-NPR-A + NPR-B antibody [EPR5719(2)] (ab139188)All lanes : Anti-NPR-A + NPR-B antibody [EPR5719(2)] (ab139188) at 1/10000 dilution (Purified)
Lane 1 : Human heart lysate
Lane 2 : Human kidney lysate
Lane 3 : Mouse brain lysate
Lane 4 : Mouse heart lysate
Lane 5 : Rat brain lysate
Lane 6 : Rat heart lysate
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 117 kDa -
![Western blot - Anti-NPR-A + NPR-B antibody [EPR5719(2)] (ab139188)](https://www.abcam.com/ps/products/139/ab139188/Images/ab139188-450409-anti-natriuretic-peptide-receptor-a-b-antibody-epr5719-2-western-blot-recombinant-human.jpg)
Western blot - Anti-NPR-A + NPR-B antibody [EPR5719(2)] (ab139188)All lanes : Anti-NPR-A + NPR-B antibody [EPR5719(2)] (ab139188) at 1/1000 dilution
Lane 1 : GST-tagged recombinant human Natriuretic Peptide Receptor A protein (aa 495 to 1061)
Lane 2 : GST-tagged recombinant human Natriuretic Peptide Receptor B protein (aa 479 to 1047)
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 117 kDa
Observed band size: 88 kDa why is the actual band size different from the predicted?
Exposure time: 1 secondBlocking buffer and concentration: 5% NFDM/TBST
Loading Control: Anti-GST antibody, ab111947
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![Anti-NPR-A + NPR-B antibody [EPR5719(2)] (ab139188)](https://www.abcam.com/ps/products/139/ab139188/Images/ab139188-3-benefits-of-recombinant-antibodies.png)
Anti-NPR-A + NPR-B antibody [EPR5719(2)] (ab139188)
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab139188 has been referenced in 3 publications.
- Hirota K et al. C-type Natriuretic Peptide-induced PKA Activation Promotes Endochondral Bone Formation in Hypertrophic Chondrocytes. Endocrinology 163:N/A (2022). PubMed: 35041746
- Wang Q et al. Identification of RyR2-PBmice and the effects of transposon insertional mutagenesis of the RyR2 gene on cardiac function in mice. PeerJ 7:e6942 (2019). PubMed: 31143551
- Ding DZ et al. C-type natriuretic peptide prevents angiotensin II-induced atrial connexin 40 and 43 dysregulation by activating AMP-activated kinase signaling. Mol Med Rep 20:5091-5099 (2019). PubMed: 31638216