Recombinant Anti-NQO2 antibody [EPR13345] - BSA and Azide free (ab250309)
![Western blot - Anti-NQO2 antibody [EPR13345] - BSA and Azide free (ab250309)](https://www.abcam.com/ps/products/250/ab250309/Images/ab250309-405765-anti-nqo2-antibody-epr13345-bsa-and-azide-free-western-blot.jpg "Western blot - Anti-NQO2 antibody [EPR13345] - BSA and Azide free (ab250309)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13345] to NQO2 - BSA and Azide free
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Mouse, Rat
Related conjugates and formulations
Overview
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Product name
Anti-NQO2 antibody [EPR13345] - BSA and Azide free
See all NQO2 primary antibodies -
Description
Rabbit monoclonal [EPR13345] to NQO2 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details -
Species reactivity
Reacts with: Mouse, Rat
Predicted to work with: Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab250309 is the carrier-free version of ab181049.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR13345 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab250309 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
Use at an assay dependent concentration.
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| WB |
Use at an assay dependent concentration. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
|
| Notes |
|---|
|
Flow Cyt (Intra)
Use at an assay dependent concentration. |
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WB
Use at an assay dependent concentration. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa). |
Target
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Function
The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinones involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. -
Sequence similarities
Belongs to the NAD(P)H dehydrogenase (quinone) family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 4835 Human
- Entrez Gene: 18105 Mouse
- Entrez Gene: 291084 Rat
- Omim: 160998 Human
- SwissProt: P16083 Human
- SwissProt: Q9JI75 Mouse
- SwissProt: Q6AY80 Rat
- Unigene: 533050 Human
see all -
Alternative names
- DHQV antibody
- DIA6 antibody
- EC 1.10.99.2 antibody
see all
Images
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Western blot - Anti-NQO2 antibody [EPR13345] - BSA and Azide free (ab250309)All lanes : Anti-NQO2 antibody [EPR13345] (ab181049) at 1/1000 dilution
Lane 1 : Mouse kidney tissue lysate
Lane 2 : Rat heart tissue lysate
Lane 3 : Rat kidney tissue lysate
Lane 4 : Rat spleen tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 26 kDaThis data was developed using ab181049, the same antibody clone in a different buffer formulation.
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![Flow Cytometry (Intracellular) - Anti-NQO2 antibody [EPR13345] - BSA and Azide free (ab250309)](https://www.abcam.com/ps/products/250/ab250309/Images/ab250309-2-anti-nqo2-antibody-epr13345-bsa-and-azide-free-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-NQO2 antibody [EPR13345] - BSA and Azide free (ab250309)This data was developed using ab181049, the same antibody clone in a different buffer formulation.
Intracellular flow cytometric analysis of C6 cells labeling NQO2 with ab181049 at 1/140 dilution (red). Goat anti rabbit IgG (FITC) used as the secondary at 1/150 dilution. Isotype control Rabbit monoclonal IgG (green). Cells were fixed in 2% paraformaldehyde.
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![Western blot - Anti-NQO2 antibody [EPR13345] - BSA and Azide free (ab250309)](https://www.abcam.com/ps/products/250/ab250309/Images/ab250309-398560-anti-nqo2-antibody-epr13345-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-NQO2 antibody [EPR13345] - BSA and Azide free (ab250309)All lanes : Anti-NQO2 antibody [EPR13345] (ab181049) at 1/5000 dilution
Lane 1 : L6 cell lysate
Lane 2 : C2C12 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 26 kDaThis data was developed using ab181049, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Anti-NQO2 antibody [EPR13345] - BSA and Azide free (ab250309)](https://www.abcam.com/ps/products/250/ab250309/Images/ab250309-4-benefits-of-recombinant-antibodies.png)
Anti-NQO2 antibody [EPR13345] - BSA and Azide free (ab250309)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab250309 has not yet been referenced specifically in any publications.
