Name: Anti-NR2F2 antibody [EPR18443]
Brand: RabMAb
SKU: ab211777
Rating: 5 (2 reviews)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR2F2 antibody [EPR18443] (ab211777)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR18443] to NR2F2
Suitable for: WB, IHC-P, ICC/IF, IP
Reacts with: Mouse, Rat, Human

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Carrier Free

Product name

Anti-NR2F2 antibody [EPR18443]
See all NR2F2 primary antibodies
Description

Rabbit monoclonal [EPR18443] to NR2F2
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-P, ICC/IF, IPmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HCT116, C6, PC-12, NIH/3T3, MCF7, K562, HEK-293 and HEK-293T whole cell lysates; human fetal kidney tissue lysate; rat brain and spleen tissue lysates. IHC-P: Human tonsil, testis, fetal spleen, breast cancer and colon cancer tissue; mouse liver tissue; rat lung tissue. ICC/IF: NIH/3T3 and HEK-293 cells. IP: MCF7 whole cel lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR18443
Isotype

IgG
Research areas

Alternative Versions
Compatible Secondaries
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
KO cell lines
- Human NR2F2 knockout HCT116 cell line (ab266888)
KO cell lysates
- Human NR2F2 knockout HCT116 cell lysate (ab257186)
Recombinant Protein
- Recombinant Human NR2F2 protein (ab159680)
Related Products
- Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab211777 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/1000. Predicted molecular weight: 46 kDa.
IHC-P		1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF		1/100.
IP		1/50.

Notes
WB 1/1000. Predicted molecular weight: 46 kDa.
IHC-P 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/100.
IP 1/50.

Function

Ligand-activated transcription factor. Activated by high concentrations of 9-cis-retinoic acid and all-trans-retinoic acid, but not by dexamethasone, cortisol or progesterone (in vitro). Regulation of the apolipoprotein A-I gene transcription. Binds to DNA site A.
Tissue specificity

Ubiquitous.
Involvement in disease

Congenital heart defects, multiple types, 4
Sequence similarities

Belongs to the nuclear hormone receptor family. NR2 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
Cellular localization

Nucleus.
Target information above from: UniProt accession P24468 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 7026 Human
- Entrez Gene: 11819 Mouse
- Entrez Gene: 113984 Rat
- Omim: 107773 Human
- SwissProt: P24468 Human
- SwissProt: P43135 Mouse
- SwissProt: O09018 Rat
- Unigene: 347991 Human
- Unigene: 158143 Mouse
- Unigene: 164334 Rat
- Unigene: 17815 Rat
see all
Alternative names
- Apolipoprotein A-I regulatory protein 1 antibody
- Apolipoprotein AI regulatory protein 1 antibody
- ARP-1 antibody
- ARP1 antibody
- COT2_HUMAN antibody
- COUP TF2 antibody
- COUP transcription factor 2 antibody
- COUP transcription factor II antibody
- COUP-TF II antibody
- COUP-TF2 antibody
- NR2F2 antibody
- Nuclear receptor subfamily 2 group F member 2 antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR2F2 antibody [EPR18443] (ab211777)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling NR2F2 with ab211777 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on mesenchymal cells of human tonsil tissue (PMID: 11026559) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-NR2F2 antibody [EPR18443] (ab211777)

Lane 1 : Anti-TRIM24 antibody [EPR22825-2] (ab256491) at 1/1000 dilution
Lane 2 : Anti-NR2F2 antibody [EPR18443] (ab211777) at 1/1000 dilution

Lane 1 : Wild-type HCT116 cell lysate
Lane 2 : NR2F2 CRISPR/Cas9 edited HCT116 cell lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 46 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?

Lanes 1- 2: Merged signal (red and green). Green - ab211777 observed at 45 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

ab211777 was shown to react with NR2F2 in wild-type HCT116 cells in western blot. The band observed in CRISPR/Cas9 edited cell line ab266888 (CRISPR/Cas9 edited cell lysate ab257186) lane below 45kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type HCT116 and NR2F2 CRISPR/Cas9 edited HCT116 cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab211777 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-NR2F2 antibody [EPR18443] (ab211777)

All lanes : Anti-NR2F2 antibody [EPR18443] (ab211777) at 1/1000 dilution

Lane 1 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Human fetal kidney tissue lysate
Lane 4 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
Lane 5 : K562 (human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
Lanes 1-2 & 4-5 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lane 3 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Developed using the ECL technique.

Predicted band size: 46 kDa
Observed band size: 46 kDa

Exposure time: 3 minutes

Blocking and dilution buffer: 5% NFDM /TBST
Western blot - Anti-NR2F2 antibody [EPR18443] (ab211777)

All lanes : Anti-NR2F2 antibody [EPR18443] (ab211777) at 1/1000 dilution

Lane 1 : C6 (rat glial tumor cell line) whole cell lysate
Lane 2 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 3 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 4 : Rat brain tissue lysate
Lane 5 : Rat spleen tissue lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 46 kDa
Observed band size: 46 kDa

Exposure time: 3 minutes

Blocking and dilution buffer: 5% NFDM /TBST
Western blot - Anti-NR2F2 antibody [EPR18443] (ab211777)

Anti-NR2F2 antibody [EPR18443] (ab211777) at 1/1000 dilution + Human NR2F1 full length recombinant protein (negative control) at 10 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 46 kDa

Exposure time: 3 minutes

Blocking and dilution buffer: 5% NFDM/TBST

Human NR2F1 full length recombinant protein containing aa1-423 with a His-tag was made in house.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR2F2 antibody [EPR18443] (ab211777)

Immunohistochemical analysis of paraffin-embedded human testis tissue labeling NR2F2 with ab211777 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on mesenchymal cells of human testis tissue (PMID: 11026559) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR2F2 antibody [EPR18443] (ab211777)

Immunohistochemical analysis of paraffin-embedded human fetal spleen tissue labeling NR2F2 with ab211777 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on mesenchymal cells of human fetal spleen tissue is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR2F2 antibody [EPR18443] (ab211777)

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling NR2F2 with ab211777 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on tumor cells of human breast cancer tissue is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR2F2 antibody [EPR18443] (ab211777)

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue labeling NR2F2 with ab211777 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on tumor cells of human colon cancer tissue is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR2F2 antibody [EPR18443] (ab211777)

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling NR2F2 with ab211777 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on mesenchymal cells of mouse liver tissue is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR2F2 antibody [EPR18443] (ab211777)

Immunohistochemical analysis of paraffin-embedded rat lung tissue labeling NR2F2 with ab211777 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on mesenchymal cells of rat lung tissue is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-NR2F2 antibody [EPR18443] (ab211777)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (mouse embryo fibroblast cell line) cells labeling NR2F2 with ab211777 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on NIH/3T3 cell line.

The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) (ab150120) secondary antibody at 1/1000 dilution (red).

The negative controls are as follows:
-ve control 1: ab211777 at 1/100 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) preadsorbed (ab150120) secondary at 1/1000 dilution.
-ve control 2: Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary at 1/1000 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-NR2F2 antibody [EPR18443] (ab211777)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293 (human epithelial cell line from embryonic kidney) cells labeling NR2F2 with ab211777 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on HEK293 cell line.

The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution (red).

The negative controls are as follows:
-ve control 1: ab211777 at 1/100 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) secondary at 1/1000 dilution.
-ve control 2: Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary at 1/1000 dilution.
Immunoprecipitation - Anti-NR2F2 antibody [EPR18443] (ab211777)

NR2F2 was immunoprecipitated from 1 mg of MCF7 (human breast adenocarcinoma cell line) whole cell lysate with ab211777 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab211777 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Lane 1: MCF7 whole cell lysate 10 μg (Input).
Lane 2: ab211777 IP in MCF7 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab211777 in MCF7 whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
Anti-NR2F2 antibody [EPR18443] (ab211777)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

Publishing research using ab211777? Please let us know so that we can cite the reference in this datasheet.

ab211777 has been referenced in 4 publications.

Xu Q & Jiang S miR-194-5p serves a suppressive role in human keloid fibroblasts via targeting NR2F2. Mol Med Rep 23:N/A (2021). PubMed: 33200800
Zhao G et al. Regeneration of the pulmonary vascular endothelium after viral pneumonia requires COUP-TF2. Sci Adv 6:N/A (2020). PubMed: 33239293
Yang D et al. Ginsenoside Rh2 induces DNA damage and autophagy in vestibular schwannoma is dependent of LAMP2 transcriptional suppression. Biochem Biophys Res Commun 522:300-307 (2020). PubMed: 31771882
Lang Q et al. COUP-TFII promotes metastasis and epithelial-to-mesenchymal transition through upregulating Snail in human intrahepatic cholangiocarcinoma. Acta Biochim Biophys Sin (Shanghai) 52:1247-1256 (2020). PubMed: 33166992

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Application

IHC - Wholemount

Sample

Rat Embryo (E13.5 Rat Embryo)

Specification

E13.5 Rat Embryo

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Alain Chedotal

Verified customer

Submitted Jan 31 2022

Application

Immunohistochemistry (Frozen sections)

Sample

Human Tissue sections (Human embryo ovary)

Permeabilization

Yes - Triton

Specification

Human embryo ovary

Blocking step

(agent) for 1 hour(s) and 0 minute(s) · Concentration: 0.25% · Temperature: 21°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Alain Chedotal

Verified customer

Submitted Mar 03 2021

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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Isotype control

KO cell lines

KO cell lysates

Recombinant Protein

Related Products

Applications

The Abpromise guarantee

Target

Function

Tissue specificity

Involvement in disease

Sequence similarities

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

Certificate of Compliance

References (4)

Customer reviews and Q&As

IHC - Wholemount abreview for Anti-NR2F2 antibody [EPR18443]

Immunohistochemistry (Frozen sections) abreview for Anti-NR2F2 antibody [EPR18443]