Anti-NR2F2 antibody [H7147] (ab41859)
Key features and details
- Mouse monoclonal [H7147] to NR2F2
- Suitable for: IP, ELISA, IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG2a
Overview
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Product name
Anti-NR2F2 antibody [H7147]
See all NR2F2 primary antibodies -
Description
Mouse monoclonal [H7147] to NR2F2 -
Host species
Mouse -
Tested applications
Suitable for: IP, ELISA, IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow -
Immunogen
Recombinant fragment:
PGQGGPAS TPAQTAAGGQ GGPG
, corresponding to amino acids 43-64 of Human NR2F2 -
Positive control
- Human Convoluted tubule and Rat Glomerular
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General notes
This product was changed from ascites to tissue culture supernatant on 3rd April 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7
Preservative: 0.1% Sodium azide
Physiological saline. -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
H7147 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab41859 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at an assay dependent concentration.
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ELISA |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Detects a band of approximately 49 kDa (predicted molecular weight: 46 kDa).
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ICC/IF | (1) |
Use at an assay dependent concentration.
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Notes |
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IP
Use at an assay dependent concentration. |
ELISA
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Detects a band of approximately 49 kDa (predicted molecular weight: 46 kDa). |
ICC/IF
Use at an assay dependent concentration. |
Target
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Function
Ligand-activated transcription factor. Activated by high concentrations of 9-cis-retinoic acid and all-trans-retinoic acid, but not by dexamethasone, cortisol or progesterone (in vitro). Regulation of the apolipoprotein A-I gene transcription. Binds to DNA site A. -
Tissue specificity
Ubiquitous. -
Involvement in disease
Congenital heart defects, multiple types, 4 -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR2 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 281945 Cow
- Entrez Gene: 7026 Human
- Entrez Gene: 11819 Mouse
- Entrez Gene: 113984 Rat
- Omim: 107773 Human
- SwissProt: Q9TTR7 Cow
- SwissProt: P24468 Human
- SwissProt: P43135 Mouse
see all -
Alternative names
- Apolipoprotein A-I regulatory protein 1 antibody
- Apolipoprotein AI regulatory protein 1 antibody
- ARP-1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR2F2 antibody [H7147] (ab41859)
ab41859 staining human NR2F2 in convoluted tubule by Immunohistochemistry (FFPE-sections) 10ug/ml.
This image was generated using the ascites version of the product.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR2F2 antibody [H7147] (ab41859)
ab41859 staining rat NR2F2 in Glomerular by Immunohistochemistry (FFPE-sections) 10ug/ml.
This image was generated using the ascites version of the product.
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All lanes : Anti-NR2F2 antibody [H7147] (ab41859) at 1 µg/ml
Lane 1 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 46 kDa
Observed band size: 49 kDa why is the actual band size different from the predicted?
Additional bands at: 72 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutesThis image was generated using the ascites version of the product.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (23)
ab41859 has been referenced in 23 publications.
- Polvani S et al. Isoforms of the orphan nuclear receptor COUP‑TFII differentially modulate pancreatic cancer progression. Int J Oncol 60:N/A (2022). PubMed: 35348189
- Meçe O et al. Lipid droplet degradation by autophagy connects mitochondria metabolism to Prox1-driven expression of lymphatic genes and lymphangiogenesis. Nat Commun 13:2760 (2022). PubMed: 35589749
- Wei JR et al. Identification of visual cortex cell types and species differences using single-cell RNA sequencing. Nat Commun 13:6902 (2022). PubMed: 36371428
- O'Leary TP et al. Neuronal cell types, projections, and spatial organization of the central amygdala. iScience 25:105497 (2022). PubMed: 36425768
- Aguiari P et al. Persistent COUP-TFII expression underlies the myopathy and impaired muscle regeneration observed in resistance to thyroid hormone-alpha. Sci Rep 11:4601 (2021). PubMed: 33633251