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Anti-NR2F2 antibody [H7147] (ab41859)

Reviews (2)Q&A (2)References (23)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR2F2 antibody [H7147] (ab41859)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR2F2 antibody [H7147] (ab41859)
  • Western blot - Anti-NR2F2 antibody [H7147] (ab41859)

Key features and details

  • Mouse monoclonal [H7147] to NR2F2
  • Suitable for: IP, ELISA, IHC-P, WB, ICC/IF
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG2a

Overview

  • Product name

    Anti-NR2F2 antibody [H7147]
    See all NR2F2 primary antibodies

  • Description

    Mouse monoclonal [H7147] to NR2F2

  • Host species

    Mouse

  • Tested applications

    Suitable for: IP, ELISA, IHC-P, WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow

  • Immunogen

    Recombinant fragment:

    PGQGGPAS TPAQTAAGGQ GGPG

    , corresponding to amino acids 43-64 of Human NR2F2
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Human Convoluted tubule and Rat Glomerular

  • General notes

    This product was changed from ascites to tissue culture supernatant on 3rd April 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab41859 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 49 kDa (predicted molecular weight: 46 kDa).
ICC/IF (1)
Use at an assay dependent concentration.
Notes
IP
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 49 kDa (predicted molecular weight: 46 kDa).
ICC/IF
Use at an assay dependent concentration.

Target

  • Function

    Ligand-activated transcription factor. Activated by high concentrations of 9-cis-retinoic acid and all-trans-retinoic acid, but not by dexamethasone, cortisol or progesterone (in vitro). Regulation of the apolipoprotein A-I gene transcription. Binds to DNA site A.

  • Tissue specificity

    Ubiquitous.

  • Involvement in disease

    Congenital heart defects, multiple types, 4

  • Sequence similarities

    Belongs to the nuclear hormone receptor family. NR2 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Apolipoprotein A-I regulatory protein 1 antibody
    • Apolipoprotein AI regulatory protein 1 antibody
    • ARP-1 antibody
    • ARP1 antibody
    • COT2_HUMAN antibody
    • COUP TF2 antibody
    • COUP transcription factor 2 antibody
    • COUP transcription factor II antibody
    • COUP-TF II antibody
    • COUP-TF2 antibody
    • NR2F2 antibody
    • Nuclear receptor subfamily 2 group F member 2 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR2F2 antibody [H7147] (ab41859)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR2F2 antibody [H7147] (ab41859)

    ab41859 staining human NR2F2 in convoluted tubule by Immunohistochemistry (FFPE-sections) 10ug/ml.

    This image was generated using the ascites version of the product.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR2F2 antibody [H7147] (ab41859)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR2F2 antibody [H7147] (ab41859)

    ab41859 staining rat NR2F2 in Glomerular by Immunohistochemistry (FFPE-sections) 10ug/ml.

    This image was generated using the ascites version of the product.

  • Western blot - Anti-NR2F2 antibody [H7147] (ab41859)
    Western blot - Anti-NR2F2 antibody [H7147] (ab41859)
    All lanes : Anti-NR2F2 antibody [H7147] (ab41859) at 1 µg/ml

    Lane 1 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 46 kDa
    Observed band size: 49 kDa why is the actual band size different from the predicted?
    Additional bands at: 72 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 4 minutes


    This image was generated using the ascites version of the product.

References (23)

Publishing research using ab41859? Please let us know so that we can cite the reference in this datasheet.

ab41859 has been referenced in 23 publications.

  • Polvani S  et al. Isoforms of the orphan nuclear receptor COUP‑TFII differentially modulate pancreatic cancer progression. Int J Oncol 60:N/A (2022). PubMed: 35348189
  • Meçe O  et al. Lipid droplet degradation by autophagy connects mitochondria metabolism to Prox1-driven expression of lymphatic genes and lymphangiogenesis. Nat Commun 13:2760 (2022). PubMed: 35589749
  • Wei JR  et al. Identification of visual cortex cell types and species differences using single-cell RNA sequencing. Nat Commun 13:6902 (2022). PubMed: 36371428
  • O'Leary TP  et al. Neuronal cell types, projections, and spatial organization of the central amygdala. iScience 25:105497 (2022). PubMed: 36425768
  • Aguiari P  et al. Persistent COUP-TFII expression underlies the myopathy and impaired muscle regeneration observed in resistance to thyroid hormone-alpha. Sci Rep 11:4601 (2021). PubMed: 33633251
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
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1-4 of 4 Abreviews or Q&A

Immunocytochemistry abreview for Anti-NR2F2 antibody [H7147]

 Average
Abreviews
abreview image
Application
Immunocytochemistry
Sample
Mouse Cell (BRAIN)
Permeabilization
Yes - 4% paraform
Specification
BRAIN
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Kyungjoo Seong

Verified customer

Submitted Jun 20 2017

Immunocytochemistry/ Immunofluorescence abreview for Anti-NR2F2 antibody [H7147]

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (human pluripotent stem cell derived cardiomyocytes)
Permeabilization
Yes - saponin
Specification
human pluripotent stem cell derived cardiomyocytes
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Aug 20 2015

Question

Dear xxxx,

Thank you for the answer. Yes, I would like to test ab41859 in ChIP experiments and would like to make a purchase of it. It would be grateful, if you could advise more on this, enroll us in the AbTrial testing program and issue me with a PO for the same. Alternatively, Kindly provide me with a local dealer contact in order to request a quote directly. Since, its quite urgent, I would like to make the order at the earliest.

Best regards

Read More
Answer

Thank you for your reply.
xxxx is currently away from the office but I will try to help with your request.
xxxx
I am very pleased to hear you would like to accept our offer and test ab41859 in ChIP. This code will give you €370 off your next order before the expiration date. To redeem this offer, please submit an Abreview for ChIP and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.

For more information on how to submit an Abreview, please visit the site:
www.abcam.com/Abreviews
Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways:

1) Call to place your order and mention the code to our customer service department;
2) Include the code in your fax order;
3) Place your order on the web and enter the promotional code.
Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.
The terms and conditions applicable to this offer can be found here:
www.abcam.com/abtrial

Read More

Question

Inquiry: I would like to know, if this product can be used for 'ChIP' experiments and also would like to order this antibody. kindly provide me with a quote. Thanks

Read More
Answer

Thank you for your enquiry.

I am sorry to confirm that as far as we are aware, ab41859 NR2F2 antibody [H7147] has never been tested in ChIP. All tested applications are specified on our datasheets, and these are updated as soon as any new information is brought to our attention.

Prices are provided on the individual datasheets. Select the correct country from the dropdown menu at the top right corner of the page, and the correct price and shipping costs for that country will show at the top of the datasheet.

ab41859 Price for Italy: 100 µl €370.00
Shipping: €29.00 to Italy

If you would like to test ab41859 in ChIP, please do not hesitate to contact me again prior to the purchase by replying to this message as you may be eligible for our AbTrial testing program. Please see the following page from our website for more information:

https://www.abcam.com/index.html?pageconfig=resource&rid=11998&source=pagetrap&viapagetrap=abtrial

Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

To find out more about our Abreview system, please see the following link:
https://www.abcam.com/abreviews

I hope this information is helpful. Please do not hesitate to contact me for any further advice or information.

Read More

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