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    products/primary-antibodies/nrf2-phospho-s40-antibody-ep1809y-ab76026.pdf

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RecombinantRabMAb

Recombinant Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)

  • Datasheet
  • SDS
Reviews (18)Q&A (14)References (139)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
  • Immunocytochemistry/ Immunofluorescence - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
  • Immunocytochemistry/ Immunofluorescence - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
  • Western blot - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
  • Immunocytochemistry/ Immunofluorescence - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
  • Flow Cytometry (Intracellular) - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
  • Dot Blot - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
  • Western blot - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
  • Western blot - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
  • Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1809Y] to Nrf2 (phospho S40)
  • Suitable for: Dot blot, ICC/IF, IHC-P, Flow Cyt (Intra), WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free

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Recombinant Human Nrf2 protein (ab132356)
Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Knockout
Product image
Human NFE2L2 (Nrf2) knockout HeLa cell line (ab262507)

View more associated products

Overview

  • Product name

    Anti-Nrf2 (phospho S40) antibody [EP1809Y]
    See all Nrf2 primary antibodies
  • Description

    Rabbit monoclonal [EP1809Y] to Nrf2 (phospho S40)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Dot blot, ICC/IF, IHC-P, Flow Cyt (Intra), WBmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
    (Peptide available as ab133404)

  • Positive control

    • WB: HepG2 whole cell lysate (ab7900). IHC-P: Human tonsil, breast carcinoma, ovarian carcinoma and cervical carcinoma tissue. ICC/IF: HepG2 cells. Flow Cyt (intra): Jurkat cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP1809Y
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Cell Biology
    • Other Antibodies
    • Oxidative Stress
    • Cardiovascular
    • Heart
    • Cardiac metabolism
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial Biogenesis
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Nucleotide metabolism
    • Molecular processes
    • Mitochondrial transcription
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Oxidative stress
    • Neuroscience
    • Development
    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • Anti-Nrf2 (phospho S40) antibody [EP1809Y] - BSA and Azide free (ab180844)
    • Alexa Fluor® 488 Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab237464)
    • Alexa Fluor® 647 Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab237465)
  • Assay kits

    • Nrf2 Transcription Factor Assay Kit (Colorimetric) (ab207223)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Hep G2 nuclear extract lysate (ab14660)
  • Recombinant Protein

    • Recombinant Human Nrf2 protein (ab132356)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab76026 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot
Use at an assay dependent concentration.
ICC/IF (1)
1/100 - 1/250.
IHC-P (3)
1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

Flow Cyt (Intra)
1/80 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB (13)
1/5000 - 1/50000. Predicted molecular weight: 68 kDa.
Notes
Dot blot
Use at an assay dependent concentration.
ICC/IF
1/100 - 1/250.
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

Flow Cyt (Intra)
1/80 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB
1/5000 - 1/50000. Predicted molecular weight: 68 kDa.
Application notes
Is unsuitable for IP.

Target

  • Function

    Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region.
  • Tissue specificity

    Widely expressed. Highest expression in adult muscle, kidney, lung, liver and in fetal muscle.
  • Sequence similarities

    Belongs to the bZIP family. CNC subfamily.
    Contains 1 bZIP domain.
  • Domain

    Acidic activation domain in the N-terminus, and DNA binding domain in the C-terminus.
  • Post-translational
    modifications

    Phosphorylation of Ser-40 by PKC in response to oxidative stress dissociates NFE2L2 from its cytoplasmic inhibitor KEAP1, promoting its translocation into the nucleus.
  • Cellular localization

    Cytoplasm > cytosol. Nucleus. Cytosolic under unstressed conditions, translocates into the nucleus upon induction by electrophilic agents.
  • Target information above from: UniProt accession Q16236 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 4780 Human
    • Omim: 600492 Human
    • SwissProt: Q16236 Human
    • Alternative names

      • erythroid derived 2 antibody
      • HEBP1 antibody
      • like 2 antibody
      • NF E2 related factor 2 antibody
      • NF-E2-related factor 2 antibody
      • NF2L2_HUMAN antibody
      • NFE2 related factor 2 antibody
      • NFE2-related factor 2 antibody
      • Nfe2l2 antibody
      • Nrf 2 antibody
      • NRF2 antibody
      • Nuclear factor (erythroid derived 2) like 2 antibody
      • Nuclear factor antibody
      • nuclear factor erythroid 2 like 2 antibody
      • Nuclear factor erythroid 2 related factor 2 antibody
      • Nuclear factor erythroid 2-related factor 2 antibody
      • Nuclear factor erythroid derived 2 like 2 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)Inui et al PLoS One. 2013 Sep 24;8(9):e74398. doi: 10.1371/journal.pone.0074398. eCollection 2013. Fig 2. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

      Nrf2 was abundantly expressed in carcinomas, low grade dysplasias, and non-atypical epithelia of oral tissue.

      Representative findings of Nrf2 staining in carcinoma (left), in low grade dysplasia (middle), and in non-atypical epithelium (right).

      Corresponding PLA signals are displayed in the lower row. Scale bar; 100 µm.

      Surgical specimens were transferred to 10% buffered formalin and fixed overnight. The fixed samples were embedded in paraffin, and serially sliced into 5 µm sections. After dewaxing, sections were autoclaved at 120°C for 1 min in 10 mM sodium citrate buffer (pH 6.0), and immersed in 0.3% H2O2. They were then incubated overnight at 4°C with primary antibody to Nrf2 (diluted 1:200). The sections were rinsed with 1×PBS and incubated with the secondary antibody conjugated with horseradish peroxidase at room temperature for 1 hour. The sections were then stained with 3.3′-diaminobenzidinetetrahydrochloride (DAB) and counterstained with hematoxylin.

    • Immunocytochemistry/ Immunofluorescence - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
      Immunocytochemistry/ Immunofluorescence - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)Giyshi et al PLoS Pathog. 2014 Oct 23;10(10):e1004460. doi: 10.1371/journal.ppat.1004460. eCollection 2014 Oct. Fig 1. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

      Immunofluorescence analysis of Nrf2 levels in Kaposi's sarcoma skin lesions.

      B) Healthy skin (top two rows) and KS skin tissue (bottom row) were double-stained for LANA-1 (Alexa-Fluor 594- red) and host phosphorylated pNrf2 (ab76026) (Alexa-Fluor®488 – green). DAPI was used to visualize the nuclei, and the triple merge of LANA-1, pNrf2 and DAPI is shown in the third column.

      Yellow square = enlarged area.

    • Immunocytochemistry/ Immunofluorescence - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
      Immunocytochemistry/ Immunofluorescence - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)Masuda et al PLoS One. 2015 Jun 25;10(6):e0131012. doi: 10.1371/journal.pone.0131012. eCollection 2015. Fig 1. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

      Nrf2 Translocation from cytoplasm to nucleus.

      (A) Human islets were treated with dh404 for 0.5, 1 or 2 hours. The treated and untreated samples were stained with Nrf2 antibody ab76026 (Green) and DAPI (Blue). The con-focal microscope clearly showed that the Nrf2 translocation from cytoplasm to nucleus in the dh404 treated human islet cells

    • Western blot - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
      Western blot - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
      All lanes : Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026) at 1/50000 dilution (purified)

      Lane 1 : untreated HepG2 cell lysate
      Lane 2 : HepG2 treated with phosphatase lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP goat anti-rabbit IgG (H+L) at 1/20000 dilution

      Predicted band size: 68 kDa
      Observed band size: 90 kDa why is the actual band size different from the predicted?



      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
      Immunohistochemical staining of paraffin embedded human breast carcinoma with purified ab76026 at a working dilution of 1/500. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
    • Immunocytochemistry/ Immunofluorescence - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
      Immunocytochemistry/ Immunofluorescence - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)

      Immunofluorescence staining of HepG2 cells with purified ab76026 at a working dilution of 1/100, counter-stained with DAPI. The treated cells were treated with alkaline phosphatase for 1 h at 37°C. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab76026 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

    • Flow Cytometry (Intracellular) - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
      Flow Cytometry (Intracellular) - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)

      Overlay histogram showing Jurkat cells fixed in 4% PFA and stained with purified ab76026 at a dilution of 1 in 80 (red line). The secondary antibody used was FITC goat anti-rabbit at a dilution of 1 in 500. Rabbit monoclonal IgG was used as an isotype control (black line) and cells incubated in the absence of both primary and secondary antibody were used as a negative control (blue line).

       

    • Dot Blot - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
      Dot Blot - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)

      Dot blot analysis of Nrf2 peptides using unpurified ab76026 at 1/1000 dilution followed by Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated secondary antibody at 1/1000 dilution. Blocking and diluting buffer was 5% NFDM/TBST.

       

      Lane 1: Nrf2 (pS40) phospho peptide

      Lane 2: Nrf2 non-phospho peptide

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)

      Immunohistochemical analysis of paraffin-embedded human breast carcinoma using unpurified ab76026 at 1/100 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Western blot - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
      Western blot - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
      All lanes : Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026) at 1/10000 dilution (unpurified)

      Lane 1 : Untreated HepG2 (human hepatocellular carcinoma) whole cell lysates 20µg
      Lane 2 : HepG2 (human hepatocellular carcinoma) treated with Alkaline Phosphatase (AP) whole cell lysates 20µg.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution

      Predicted band size: 68 kDa
      Observed band size: 100 kDa why is the actual band size different from the predicted?



      Blocking buffer: 5% NFDM/TBST, dilution buffer: 5% NFDM /TBST, exposure time: 15 seconds

    • Western blot - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
      Western blot - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
      All lanes : Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026) at 1/20000 dilution (unpurified)

      Lane 1 : HepG2 cell lysate
      Lane 2 : HepG2 cell lysate treated with AP

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/1000 dilution

      Predicted band size: 68 kDa
      Observed band size: 90 kDa why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)This image is courtesy of an anonymous Abreview

      Unpurified ab76026 staining Nrf2 (phospho S40) in Human normal lung tissue sections by IHC-P (Formaldehyde-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% casein for 30 minutes at 4°C. Antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/50) in 1% casein for 24 hours at 4°C. An undiluted HRP-conjugated Goat polyclonal to rabbit IgG was used as the secondary antibody.

      See Abreview

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)

      Unpurified ab76026 showing positive staining in Breast carcinoma tissue.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)

      Unpurified ab76026 showing positive staining in Cervical carcinoma tissue.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)

      Unpurified ab76026 showing positive staining in Ovarian carcinoma tissue.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)

      Unpurified ab76026 showing positive staining in Normal tonsil tissue.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)
      Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026)

    Protocols

    • Flow cytometry protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (139)

    Publishing research using ab76026? Please let us know so that we can cite the reference in this datasheet.

    ab76026 has been referenced in 139 publications.

    • Ho CC  et al. Extracts of Jasminum sambac flowers fermented by Lactobacillus rhamnosus inhibit H2 O2 - and UVB-induced aging in human dermal fibroblasts. Environ Toxicol 36:607-619 (2021). PubMed: 33270331
    • Hou X  et al. Hypoxia-reoxygenation treatment attenuates gestational diabetes mellitus. Endocr Connect 10:84-91 (2021). PubMed: 33320105
    • Li R  et al. Acidic fibroblast growth factor attenuates type 2 diabetes-induced demyelination via suppressing oxidative stress damage. Cell Death Dis 12:107 (2021). PubMed: 33479232
    • Zeng H  et al. Activated PKB/GSK-3ß synergizes with PKC-d signaling in attenuating myocardial ischemia/reperfusion injury via potentiation of NRF2 activity: Therapeutic efficacy of dihydrotanshinone-I. Acta Pharm Sin B 11:71-88 (2021). PubMed: 33532181
    • Zhou Y  et al. SIRT6 as a key event linking P53 and NRF2 counteracts APAP-induced hepatotoxicity through inhibiting oxidative stress and promoting hepatocyte proliferation. Acta Pharm Sin B 11:89-99 (2021). PubMed: 33532182
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 32 Abreviews or Q&A

    Western blot abreview for Anti-Nrf2 (phospho S40) antibody [EP1809Y]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Tissue lysate - whole (Skeletal muscle)
    Gel Running Conditions
    Reduced Denaturing (10-12.5% polyacrilamide gel Wet transfer at 400mA for 90 min.)
    Loading amount
    20 µg
    Specification
    Skeletal muscle
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 23°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Marcos Martin Rincon

    Verified customer

    Submitted Jul 25 2022

    Western blot abreview for Anti-Nrf2 (phospho S40) antibody [EP1809Y]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (differentiated neuroblastoma SHSY-5Y)
    Gel Running Conditions
    Reduced Denaturing (10%)
    Loading amount
    30 µg
    Treatment
    6OHDA w/ methionine, oxidized methionine or taurine
    Specification
    differentiated neuroblastoma SHSY-5Y
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    PROF. Michele D'angelo

    Verified customer

    Submitted Oct 01 2021

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Nrf2 (phospho S40) antibody [EP1809Y]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (SH-SY5YH)
    Permeabilization
    Yes - TRITON X-100 0.05% in PBS
    Specification
    SH-SY5YH
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted May 22 2020

    Western blot abreview for Anti-Nrf2 (phospho S40) antibody [EP1809Y]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (SH-SY5Y cells)
    Gel Running Conditions
    Reduced Denaturing (10%)
    Loading amount
    30 µg
    Treatment
    H2O2 50uM for 24 hrs
    Specification
    SH-SY5Y cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted May 13 2020

    Western blot abreview for Anti-Nrf2 (phospho S40) antibody [EP1809Y]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Primary Prostate Fibroblasts)
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Loading amount
    70 µg
    Treatment
    SOD
    Specification
    Primary Prostate Fibroblasts
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Elizabeth Kosmacek

    Verified customer

    Submitted Oct 25 2019

    Western blot abreview for Anti-Nrf2 (phospho S40) antibody [EP1809Y]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (BV2 microglia)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    70 µg
    Treatment
    5µM LPA for different time points
    Specification
    BV2 microglia
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Lisha Joshi

    Verified customer

    Submitted Jun 12 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Nrf2 (phospho S40) antibody [EP1809Y]

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (pancreas)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10 mM TRIS-EDTA pH 9 at 98°C for 20 minutes, normal pressure
    Permeabilization
    Yes - 0.04% triton 100 x in the blocking solution
    Specification
    pancreas
    Blocking step
    BSA as blocking agent for 15 minute(s) · Concentration: 2% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Dec 13 2016

    Western blot abreview for Anti-Nrf2 (phospho S40) antibody [EP1809Y]

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (lungs)
    Gel Running Conditions
    Reduced Denaturing (30 minutes RT, 10% gel)
    Loading amount
    100 µg
    Specification
    lungs
    Blocking step
    2 % Topblock in TBT-0.1% Triton 100 x as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Dec 13 2016

    Western blot abreview for Anti-Nrf2 (phospho S40) antibody [EP1809Y]

    Below Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (brain)
    Gel Running Conditions
    Reduced Denaturing (10)
    Loading amount
    50 µg
    Specification
    brain
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jul 12 2016

    Western blot abreview for Anti-Nrf2 (phospho S40) antibody [EP1809Y]

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Tissue lysate - whole (Brain)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    50 µg
    Specification
    Brain
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jul 12 2016

    1-10 of 32 Abreviews or Q&A

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