Recombinant Anti-NSG2 antibody [EPR15389(B)] - C-terminal (ab189513)
![Western blot - Anti-NSG2 antibody [EPR15389(B)] - C-terminal (ab189513)](https://www.abcam.com/ps/products/189/ab189513/Images/ab189513-225131-anti-nsg2-antibody-epr15389b-c-terminal-western-blot.jpg "Western blot - Anti-NSG2 antibody [EPR15389(B)] - C-terminal (ab189513)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15389(B)] to NSG2 - C-terminal
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-NSG2 antibody [EPR15389(B)] - C-terminal
See all NSG2 primary antibodies -
Description
Rabbit monoclonal [EPR15389(B)] to NSG2 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Neuro-2a, SH-SY5Y, Human fetal brain and rat brain lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15389(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab189513 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa).
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| Notes |
|---|
|
WB
1/1000. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa). |
Target
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Sequence similarities
Belongs to the NSG family. -
Cellular localization
Cytoplasmic vesicle membrane. Golgi apparatus > Golgi stack membrane. Endosome > multivesicular body membrane. Mainly Golgi stack, but also found in small vacuolar organelles and multivesicular bodies. - Information by UniProt
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Database links
- Entrez Gene: 51617 Human
- Entrez Gene: 18197 Mouse
- Entrez Gene: 497878 Rat
- SwissProt: Q9Y328 Human
- SwissProt: P47759 Mouse
- Unigene: 559412 Human
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Alternative names
- HMP19 antibody
- Neuron-specific protein family member 2 antibody
- Nsg2 antibody
see all
Images
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Western blot - Anti-NSG2 antibody [EPR15389(B)] - C-terminal (ab189513)All lanes : Anti-NSG2 antibody [EPR15389(B)] - C-terminal (ab189513) at 1/1000 dilution
Lane 1 : Neuro-2a cell lysate
Lane 2 : Human fetal brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 19 kDa -
![Western blot - Anti-NSG2 antibody [EPR15389(B)] - C-terminal (ab189513)](https://www.abcam.com/ps/products/189/ab189513/Images/ab189513-225132-anti-nsg2-antibody-epr15389b-c-terminal-western-blot.jpg)
Western blot - Anti-NSG2 antibody [EPR15389(B)] - C-terminal (ab189513)All lanes : Anti-NSG2 antibody [EPR15389(B)] - C-terminal (ab189513) at 1/1000 dilution
Lane 1 : SH-SY5Y cell lysate
Lane 2 : Rat brain lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 19 kDa -
![Anti-NSG2 antibody [EPR15389(B)] - C-terminal (ab189513)](https://www.abcam.com/ps/products/189/ab189513/Images/ab189513-3-benefits-of-recombinant-antibodies.png)
Anti-NSG2 antibody [EPR15389(B)] - C-terminal (ab189513)
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (4)
ab189513 has been referenced in 4 publications.
- Yap CC et al. Dynein Is Required for Rab7-Dependent Endosome Maturation, Retrograde Dendritic Transport, and Degradation. J Neurosci 42:4415-4434 (2022). PubMed: 35474277
- Austin R et al. Global loss of Neuron-specific gene 1 causes alterations in motor coordination, increased anxiety, and diurnal hyperactivity in male mice. Genes Brain Behav 21:e12816 (2022). PubMed: 35577358
- Chander P et al. Neuron-Specific Gene 2 (NSG2) Encodes an AMPA Receptor Interacting Protein That Modulates Excitatory Neurotransmission. eNeuro 6:N/A (2019). PubMed: 30680309
- Barford K et al. The related neuronal endosomal proteins NEEP21 (Nsg1) and P19 (Nsg2) have divergent expression profiles in vivo. J Comp Neurol 525:1861-1878 (2017). PubMed: 28299779