Anti-Nuclear Receptor Corepressor NCoR antibody (ab58396)
Key features and details
- Rabbit polyclonal to Nuclear Receptor Corepressor NCoR
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Nuclear Receptor Corepressor NCoR antibody
See all Nuclear Receptor Corepressor NCoR primary antibodies -
Description
Rabbit polyclonal to Nuclear Receptor Corepressor NCoR -
Host species
Rabbit -
Specificity
This antibody detects endogenous levels of total NCoR1 protein. -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide derived from the internal sequence of Nuclear Receptor Corepressor NCoR (Human)
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg+2 and Ca+2 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from rabbit antiserum. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
- Signal Transduction
- Signaling Pathway
- Nuclear Signaling
- Nuclear Hormone Receptors
- Co-activators/co-repressors
- Epigenetics and Nuclear Signaling
- Nuclear Signaling Pathways
- Nuclear Receptors
- Co-activators/co-repressors
- Epigenetics and Nuclear Signaling
- Chromatin Modifying Enzymes
- Acetylation
- HDACs
- Class II / Hda1 Class
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab58396 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Predicted molecular weight: 270 kDa.
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IHC-P |
1/50 - 1/100.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 270 kDa. |
IHC-P
1/50 - 1/100. |
Target
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Function
Mediates transcriptional repression by certain nuclear receptors. Part of a complex which promotes histone deacetylation and the formation of repressive chromatin structures which may impede the access of basal transcription factors. -
Sequence similarities
Belongs to the N-CoR nuclear receptor corepressors family.
Contains 2 SANT domains. -
Domain
The N-terminal region contains three independent domains that are capable of mediating transcriptional repression (RD1, RD2 and RD3).
The C-terminal region contains two separate nuclear receptor-interacting domains (ID1 and ID2), each of which contains a conserved sequence referred to as the CORNR box. This motif is necessary and sufficient for binding to unligated nuclear hormone receptors, while sequences flanking the CORNR box determine the precise nuclear hormone receptor specificity. -
Post-translational
modificationsUbiquitinated; mediated by SIAH2 and leading to its subsequent proteasomal degradation. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 9611 Human
- Omim: 600849 Human
- SwissProt: O75376 Human
- Unigene: 462323 Human
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Alternative names
- hN CoR antibody
- hNCoR antibody
- KIAA1047 antibody
see all
Images
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All lanes : Anti-Nuclear Receptor Corepressor NCoR antibody (ab58396) at 1/500 dilution
Lane 1 : MDA-MB-435 cell extract with no immunizing peptide
Lane 2 : MDA-MB-435 cell extract with immunizing peptide
Predicted band size: 270 kDa -
ab58396, at 1/50 diltuion, staining human Nuclear Receptor Corepressor NCoR in breast carcinoma tissue by immunohistochemistry in the presence or absence of the immunizing peptide. Paraffin embedded tissue.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab58396 has been referenced in 5 publications.
- Wang Y et al. Discovery of LAMP-2A as potential biomarkers for glioblastoma development by modulating apoptosis through N-CoR degradation. Cell Commun Signal 19:40 (2021). PubMed: 33761934
- Chen FX et al. Intramuscular accumulation of pentadecanoic acid activates AKT1 to phosphorylate NCOR1 and triggers FOXM1-mediated apoptosis in the pathogenesis of sarcopenia. Am J Transl Res 12:5064-5079 (2020). PubMed: 33042406
- Figueroa J et al. Exosomes from Glioma-Associated Mesenchymal Stem Cells Increase the Tumorigenicity of Glioma Stem-like Cells via Transfer of miR-1587. Cancer Res 77:5808-5819 (2017). PubMed: 28855213
- Gallardo F et al. Cytoplasmic accumulation of NCoR in malignant melanoma: consequences of altered gene repression and prognostic significance. Oncotarget 6:9284-94 (2015). WB ; Human . PubMed: 25823659
- Scaffidi P & Misteli T Lamin A-dependent misregulation of adult stem cells associated with accelerated ageing. Nat Cell Biol 10:452-9 (2008). ICC/IF ; Human . PubMed: 18311132