Anti-Nucleolin antibody [364-5] (ab136649)
![Immunocytochemistry/ Immunofluorescence - Anti-Nucleolin antibody [364-5] (ab136649)](https://www.abcam.com/ps/products/136/ab136649/Images/ab136649-219553-anti-nucleolin-antibody-364-5-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Nucleolin antibody [364-5] (ab136649)")
Key features and details
- Mouse monoclonal [364-5] to Nucleolin
- Suitable for: ICC/IF, IHC-P, WB, IP, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
Anti-Nucleolin antibody [364-5]
See all Nucleolin primary antibodies -
Description
Mouse monoclonal [364-5] to Nucleolin -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, IHC-P, WB, IP, Flow Cytmore details -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat, Cow -
Immunogen
Tissue, cells or virus. This information is considered to be commercially sensitive.
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Positive control
- HeLa and HDFn cells; HepG2, HDFn and SH-SY5Y whole cell lysates.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.88% Sodium chloride, 0.36% HEPES -
Concentration information loading... -
Purification notes
ab136649 is purified by biochemical fractionation. Near homogeneity as judged by SDS-PAGE. -
Clonality
Monoclonal -
Clone number
364-5 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab136649 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | (2) |
Use a concentration of 1 µg/ml.
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| IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 76 kDa.
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| IP |
Use a concentration of 1 µg/ml.
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| Flow Cyt |
Use a concentration of 1 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
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ICC/IF
Use a concentration of 1 µg/ml. |
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
|
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 76 kDa. |
|
IP
Use a concentration of 1 µg/ml. |
|
Flow Cyt
Use a concentration of 1 µg/ml. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Function
Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats. -
Sequence similarities
Contains 4 RRM (RNA recognition motif) domains. -
Post-translational
modificationsSome glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group. -
Cellular localization
Nucleus > nucleolus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. - Information by UniProt
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Database links
- Entrez Gene: 4691 Human
- Omim: 164035 Human
- SwissProt: P19338 Human
- Unigene: 79110 Human
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Alternative names
- C23 antibody
- FLJ45706 antibody
- MS1116 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Nucleolin antibody [364-5] (ab136649)This image is courtesy of an anonymous Abreview
ab136649 staining Nucleolin in human breast cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 5% Triton X-100 in PBS and blocked with 5% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/1000) for 1 hour. An undiluted Alexa Fluor® 546-conjugated anti-mouse IgG polyclonal was used as the secondary antibody.
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![Western blot - Anti-Nucleolin antibody [364-5] (ab136649)](https://www.abcam.com/ps/products/136/ab136649/Images/ab136649-151220-anti-nucleolin-antibody-364-5-western-blot.jpg)
Western blot - Anti-Nucleolin antibody [364-5] (ab136649)All lanes : Anti-Nucleolin antibody [364-5] (ab136649) at 1 µg/ml
Lane 1 : HepG2 whole cell lysate at 20 µg
Lane 2 : HDFn whole cell lysate at 25 µg
Lane 3 : SH-SY5Y whole cell lysate at 20 µg
Secondary
All lanes : Goat polyclonal to Mouse IgG - AP at 1/3000 dilution
Predicted band size: 76 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-Nucleolin antibody [364-5] (ab136649)](https://www.abcam.com/ps/products/136/ab136649/Images/ab136649-151219-anti-nucleolin-antibody-364-5-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Nucleolin antibody [364-5] (ab136649)Immunocytochemical analysis of HeLa cells (paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15 min)) labelling Nucleolin with ab136649 at 1µg/ml (red) and anti-GAPDH (ab110305) in green, as a counter stain.
Secondary: Alexa 594 goat anti-mouse IgG1 at a 1/1000 dilution. -
![Immunocytochemistry/ Immunofluorescence - Anti-Nucleolin antibody [364-5] (ab136649)](https://www.abcam.com/ps/products/136/ab136649/Images/ab136649-151218-anti-nucleolin-antibody-364-5-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Nucleolin antibody [364-5] (ab136649)Immunocytochemical analysis of HDFn cells (paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15 min)) labelling Nucleolin with ab136649 at 1µg/ml (red) and anti-GAPDH (ab110305) in green, as a counter stain. Secondary: Alexa 594 goat anti-mouse IgG1 at a 1/1000 dilution. -
![Immunoprecipitation - Anti-Nucleolin antibody [364-5] (ab136649)](https://www.abcam.com/ps/products/136/ab136649/Images/ab136649-151217-anti-nucleolin-antibody-364-5-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Nucleolin antibody [364-5] (ab136649)Detection of Nucleolin by Immunoprecipitation.
Nucleolin in HepG2 cell lysate (1 mg) was immunoprecipitated using 1 µg ab136649 antibody. The gel was stained with Coomassie brilliant blue G. -
![Flow Cytometry - Anti-Nucleolin antibody [364-5] (ab136649)](https://www.abcam.com/ps/products/136/ab136649/Images/ab136649-151216-anti-nucleolin-antibody-364-5-flow-cytometry.jpg)
Flow Cytometry - Anti-Nucleolin antibody [364-5] (ab136649)Flow Cytometric analysis of HeLa cells (fixed and permeabilized with methanol) labelling Nucleolin with ab136649 (blue) at 1µg/ml or an equal amount of an isotype control antibody (red). -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin antibody [364-5] (ab136649)](https://www.abcam.com/ps/products/136/ab136649/Images/ab136649-151215-anti-nucleolin-antibody-364-5-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin antibody [364-5] (ab136649)IHC image of nucleolin staining in human liver HCC formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab136649, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab136649 has been referenced in 9 publications.
- Li F et al. The Atr-Chek1 pathway inhibits axon regeneration in response to Piezo-dependent mechanosensation. Nat Commun 12:3845 (2021). PubMed: 34158506
- Nihei Y et al. Poly-glycine-alanine exacerbates C9orf72 repeat expansion-mediated DNA damage via sequestration of phosphorylated ATM and loss of nuclear hnRNPA3. Acta Neuropathol 139:99-118 (2020). PubMed: 31642962
- Dubois ML et al. UBB pseudogene 4 encodes functional ubiquitin variants. Nat Commun 11:1306 (2020). PubMed: 32161257
- Logan NJ et al. Demethylation of ITGAV accelerates osteogenic differentiation in a blast-induced heterotopic ossification in vitro cell culture model. Bone 117:149-160 (2018). PubMed: 30219480
- Son A et al. PARN and TOE1 Constitute a 3' End Maturation Module for Nuclear Non-coding RNAs. Cell Rep 23:888-898 (2018). ICC/IF ; Human . PubMed: 29669292
- Gao Z et al. The PA-interacting host protein nucleolin acts as an antiviral factor during highly pathogenic H5N1 avian influenza virus infection. Arch Virol 163:2775-2786 (2018). PubMed: 29974255
- Stopford MJ et al. C9ORF72 hexanucleotide repeat exerts toxicity in a stable, inducible motor neuronal cell model, which is rescued by partial depletion of Pten. Hum Mol Genet 26:1133-1145 (2017). ICC/IF ; Mouse . PubMed: 28158451
- Kohli JS et al. ETS1, nucleolar and non-nucleolar TERT expression in nevus to melanoma progression. Oncotarget 8:104408-104417 (2017). PubMed: 29262649
- Mori K et al. Reduced hnRNPA3 increases C9orf72 repeat RNA levels and dipeptide-repeat protein deposition. EMBO Rep 17:1314-25 (2016). PubMed: 27461252