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    products/primary-antibodies/nudt19-antibody-epr13162-106-ab180167.pdf

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Signal Transduction Second Messenger Lipid Messengers
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RecombinantRabMAb

Recombinant Anti-NUDT19 antibody [EPR13162-106] (ab180167)

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Western blot - Anti-NUDT19 antibody [EPR13162-106] (ab180167)
  • Immunocytochemistry/ Immunofluorescence - Anti-NUDT19 antibody [EPR13162-106] (ab180167)
  • Anti-NUDT19 antibody [EPR13162-106] (ab180167)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13162-106] to NUDT19
  • Suitable for: WB, ICC/IF
  • Reacts with: Human

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Overview

  • Product name

    Anti-NUDT19 antibody [EPR13162-106]
    See all NUDT19 primary antibodies
  • Description

    Rabbit monoclonal [EPR13162-106] to NUDT19
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment within Human NUDT19. The exact sequence is proprietary.
    Database link: A8MXV4

  • Positive control

    • K562, 293T, Human ovary and Human spleen lysates; 293T cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR13162-106
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Second Messenger
    • Lipid Messengers
    • Signal Transduction
    • Metabolism
    • Lipid metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Hydrolysis
    • Metabolism
    • Pathways and Processes
    • Cofactors, Vitamins / minerals
    • Co-factors

Associated products

  • Alternative Versions

    • Anti-NUDT19 antibody [EPR13162-106] - BSA and Azide free (ab250171)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • K-562 whole cell lysate (ab7911)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab180167 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/5000. Predicted molecular weight: 42 kDa.
ICC/IF
1/50 - 1/100.
Notes
WB
1/1000 - 1/5000. Predicted molecular weight: 42 kDa.
ICC/IF
1/50 - 1/100.
Application notes
Is unsuitable for Flow Cyt,IHC-P or IP.

Target

  • Function

    Coenzyme A diphosphatase that mediates the hydrolysis of a wide range of CoA esters, including choloyl-CoA and branched-chain fatty-acyl-CoA esters. At low substrate concentrations medium and long-chain fatty-acyl-CoA esters are the primary substrates.
  • Sequence similarities

    Belongs to the Nudix hydrolase family.
    Contains 1 nudix hydrolase domain.
  • Cellular localization

    Peroxisome. Mitochondrion.
  • Target information above from: UniProt accession A8MXV4 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 390916 Human
    • Entrez Gene: 110959 Mouse
    • Entrez Gene: 308518 Rat
    • SwissProt: A8MXV4 Human
    • SwissProt: P11930 Mouse
    • SwissProt: Q6AYD9 Rat
    • Unigene: 203961 Human
    • Alternative names

      • mitochondrial antibody
      • Nucleoside diphosphate-linked moiety X motif 19 antibody
      • nucleoside diphosphate-linked moiety X motif 19, mitochondrial antibody
      • NUD19_HUMAN antibody
      • nudix (nucleoside diphosphate linked moiety X)-type motif 19 antibody
      • Nudix motif 19 antibody
      • NUDT19 antibody
      • RP2 antibody
      see all

    Images

    • Western blot - Anti-NUDT19 antibody [EPR13162-106] (ab180167)
      Western blot - Anti-NUDT19 antibody [EPR13162-106] (ab180167)
      All lanes : Anti-NUDT19 antibody [EPR13162-106] (ab180167) at 1/1000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : Human ovary lysate
      Lane 4 : Human spleen lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Standard HRP labeled goat anti-rabbit at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 42 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-NUDT19 antibody [EPR13162-106] (ab180167)
      Immunocytochemistry/ Immunofluorescence - Anti-NUDT19 antibody [EPR13162-106] (ab180167)

      Immunofluorescent analysis of 293T cells labeling NUDT19 with ab180167 at 1/50 dilution (green). DAPI nuclear staining (blue).

    • Anti-NUDT19 antibody [EPR13162-106] (ab180167)
      Anti-NUDT19 antibody [EPR13162-106] (ab180167)

    Protocols

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab180167? Please let us know so that we can cite the reference in this datasheet.

    ab180167 has not yet been referenced specifically in any publications.

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