Recombinant Anti-NUDT19 antibody [EPR13162-63] (ab180166)
![Western blot - Anti-NUDT19 antibody [EPR13162-63] (ab180166)](https://www.abcam.com/ps/products/180/ab180166/Images/ab180166-208732-anti-nudt19-antibody-epr13162-63-western-blot.jpg "Western blot - Anti-NUDT19 antibody [EPR13162-63] (ab180166)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13162-63] to NUDT19
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-NUDT19 antibody [EPR13162-63]
See all NUDT19 primary antibodies -
Description
Rabbit monoclonal [EPR13162-63] to NUDT19 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human NUDT19. The exact sequence is proprietary.
Database link: A8MXV4 -
Positive control
- Human heart and kidney tissues. K562, 293T, Human ovary and Human spleen lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13162-63 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab180166 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/100 - 1/250.
|
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| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
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| WB |
1/1000 - 1/10000. Predicted molecular weight: 42 kDa.
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| Notes |
|---|
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ICC/IF
1/100 - 1/250. |
|
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
1/1000 - 1/10000. Predicted molecular weight: 42 kDa. |
Target
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Function
Coenzyme A diphosphatase that mediates the hydrolysis of a wide range of CoA esters, including choloyl-CoA and branched-chain fatty-acyl-CoA esters. At low substrate concentrations medium and long-chain fatty-acyl-CoA esters are the primary substrates. -
Sequence similarities
Belongs to the Nudix hydrolase family.
Contains 1 nudix hydrolase domain. -
Cellular localization
Peroxisome. Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 390916 Human
- SwissProt: A8MXV4 Human
- Unigene: 203961 Human
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Alternative names
- mitochondrial antibody
- Nucleoside diphosphate-linked moiety X motif 19 antibody
- nucleoside diphosphate-linked moiety X motif 19, mitochondrial antibody
see all
Images
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Western blot - Anti-NUDT19 antibody [EPR13162-63] (ab180166)All lanes : Anti-NUDT19 antibody [EPR13162-63] (ab180166) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Human ovary tissue lysate
Lane 4 : Human spleen tissue lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Predicted band size: 42 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUDT19 antibody [EPR13162-63] (ab180166)](https://www.abcam.com/ps/products/180/ab180166/Images/ab180166-208731-anti-nudt19-antibody-epr13162-63-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUDT19 antibody [EPR13162-63] (ab180166)Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling NUDT19 using ab180166 at 1/50.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUDT19 antibody [EPR13162-63] (ab180166)](https://www.abcam.com/ps/products/180/ab180166/Images/ab180166-208729-anti-nudt19-antibody-epr13162-63-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUDT19 antibody [EPR13162-63] (ab180166)Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling NUDT19 using ab180166 at 1/50.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-NUDT19 antibody [EPR13162-63] (ab180166)](https://www.abcam.com/ps/products/180/ab180166/Images/ab180166-208728-anti-nudt19-antibody-epr13162-63-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-NUDT19 antibody [EPR13162-63] (ab180166)Immunofluorescence analysis of 293T cells labeling NUDT19 using ab180166 at 1/100 (green). DAPI nuclear staining (blue).
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![Anti-NUDT19 antibody [EPR13162-63] (ab180166)](https://www.abcam.com/ps/products/180/ab180166/Images/ab180166-5-benefits-of-recombinant-antibodies.png)
Anti-NUDT19 antibody [EPR13162-63] (ab180166)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab180166 has not yet been referenced specifically in any publications.