Recombinant Anti-NUP133 antibody [EPR10808(B)] (ab155990)
![Immunocytochemistry/ Immunofluorescence - Anti-NUP133 antibody [EPR10808(B)] (ab155990)](https://www.abcam.com/ps/products/155/ab155990/Images/ab155990-307094-anti-nup133-antibody-epr10808b-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-NUP133 antibody [EPR10808(B)] (ab155990)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10808(B)] to NUP133
- Suitable for: WB, ICC/IF
- Reacts with: Human, African green monkey
Related conjugates and formulations
Overview
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Product name
Anti-NUP133 antibody [EPR10808(B)]
See all NUP133 primary antibodies -
Description
Rabbit monoclonal [EPR10808(B)] to NUP133 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: Flow Cyt or IHC-P -
Species reactivity
Reacts with: Human, African green monkey
Predicted to work with: Monkey
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa, Ramos and SH-SY5Y cell lysates; HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR10808(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab155990 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 129 kDa.
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| ICC/IF | (1) |
1/50 - 1/100.
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| Notes |
|---|
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WB
1/1000 - 1/10000. Predicted molecular weight: 129 kDa. |
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ICC/IF
1/50 - 1/100. |
Target
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Function
Involved in poly(A)+ RNA transport. -
Sequence similarities
Belongs to the nucleoporin Nup133 family. -
Cellular localization
Nucleus > nuclear pore complex. Chromosome > centromere > kinetochore. Located on both the cytoplasmic and nuclear sides of the nuclear pore. During mitosis, localizes to the kinetochores. - Information by UniProt
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Database links
- Entrez Gene: 55746 Human
- Omim: 607613 Human
- SwissProt: Q8WUM0 Human
- Unigene: 12457 Human
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Alternative names
- 133 kDa nucleoporin antibody
- FLJ10814 antibody
- hNUP 133 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-NUP133 antibody [EPR10808(B)] (ab155990)
Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labeling NUP133 with purified ab155990 at 1:50 (6.5 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 μg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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![Western blot - Anti-NUP133 antibody [EPR10808(B)] (ab155990)](https://www.abcam.com/ps/products/155/ab155990/Images/ab155990-307074-anti-nup133-antibody-epr10808b-western-blot.jpg)
Western blot - Anti-NUP133 antibody [EPR10808(B)] (ab155990)Anti-NUP133 antibody [EPR10808(B)] (ab155990) at 1/1000 dilution (purified) + COS-1 (African green monkey kidney fibroblast-like) whole cell lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 129 kDa
Observed band size: 129 kDa -
![Western blot - Anti-NUP133 antibody [EPR10808(B)] (ab155990)](https://www.abcam.com/ps/products/155/ab155990/Images/ab155990-307073-anti-nup133-antibody-epr10808b-western-blot.jpg)
Western blot - Anti-NUP133 antibody [EPR10808(B)] (ab155990)Lanes 1 & 3 : Anti-NUP133 antibody [EPR10808(B)] (ab155990) at 1/3000 dilution (purified)
Lane 2 : Anti-NUP133 antibody [EPR10808(B)] (ab155990) at 1/3000 dilution (purifed)
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : Ramos (Human Burkitt's lymphoma B lymphocyte) whole cell lysates
Lane 3 : SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 129 kDa
Observed band size: 129 kDa -
![Western blot - Anti-NUP133 antibody [EPR10808(B)] (ab155990)](https://www.abcam.com/ps/products/155/ab155990/Images/ab155990-163312-anti-nup133-antibody-epr10808b-western-blot.jpg)
Western blot - Anti-NUP133 antibody [EPR10808(B)] (ab155990)All lanes : Anti-NUP133 antibody [EPR10808(B)] (ab155990) at 1/1000 dilution (unpurified)
Lane 1 : HeLa cell lysate
Lane 2 : Ramos cell lysate
Lane 3 : SH-SY5Y cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 129 kDaThis image was produced using unpurified antibody.
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![Immunocytochemistry/ Immunofluorescence - Anti-NUP133 antibody [EPR10808(B)] (ab155990)](https://www.abcam.com/ps/products/155/ab155990/Images/ab155990-233390-anti-nup133-antibody-epr10808b-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-NUP133 antibody [EPR10808(B)] (ab155990)This image is courtesy of an Abreview submitted by Aaron HalpenUnpurified ab155990 staining NUP133 in monkey kidney cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 3% BSA + 0.5% Triton X-100 for 45 minutes at 25°C. Samples were incubated with primary antibody (1/1500 in 3% BSA + 0.5% Triton X-100) for 45 minutes at 25°C. An Alexa Fluor® 647-conjugated donkey anti-rabbit IgG polyclonal (2 µg/ml) was used as the secondary antibody. This image was produced using unpurified antibody.
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![Immunocytochemistry/ Immunofluorescence - Anti-NUP133 antibody [EPR10808(B)] (ab155990)](https://www.abcam.com/ps/products/155/ab155990/Images/ab155990-163311-anti-nup133-antibody-epr10808b-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-NUP133 antibody [EPR10808(B)] (ab155990)Immunofluorescent analysis of HeLa cells labeling NUP133 with unpurified ab155990 at 1/50 dilution. This image was produced using unpurified antibody.
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![Anti-NUP133 antibody [EPR10808(B)] (ab155990)](https://www.abcam.com/ps/products/155/ab155990/Images/ab155990-7-benefits-of-recombinant-antibodies.png)
Anti-NUP133 antibody [EPR10808(B)] (ab155990)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab155990 has been referenced in 7 publications.
- Mammel AE et al. Chromosome length and gene density contribute to micronuclear membrane stability. Life Sci Alliance 5:N/A (2022). PubMed: 34789512
- Chachoua I et al. Canonical WNT signaling-dependent gating of MYC requires a noncanonical CTCF function at a distal binding site. Nat Commun 13:204 (2022). PubMed: 35017527
- Chou YY et al. Inherited nuclear pore substructures template post-mitotic pore assembly. Dev Cell 56:1786-1803.e9 (2021). PubMed: 34129835
- Bilir S et al. Roles of Nup133, Nup153 and membrane fenestrations in assembly of the nuclear pore complex at the end of mitosis. Genes Cells 24:338-353 (2019). PubMed: 30821042
- Platani M et al. Seh1 targets GATOR2 and Nup153 to mitotic chromosomes. J Cell Sci 131:N/A (2018). PubMed: 29618633
- Aristov A et al. ZOLA-3D allows flexible 3D localization microscopy over an adjustable axial range. Nat Commun 9:2409 (2018). PubMed: 29921892
- Liu S et al. Nuclear envelope assembly defects link mitotic errors to chromothripsis. Nature 561:551-555 (2018). PubMed: 30232450