Anti-OATP1A2 antibody (ab221804)
Key features and details
- Rabbit polyclonal to OATP1A2
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-OATP1A2 antibody
See all OATP1A2 primary antibodies -
Description
Rabbit polyclonal to OATP1A2 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human OATP1A2 aa 250-350.
Database link: P46721 -
Positive control
- ICC/IF: SH-SY5Y cells. WB: RT4 and U-251 MG cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab221804 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
|
| WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 74 kDa.
|
| Notes |
|---|
|
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 74 kDa. |
Target
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Function
Mediates the Na(+)-independent transport of organic anions such as sulfobromophthalein (BSP) and conjugated (taurocholate) and unconjugated (cholate) bile acids (By similarity). Selectively inhibited by the grapefruit juice component naringin. -
Sequence similarities
Belongs to the organo anion transporter (TC 2.A.60) family.
Contains 1 Kazal-like domain. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 6579 Human
- Omim: 602883 Human
- SwissProt: P46721 Human
- Unigene: 46440 Human
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Alternative names
- OATP antibody
- OATP-1 antibody
- OATP-A antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-OATP1A2 antibody (ab221804)
PFA-fixed, Triton X-100 permeabilized SH-SY5Y (human neuroblastoma cell line from bone marrow) cells stained for OATP1A2 (green) using ab221804 at 4 µg/ml in ICC/IF.
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Western blot - Anti-OATP1A2 antibody (ab221804)Anti-OATP1A2 antibody (ab221804) at 0.4 µg/ml (NTERA-2 cell lysate)
Predicted band size: 74 kDaWestern blot analysis of NTERA-2 cell lysate with ab221804 at 0.4 µg/ml.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab221804 has not yet been referenced specifically in any publications.