Name: Anti-Occludin antibody [EPR20992]
Brand: RabMAb
SKU: ab216327
Rating: 4 (7 reviews)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody [EPR20992] (ab216327)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR20992] to Occludin
Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cyt (Intra)
Knockout validated
Reacts with: Mouse, Rat, Dog, Human

Carrier Free

Product name

Anti-Occludin antibody [EPR20992]
See all Occludin primary antibodies
Description

Rabbit monoclonal [EPR20992] to Occludin
Host species

Rabbit
Specificity

This antibody is not suitable for ICC in mouse species

Not suitable for testing rat intestinal tissues
Tested applications

Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cyt (Intra)more details
Species reactivity

Reacts with: Mouse, Rat, Dog, Human
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human colon lysate; MDCK, PC-3, HEK-293 and Caco-2 whole cell lysates; Rat brain and lung lysate; Mouse colon, brain, lung and uterus lysates; bEnd.3 whole cell lysate. IHC-P: Human colon and breast tissues; human thyroid carcinoma; Mouse and rat kidney tissues. ICC/IF: Caco-2 and MDCK cells. Flow Cyt (intra): Caco-2 cells. IP: Caco-2 whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol, PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR20992
Isotype

IgG
Research areas
- Neuroscience
- Development

Alternative Versions
- Anti-Occludin antibody [EPR20992] - BSA and Azide free (ab224526)
Compatible Secondaries
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Recombinant Protein
- Recombinant Human Occludin protein (ab114189)
Related Products
- Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889)
- Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab216327 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB	(3)	1/1000. Detects a band of approximately 65, 53, 25, 23 kDa (predicted molecular weight: 59 kDa). Please upload more of the lysate or use lower dilution of ab216327 when testing bEnd.3 and mouse intestinal tissues. ab216327 is not suitable for testing rat intestinal tissues.
IHC-P	(3)	1/200. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF		1/100. This antibody is not suitable for ICC in mouse species.
IP		1/40.
Flow Cyt (Intra)		1/60.

Notes
WB 1/1000. Detects a band of approximately 65, 53, 25, 23 kDa (predicted molecular weight: 59 kDa). Please upload more of the lysate or use lower dilution of ab216327 when testing bEnd.3 and mouse intestinal tissues. ab216327 is not suitable for testing rat intestinal tissues.
IHC-P 1/200. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/100. This antibody is not suitable for ICC in mouse species.
IP 1/40.
Flow Cyt (Intra) 1/60.

Function

May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions.
Tissue specificity

Localized at tight junctions of both epithelial and endothelial cells. Highly expressed in kidney. Not detected in testis.
Involvement in disease

Defects in OCLN are the cause of band-like calcification with simplified gyration and polymicrogyria (BLCPMG) [MIM:251290]; also known as pseudo-TORCH syndrome. BLCPMG is a neurologic disorder with characteristic clinical and neuroradiologic features that mimic intrauterine TORCH infection in the absence of evidence of infection. Affected individuals have congenital microcephaly, intracranial calcifications, and severe developmental delay.
Sequence similarities

Belongs to the ELL/occludin family.
Contains 1 MARVEL domain.
Domain

The C-terminal is cytoplasmic and is important for interaction with ZO-1. Sufficient for the tight junction localization. Involved in the regulation of the permeability barrier function of the tight junction (By similarity). The first extracellular loop participates in an adhesive interaction.
Post-translational
modifications

Phosphorylated upon DNA damage, probably by ATM or ATR. Dephosphorylated by PTPRJ. The tyrosine phosphorylation on Tyr-398 and Tyr-402 reduces its ability to interact with TJP1.
Cellular localization

Membrane. Cell junction > tight junction.
Target information above from: UniProt accession Q16625 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 403844 Dog
- Entrez Gene: 100506658 Human
- Entrez Gene: 18260 Mouse
- Entrez Gene: 83497 Rat
- Omim: 602876 Human
- SwissProt: Q28269 Dog
- SwissProt: Q16625 Human
- SwissProt: Q61146 Mouse
- SwissProt: Q6P6T5 Rat
- Unigene: 592605 Human
- Unigene: 4807 Mouse
- Unigene: 31429 Rat
see all
Alternative names
- BLCPMG antibody
- FLJ08163 antibody
- FLJ18079 antibody
- FLJ77961 antibody
- FLJ94056 antibody
- MGC34277 antibody
- Occludin antibody
- Ocln antibody
- OCLN_HUMAN antibody
- Phosphatase 1 regulatory subunit 115 antibody
- PPP1R115 antibody
- PTORCH1 antibody
- Tight junction protein occludin antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody [EPR20992] (ab216327)

Tissue Microarrays stained for Anti-Occludin antibody [EPR20992] using ab216327 in immunohistochemical analysis. This table provides a detailed overview of positive (tick mark) and negaive (cross mark) staining per sample type tested. The sections were incubated with ab216327 at 4°C overnight used at 1:2000 dilution (1.05 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB secondary antibody (ab209101). Counterstain was Hematoxylin.

Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).
Western blot - Anti-Occludin antibody [EPR20992] (ab216327)

All lanes : Anti-Occludin antibody [EPR20992] (ab216327) at 1/1000 dilution

Lane 1 : Wild-type HAP1 whole cell lysate at 40 µg
Lane 2 : OCLN (Occludin) knockout HAP1 whole cell lysate at 40 µg
Lane 3 : HeLa whole cell lysate (Low Occludin expression) at 20 µg
Lane 4 : HepG2 whole cell lysate lysate (High Occludin expression) at 20 µg

Predicted band size: 59 kDa

Lanes 1 - 4: Merged signal (red and green). Green - ab216327 observed at 59 kDa. Red - loading control, ab9484, observed at 37 kDa.

ab216327 was shown to recognize Occludin in wild-type HAP1 cells as signal was lost at the expected MW in OCLN (Occludin) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and OCLN (Occludin) knockout samples were subjected to SDS-PAGE. ab216327 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

Occludin expression in HeLa is expected to be negative.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody [EPR20992] (ab216327)

Immunohistochemical analysis of paraffin-embedded human thyroid carcinoma labeling Occludin with ab216327 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880), ready to use. Counter stained with Hematoxylin. Antigen retrieval was heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

Secondary antibody only control: ab209101 (Rabbit specific IHC polymer detection kit HRP/DAB).

Positive staining on human thyroid carcinoma. The section was incubated with ab216327 at 4°C overnight.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody [EPR20992] (ab216327)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Occludin with ab216327 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous staining on human colon is observed (PMID: 24268521). Counter stained with Hematoxylin.

Secondary antibody only control: ab209101 (Rabbit specific IHC polymer detection kit HRP/DAB).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-Occludin antibody [EPR20992] (ab216327)

All lanes : Anti-Occludin antibody [EPR20992] (ab216327) at 1/1000 dilution

Lane 1 : Caco-2 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : bEnd.3 (Mouse brain endothelioma) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

Predicted band size: 59 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?

Exposure time: 60 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-Occludin antibody [EPR20992] (ab216327)

All lanes : Anti-Occludin antibody [EPR20992] (ab216327) at 1/1000 dilution

Lane 1 : Mouse colon tissue lysate
Lane 2 : Mouse brain tissue lysate
Lane 3 : Mouse lung tissue lysate
Lane 4 : Rat colon tissue lysate
Lane 5 : Rat brain tissue lysate
Lane 6 : Rat lung tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

Predicted band size: 59 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?

Exposure time: 100 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunocytochemistry/ Immunofluorescence - Anti-Occludin antibody [EPR20992] (ab216327)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Caco-2 (human colorectal adenocarcinoma cell line) cells labeling Occludin with ab216327 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membrane staining on Caco-2 cell line.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
Western blot - Anti-Occludin antibody [EPR20992] (ab216327)

Lane 1 : Anti-Occludin antibody [EPR8208] (ab167161) at 1/5000 dilution
Lane 2 : Anti-Occludin antibody (ab31721) at 1/1000 dilution
Lane 3 : Anti-Occludin antibody [EPR20992] (ab216327) at 1/5000 dilution

All lanes : Recombinant Human Occludin protein (ab114189)

Lysates/proteins at 0.025 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 59 kDa
Observed band size: 85 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer: 5% NFDM/TBST

Exposure time: 5.5 seconds
Western blot - Anti-Occludin antibody [EPR20992] (ab216327)

All lanes : Anti-Occludin antibody [EPR20992] (ab216327) at 1/1000 dilution

Lane 1 : Human colon lysate at 20 µg
Lane 2 : MDCK (canine kidney cell line) cell lysate at 20 µg
Lane 3 : PC-3 (human prostate adenocarcinoma cell line) cell lysate at 20 µg
Lane 4 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate at 20 µg
Lane 5 : Caco-2 (human colorectal adenocarcinoma cell line) cell lysate at 10 µg

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 59 kDa
Observed band size: 25,53,65 kDa why is the actual band size different from the predicted?

Exposure time : Lanes 1-4: 3 minutes; lane 5: 1 minute.

Blocking/Dilution buffer: 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID: 18647175, PMID: 19821483).
Western blot - Anti-Occludin antibody [EPR20992] (ab216327)

All lanes : Anti-Occludin antibody [EPR20992] (ab216327) at 1/1000 dilution

Lane 1 : Rat brain lysate
Lane 2 : Mouse brain lysate
Lane 3 : Mouse uterus lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 59 kDa
Observed band size: 23,53,65 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID: 18647175, PMID: 19821483).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody [EPR20992] (ab216327)

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Occludin with ab216327 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining on distal tubules of mouse kidney is observed. Counter stained with Hematoxylin.

Secondary antibody only control: ab209101 (Rabbit specific IHC polymer detection kit HRP/DAB).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody [EPR20992] (ab216327)

Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling Occludin with ab216327 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining on distal tubules of rat kidney is observed. Counter stained with Hematoxylin.

Secondary antibody only control: ab209101 (Rabbit specific IHC polymer detection kit HRP/DAB).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody [EPR20992] (ab216327)

Immunohistochemical analysis of paraffin-embedded human breast tissue labeling Occludin with ab216327 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous staining on human breast is observed. Counter stained with Hematoxylin.

Secondary antibody only control: ab209101 (Rabbit specific IHC polymer detection kit HRP/DAB).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-Occludin antibody [EPR20992] (ab216327)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MDCK (canine kidney cell line) cells labeling Occludin with ab216327 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membrane staining on MDCK (NBL-2) cell line.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
Immunoprecipitation - Anti-Occludin antibody [EPR20992] (ab216327)

Occludin was immunoprecipitated from 0.35 mg of Caco-2 (human colorectal adenocarcinoma cell line) whole cell lysate with ab216327 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab216327 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution

Lane 1: Caco-2 whole cell lysate 10 µg (Input).

Lane 2: ab216327 IP in Caco-2 whole cell lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab216327 in Caco-2 whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 1 second.

The molecular weight observed is consistent with what has been described in the literature (PMID: 18647175, PMID: 19821483).
Flow Cytometry (Intracellular) - Anti-Occludin antibody [EPR20992] (ab216327)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized Caco-2 (human colorectal adenocarcinoma cell line) cell line labeling Occludin with ab216327 at 1/60 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.
Anti-Occludin antibody [EPR20992] (ab216327)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

Publishing research using ab216327? Please let us know so that we can cite the reference in this datasheet.

ab216327 has been referenced in 165 publications.

Huang YM et al. Glucagon-Like Peptide-2 Ameliorates Age-Associated Bone Loss and Gut Barrier Dysfunction in Senescence-Accelerated Mouse Prone 6 Mice. Gerontology 69:428-449 (2023). PubMed: 36470214
Hill AA et al. Acute high-fat diet impairs macrophage-supported intestinal damage resolution. JCI Insight 8:N/A (2023). PubMed: 36538527
Xu P et al. Intestinal microbiota analysis and network pharmacology reveal the mechanism by which Lianhua Qingwen capsule improves the immune function of mice infected with influenza A virus. Front Microbiol 13:1035941 (2022). PubMed: 36504796
Harati R et al. miR-27a-3p regulates expression of intercellular junctions at the brain endothelium and controls the endothelial barrier permeability. PLoS One 17:e0262152 (2022). PubMed: 35025943
Liu N et al. Lactobacillus rhamnosus Ameliorates Multi-Drug-Resistant Bacillus cereus-Induced Cell Damage through Inhibition of NLRP3 Inflammasomes and Apoptosis in Bovine Endometritis. Microorganisms 10:N/A (2022). PubMed: 35056585

View all Publications for this product

Customer reviews and Q&As

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1-7 of 7 Abreviews or Q&A

Application

Western blot

Sample

Mouse Tissue lysate - whole (Mouse brain)

Gel Running Conditions

Reduced Denaturing (4-12% Bis-tris in MES)

Loading amount

10 µg

Specification

Mouse brain

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jun 15 2018

Application

Western blot

Sample

Mouse Tissue lysate - whole (Mouse brain)

Gel Running Conditions

Reduced Denaturing (4-12% Bis-tris in MOPS)

Loading amount

10 µg

Specification

Mouse brain

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Oct 27 2017

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Mouse Tissue sections (Brain)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0

Permeabilization

Specification

Brain

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Apr 11 2022

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (Brain)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0

Permeabilization

Specification

Brain

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Apr 11 2022

Application

Western blot

Sample

Pig Cell lysate - other (intestinal cell)

Gel Running Conditions

Reduced Denaturing (12%)

Loading amount

35 µg

Specification

intestinal cell

Blocking step

BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: RT°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Mar 11 2022

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Mouse Tissue sections (Intestine)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0

Permeabilization

Specification

Intestine

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Oct 13 2020

Application

Immunohistochemistry (Frozen sections)

Sample

Mouse Tissue sections (Testis, adult)

Permeabilization

Yes - 0.1% Triton X-100 in PBS

Specification

Testis, adult

Blocking step

BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Bryan Niedenberger

Verified customer

Submitted Apr 28 2019

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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Specificity

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Isotype control

Recombinant Protein

Related Products

Applications

The Abpromise guarantee

Target

Function

Tissue specificity

Involvement in disease

Sequence similarities

Domain

Post-translationalmodifications

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

Certificate of Compliance

References (165)

Customer reviews and Q&As

Western blot abreview for Anti-Occludin antibody [EPR20992]

Western blot abreview for Anti-Occludin antibody [EPR20992]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Occludin antibody [EPR20992]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Occludin antibody [EPR20992]

Western blot abreview for Anti-Occludin antibody [EPR20992]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Occludin antibody [EPR20992]

Immunohistochemistry (Frozen sections) abreview for Anti-Occludin antibody [EPR20992]

Post-translational
modifications