Recombinant Anti-Oct-1 antibody [EPR16570] (ab178869)
![Immunoprecipitation - Anti-Oct-1 antibody [EPR16570] (ab178869)](https://www.abcam.com/ps/products/178/ab178869/Images/ab178869-515859-anti-oct-1-antibody-epr16570-immunoprecipitation-human.jpg "Immunoprecipitation - Anti-Oct-1 antibody [EPR16570] (ab178869)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16570] to Oct-1
- Suitable for: WB, IHC-P, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
-
Product name
Anti-Oct-1 antibody [EPR16570]
See all Oct-1 primary antibodies -
Description
Rabbit monoclonal [EPR16570] to Oct-1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: Human fetal brain lysate; Mouse and Rat heart lysates; Ramos, HEK-293 and HeLa whole cell lysates. IHC-P: Human endometrium, Human tonsil, Human endometrial cancer, Mouse colon and Rat spleen tissues. IP: HeLa cell lysate.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16570 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab178869 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/1000. Detects a band of approximately 95 kDa (predicted molecular weight: 76 kDa).
|
| IHC-P | (3) |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| IP |
1/30.
ab178869 is suitable for [IP, Human]. ab178869 is not suitable for [IP, Mouse]. |
| Notes |
|---|
|
WB
1/1000. Detects a band of approximately 95 kDa (predicted molecular weight: 76 kDa). |
|
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
IP
1/30. ab178869 is suitable for [IP, Human]. ab178869 is not suitable for [IP, Mouse]. |
Target
-
Function
Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins. Modulates transcription transactivation by NR3C1, AR and PGR (By similarity). In case of human herpes simplex virus (HSV) infection, POU2F1 forms a multiprotein-DNA complex with the viral transactivator protein VP16 and HCFC1 thereby enabling the transcription of the viral immediate early genes. -
Tissue specificity
Ubiquitous. Isoform 2 is lymphocyte-specific. -
Sequence similarities
Belongs to the POU transcription factor family. Class-2 subfamily.
Contains 1 homeobox DNA-binding domain.
Contains 1 POU-specific domain. -
Post-translational
modificationsPhosphorylated by PRKDC. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 5451 Human
- Entrez Gene: 18986 Mouse
- Entrez Gene: 171068 Rat
- Omim: 164175 Human
- SwissProt: P14859 Human
- SwissProt: P25425 Mouse
- SwissProt: P31503 Rat
- Unigene: 283402 Human
see all -
Alternative names
- FLJ42836 antibody
- NF A1 antibody
- NF-A1 antibody
see all
Images
-
Immunoprecipitation - Anti-Oct-1 antibody [EPR16570] (ab178869)
Oct-1 was immunoprecipitated from 0.35mg HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate with ab178869 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab178869 at 1/1000 dilution (0.357 μg/ml). VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.
Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate (10ug).
Lane 2: ab178869 IP in HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab178869 in HeLa whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 24 seconds.
Observed band: 76 kDa.
Lysates were made freshly and used in IP test immediately to minimize protein degradation.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct-1 antibody [EPR16570] (ab178869)](https://www.abcam.com/ps/products/178/ab178869/Images/ab178869-252623-anti-oct-1-antibody-epr16570-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct-1 antibody [EPR16570] (ab178869)Immunohistochemical analysis of paraffin-embedded Human endometrium tissue labeling Oct-1 with ab178869 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining on Human endometrial tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct-1 antibody [EPR16570] (ab178869)](https://www.abcam.com/ps/products/178/ab178869/Images/ab178869-252622-anti-oct-1-antibody-epr16570-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct-1 antibody [EPR16570] (ab178869)Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling Oct-1 with ab178869 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining on Human tonsil tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct-1 antibody [EPR16570] (ab178869)](https://www.abcam.com/ps/products/178/ab178869/Images/ab178869-252620-anti-oct-1-antibody-epr16570-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct-1 antibody [EPR16570] (ab178869)Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling Oct-1 with ab178869 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining on epithelium of mouse colon is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct-1 antibody [EPR16570] (ab178869)](https://www.abcam.com/ps/products/178/ab178869/Images/ab178869-252621-anti-oct-1-antibody-epr16570-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct-1 antibody [EPR16570] (ab178869)Immunohistochemical analysis of paraffin-embedded Human endometrial cancer tissue labeling Oct-1 with ab178869 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining on tumor cells of Human endometrial cancer tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct-1 antibody [EPR16570] (ab178869)](https://www.abcam.com/ps/products/178/ab178869/Images/ab178869-252619-anti-oct-1-antibody-epr16570-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct-1 antibody [EPR16570] (ab178869)Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling Oct-1 with ab178869 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining on rat spleen tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Western blot - Anti-Oct-1 antibody [EPR16570] (ab178869)](https://www.abcam.com/ps/products/178/ab178869/Images/ab178869-252626-anti-oct-1-antibody-epr16570-western-blot.jpg)
Western blot - Anti-Oct-1 antibody [EPR16570] (ab178869)Anti-Oct-1 antibody [EPR16570] (ab178869) at 1/1000 dilution + Human fetal brain lysate at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 76 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteBlocking/Dilution buffer: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID: 9398664; 14586402; 11777930).
-
![Western blot - Anti-Oct-1 antibody [EPR16570] (ab178869)](https://www.abcam.com/ps/products/178/ab178869/Images/ab178869-252625-anti-oct-1-antibody-epr16570-western-blot.jpg)
Western blot - Anti-Oct-1 antibody [EPR16570] (ab178869)All lanes : Anti-Oct-1 antibody [EPR16570] (ab178869) at 1/5000 dilution
Lane 1 : Ramos (Human Burkitt's lymphoma cell line) whole cell lysate
Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 76 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?
Exposure time: 3 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID: 9398664; 14586402; 11777930).
-
![Western blot - Anti-Oct-1 antibody [EPR16570] (ab178869)](https://www.abcam.com/ps/products/178/ab178869/Images/ab178869-252624-anti-oct-1-antibody-epr16570-western-blot.jpg)
Western blot - Anti-Oct-1 antibody [EPR16570] (ab178869)All lanes : Anti-Oct-1 antibody [EPR16570] (ab178869) at 1/1000 dilution
Lane 1 : Mouse heart lysate
Lane 2 : Rat heart lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 76 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteBlocking/Dilution buffer: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID: 9398664; 14586402; 11777930).
Please note that the antibody detects extra bands on lysates from mouse and rat cell lines.
-
![Anti-Oct-1 antibody [EPR16570] (ab178869)](https://www.abcam.com/ps/products/178/ab178869/Images/ab178869-9-benefits-of-recombinant-antibodies.png)
Anti-Oct-1 antibody [EPR16570] (ab178869)
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
Certificate of Compliance
References (10)
ab178869 has been referenced in 10 publications.
- Bai X et al. Regulation of CYP450 and drug transporter mediated by gut microbiota under high-altitude hypoxia. Front Pharmacol 13:977370 (2022). PubMed: 36188572
- Wang Q et al. Mechanisms Underlying the Differences in the Pharmacokinetics of Six Active Constituents of Huangqi Liuyi Decoction between Normal and Diabetic Nephropathy Mouse Models. Evid Based Complement Alternat Med 2022:2481654 (2022). PubMed: 36285162
- Sun B et al. Regulation of host and virus genes by neuronal miR-138 favours herpes simplex virus 1 latency. Nat Microbiol 6:682-696 (2021). PubMed: 33558653
- Li Z et al. Octamer transcription factor-1 induces the Warburg effect via up-regulation of hexokinase 2 in non-small cell lung cancer. Mol Cell Biochem 476:3423-3431 (2021). PubMed: 33970409
- Li M et al. Pathological matrix stiffness promotes cardiac fibroblast differentiation through the POU2F1 signaling pathway. Sci China Life Sci 64:242-254 (2021). PubMed: 32617828
- Song H et al. BRD4 promotes the stemness of gastric cancer cells via attenuating miR-216a-3p-mediated inhibition of Wnt/ß-catenin signaling. Eur J Pharmacol 852:189-197 (2019). PubMed: 30876979
- Zhu Z et al. Vasopressin regulates hypothalamic GnRH synthesis: Histomorphological evidence in hypothalamus and biological effects in GT1-7 cells. Life Sci 227:166-174 (2019). PubMed: 31026452
- Vázquez-Arreguín K et al. Oct1/Pou2f1 is selectively required for colon regeneration and regulates colon malignancy. PLoS Genet 15:e1007687 (2019). PubMed: 31059499
- Vázquez-Arreguín K et al. BRCA1 through Its E3 Ligase Activity Regulates the Transcription Factor Oct1 and Carbohydrate Metabolism. Mol Cancer Res 16:439-452 (2018). PubMed: 29330289
- Song H et al. LncRNA THOR increases the stemness of gastric cancer cells via enhancing SOX9 mRNA stability. Biomed Pharmacother 108:338-346 (2018). PubMed: 30227327