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    products/primary-antibodies/oct-2-antibody-ep543y-ab33919.pdf

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Epigenetics and Nuclear Signaling Transcription Domain Families Developmental Families OCT
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RabMAb

Anti-Oct-2 antibody [EP543Y] (ab33919)

  • Datasheet
  • SDS
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Western blot - Anti-Oct-2 antibody [EP543Y] (ab33919)

    Key features and details

    • Rabbit monoclonal [EP543Y] to Oct-2
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    Conjugates logo Related conjugates and formulations

    Carrier Free

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    Overview

    • Product name

      Anti-Oct-2 antibody [EP543Y]
      See all Oct-2 primary antibodies
    • Description

      Rabbit monoclonal [EP543Y] to Oct-2
    • Host species

      Rabbit
    • Specificity

      This antibody may detect the isoform 2.

    • Tested applications

      Suitable for: WBmore details
      Unsuitable for: Flow Cyt
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

    • Positive control

      • Raji cell lysate
    • General notes

      Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

      Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.20
      Preservative: 0.01% Sodium azide
      Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
    • Concentration information loading...
    • Purity

      Protein A purified
    • Clonality

      Monoclonal
    • Clone number

      EP543Y
    • Isotype

      IgG
    • Research areas

      • Epigenetics and Nuclear Signaling
      • Transcription
      • Domain Families
      • Developmental Families
      • OCT

    Associated products

    • Isotype control

      • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab33919 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    1/20000 - 1/50000. Predicted molecular weight: 51 kDa.
    Notes
    WB
    1/20000 - 1/50000. Predicted molecular weight: 51 kDa.
    Application notes
    Is unsuitable for Flow Cyt.

    Target

    • Function

      Transcription factor that specifically binds to the octamer motif (5'-ATTTGCAT-3'). Regulates transcription in a number of tissues in addition to activating immunoglobulin gene expression. Modulates transcription transactivation by NR3C1, AR and PGR. Isoform 5 activates the U2 small nuclear RNA (snRNA) promoter.
    • Tissue specificity

      Isoform 3 is B-cell specific. Isoform 5 is expressed in B-cells and the immunoglobulin-expressing T-cell line MOLT-4, but not in the T-cell line BW5147.
    • Sequence similarities

      Belongs to the POU transcription factor family. Class-2 subfamily.
      Contains 1 homeobox DNA-binding domain.
      Contains 1 POU-specific domain.
    • Cellular localization

      Cytoplasm. Nucleus.
    • Target information above from: UniProt accession P09086 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 5452 Human
      • Omim: 164176 Human
      • SwissProt: P09086 Human
      • Unigene: 147381 Human
      • Alternative names

        • class 2 antibody
        • Lymphoid restricted immunoglobulin octamer binding protein antibody
        • Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2 antibody
        • NF A2 antibody
        • Oct 2 antibody
        • Oct-2 antibody
        • Octamer binding transcription factor 2 antibody
        • Octamer-binding protein 2 antibody
        • Octamer-binding transcription factor 2 antibody
        • OTF-2 antibody
        • OTF2 antibody
        • PO2F2_HUMAN antibody
        • POU domain antibody
        • POU domain class 2 transcription factor 2 antibody
        • POU2F2 antibody
        • transcription factor 2 antibody
        see all

      Images

      • Western blot - Anti-Oct-2 antibody [EP543Y] (ab33919)
        Western blot - Anti-Oct-2 antibody [EP543Y] (ab33919)
        Anti-Oct-2 antibody [EP543Y] (ab33919) at 1/50000 dilution + Raji cell lysate

        Predicted band size: 51 kDa
        Observed band size: 55 kDa why is the actual band size different from the predicted?

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (3)

      Publishing research using ab33919? Please let us know so that we can cite the reference in this datasheet.

      ab33919 has been referenced in 3 publications.

      • Wei L  et al. Functional role of PLCE1 intronic insertion variant associated with susceptibility to esophageal squamous cell carcinoma. Carcinogenesis 39:191-201 (2018). PubMed: 29106514
      • Oguri T  et al. Organic cation transporter 6 directly confers resistance to anticancer platinum drugs. Biomed Rep 5:639-643 (2016). WB ; Human . PubMed: 27882231
      • Winiarska M  et al. Prenyltransferases Regulate CD20 Protein Levels and Influence Anti-CD20 Monoclonal Antibody-mediated Activation of Complement-dependent Cytotoxicity. J Biol Chem 287:31983-93 (2012). ChIP . PubMed: 22843692

      Customer reviews and Q&As

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      There are currently no Customer reviews or Questions for ab33919.
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