Recombinant Anti-Oct-2 antibody [EPR12482-106] (ab178679)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct-2 antibody [EPR12482-106] (ab178679)](https://www.abcam.com/ps/products/178/ab178679/Images/ab178679-206860-anti-oct-2-antibody-epr12482-106-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct-2 antibody [EPR12482-106] (ab178679)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12482-106] to Oct-2
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Oct-2 antibody [EPR12482-106]
See all Oct-2 primary antibodies -
Description
Rabbit monoclonal [EPR12482-106] to Oct-2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Rat
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Immunogen
Recombinant fragment within Human Oct-2. The exact sequence is proprietary.
Database link: P09086 -
Positive control
- Ramos, Raji, Daudi and Jurkat whole cell lysate (ab7899). Human colon, tonsil and DLBCL tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12482-106 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab178679 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/5000. Detects a band of approximately 58 kDa (predicted molecular weight: 51 kDa).
|
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| IHC-P | (2) |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
WB
1/1000 - 1/5000. Detects a band of approximately 58 kDa (predicted molecular weight: 51 kDa). |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Transcription factor that specifically binds to the octamer motif (5'-ATTTGCAT-3'). Regulates transcription in a number of tissues in addition to activating immunoglobulin gene expression. Modulates transcription transactivation by NR3C1, AR and PGR. Isoform 5 activates the U2 small nuclear RNA (snRNA) promoter. -
Tissue specificity
Isoform 3 is B-cell specific. Isoform 5 is expressed in B-cells and the immunoglobulin-expressing T-cell line MOLT-4, but not in the T-cell line BW5147. -
Sequence similarities
Belongs to the POU transcription factor family. Class-2 subfamily.
Contains 1 homeobox DNA-binding domain.
Contains 1 POU-specific domain. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 5452 Human
- Entrez Gene: 117058 Rat
- Omim: 164176 Human
- SwissProt: P09086 Human
- Unigene: 147381 Human
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Alternative names
- class 2 antibody
- Lymphoid restricted immunoglobulin octamer binding protein antibody
- Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct-2 antibody [EPR12482-106] (ab178679)
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Oct-2 with ab178679 at 1:100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct-2 antibody [EPR12482-106] (ab178679)](https://www.abcam.com/ps/products/178/ab178679/Images/ab178679-206859-anti-oct-2-antibody-epr12482-106-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct-2 antibody [EPR12482-106] (ab178679)Immunohistochemical analysis of paraffin-embedded Human DLBCL tissue labeling Oct-2 with ab178679 at 1:100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct-2 antibody [EPR12482-106] (ab178679)](https://www.abcam.com/ps/products/178/ab178679/Images/ab178679-206858-anti-oct-2-antibody-epr12482-106-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct-2 antibody [EPR12482-106] (ab178679)Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling Oct-2 with ab178679 at 1:100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-Oct-2 antibody [EPR12482-106] (ab178679)](https://www.abcam.com/ps/products/178/ab178679/Images/ab178679-206861-anti-oct-2-antibody-epr12482-106-western-blot.jpg)
Western blot - Anti-Oct-2 antibody [EPR12482-106] (ab178679)All lanes : Anti-Oct-2 antibody [EPR12482-106] (ab178679) at 1/1000 dilution
Lane 1 : Ramos cell lysate
Lane 2 : Raji cell lysate
Lane 3 : Daudi cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 51 kDa -
![Anti-Oct-2 antibody [EPR12482-106] (ab178679)](https://www.abcam.com/ps/products/178/ab178679/Images/ab178679-5-benefits-of-recombinant-antibodies.png)
Anti-Oct-2 antibody [EPR12482-106] (ab178679)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab178679 has been referenced in 2 publications.
- Harris A et al. Onecut Factors and Pou2f2 Regulate the Distribution of V2 Interneurons in the Mouse Developing Spinal Cord. Front Cell Neurosci 13:184 (2019). PubMed: 31231191
- Masgutova G et al. Pou2f2 Regulates the Distribution of Dorsal Interneurons in the Mouse Developing Spinal Cord. Front Mol Neurosci 12:263 (2019). PubMed: 31787878