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RecombinantRabMAb

Recombinant Anti-Oct-2 antibody [EPR12482-106] - BSA and Azide free (ab250055)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct-2 antibody [EPR12482-106] - BSA and Azide free (ab250055)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct-2 antibody [EPR12482-106] - BSA and Azide free (ab250055)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct-2 antibody [EPR12482-106] - BSA and Azide free (ab250055)
  • Western blot - Anti-Oct-2 antibody [EPR12482-106] - BSA and Azide free (ab250055)
  • Anti-Oct-2 antibody [EPR12482-106] - BSA and Azide free (ab250055)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12482-106] to Oct-2 - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Unconjugated

Overview

  • Product name

    Anti-Oct-2 antibody [EPR12482-106] - BSA and Azide free
    See all Oct-2 primary antibodies

  • Description

    Rabbit monoclonal [EPR12482-106] to Oct-2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rat

  • Immunogen

    Recombinant fragment within Human Oct-2. The exact sequence is proprietary.
    Database link: P09086

  • General notes

    ab250055 is the carrier-free version of ab178679.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab250055 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use at an assay dependent concentration. Detects a band of approximately 58 kDa (predicted molecular weight: 51 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
Use at an assay dependent concentration. Detects a band of approximately 58 kDa (predicted molecular weight: 51 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Application notes
Is unsuitable for Flow Cyt,ICC/IF or IP.

Target

  • Function

    Transcription factor that specifically binds to the octamer motif (5'-ATTTGCAT-3'). Regulates transcription in a number of tissues in addition to activating immunoglobulin gene expression. Modulates transcription transactivation by NR3C1, AR and PGR. Isoform 5 activates the U2 small nuclear RNA (snRNA) promoter.

  • Tissue specificity

    Isoform 3 is B-cell specific. Isoform 5 is expressed in B-cells and the immunoglobulin-expressing T-cell line MOLT-4, but not in the T-cell line BW5147.

  • Sequence similarities

    Belongs to the POU transcription factor family. Class-2 subfamily.
    Contains 1 homeobox DNA-binding domain.
    Contains 1 POU-specific domain.

  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • class 2 antibody
      • Lymphoid restricted immunoglobulin octamer binding protein antibody
      • Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2 antibody
      • NF A2 antibody
      • Oct 2 antibody
      • Oct-2 antibody
      • Octamer binding transcription factor 2 antibody
      • Octamer-binding protein 2 antibody
      • Octamer-binding transcription factor 2 antibody
      • OTF-2 antibody
      • OTF2 antibody
      • PO2F2_HUMAN antibody
      • POU domain antibody
      • POU domain class 2 transcription factor 2 antibody
      • POU2F2 antibody
      • transcription factor 2 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct-2 antibody [EPR12482-106] - BSA and Azide free (ab250055)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct-2 antibody [EPR12482-106] - BSA and Azide free (ab250055)
      This data was developed using ab178679, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Oct-2 with ab178679 at 1:100 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct-2 antibody [EPR12482-106] - BSA and Azide free (ab250055)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct-2 antibody [EPR12482-106] - BSA and Azide free (ab250055)
      This data was developed using ab178679, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human DLBCL tissue labeling Oct-2 with ab178679 at 1:100 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct-2 antibody [EPR12482-106] - BSA and Azide free (ab250055)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct-2 antibody [EPR12482-106] - BSA and Azide free (ab250055)
      This data was developed using ab178679, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling Oct-2 with ab178679 at 1:100 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
    • Western blot - Anti-Oct-2 antibody [EPR12482-106] - BSA and Azide free (ab250055)
      Western blot - Anti-Oct-2 antibody [EPR12482-106] - BSA and Azide free (ab250055)
      All lanes : Anti-Oct-2 antibody [EPR12482-106] (ab178679) at 1/1000 dilution

      Lane 1 : Ramos cell lysate
      Lane 2 : Raji cell lysate
      Lane 3 : Daudi cell lysate
      Lane 4 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 51 kDa



      This data was developed using ab178679, the same antibody clone in a different buffer formulation.

       

    • Anti-Oct-2 antibody [EPR12482-106] - BSA and Azide free (ab250055)
      Anti-Oct-2 antibody [EPR12482-106] - BSA and Azide free (ab250055)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab250055? Please let us know so that we can cite the reference in this datasheet.

    ab250055 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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