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RecombinantRabMAb

Recombinant Anti-Oct-2 antibody [EPR542] - BSA and Azide free (ab239913)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct-2 antibody [EPR542] - BSA and Azide free (ab239913)
  • Immunocytochemistry/ Immunofluorescence - Anti-Oct-2 antibody [EPR542] - BSA and Azide free (ab239913)
  • Flow Cytometry (Intracellular) - Anti-Oct-2 antibody [EPR542] - BSA and Azide free (ab239913)
  • Anti-Oct-2 antibody [EPR542] - BSA and Azide free (ab239913)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR542] to Oct-2 - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), ICC/IF, IHC-P, WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alkaline Phosphatase APC HRP PE Unconjugated

Overview

  • Product name

    Anti-Oct-2 antibody [EPR542] - BSA and Azide free
    See all Oct-2 primary antibodies

  • Description

    Rabbit monoclonal [EPR542] to Oct-2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), ICC/IF, IHC-P, WBmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: Raji cells

  • General notes

    ab239913 is the carrier-free version of ab92458.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab239913 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration. Detects a band of approximately 58 kDa (predicted molecular weight: 51 kDa).
Notes
Flow Cyt (Intra)
Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration. Detects a band of approximately 58 kDa (predicted molecular weight: 51 kDa).
Application notes
Is unsuitable for IP.

Target

  • Function

    Transcription factor that specifically binds to the octamer motif (5'-ATTTGCAT-3'). Regulates transcription in a number of tissues in addition to activating immunoglobulin gene expression. Modulates transcription transactivation by NR3C1, AR and PGR. Isoform 5 activates the U2 small nuclear RNA (snRNA) promoter.

  • Tissue specificity

    Isoform 3 is B-cell specific. Isoform 5 is expressed in B-cells and the immunoglobulin-expressing T-cell line MOLT-4, but not in the T-cell line BW5147.

  • Sequence similarities

    Belongs to the POU transcription factor family. Class-2 subfamily.
    Contains 1 homeobox DNA-binding domain.
    Contains 1 POU-specific domain.

  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • class 2 antibody
      • Lymphoid restricted immunoglobulin octamer binding protein antibody
      • Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2 antibody
      • NF A2 antibody
      • Oct 2 antibody
      • Oct-2 antibody
      • Octamer binding transcription factor 2 antibody
      • Octamer-binding protein 2 antibody
      • Octamer-binding transcription factor 2 antibody
      • OTF-2 antibody
      • OTF2 antibody
      • PO2F2_HUMAN antibody
      • POU domain antibody
      • POU domain class 2 transcription factor 2 antibody
      • POU2F2 antibody
      • transcription factor 2 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct-2 antibody [EPR542] - BSA and Azide free (ab239913)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct-2 antibody [EPR542] - BSA and Azide free (ab239913)

      ab92458, at 1:250 staining Oct-2 in Human tonsil paraffin embeeded section by immunohistochemistry.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92458).

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-Oct-2 antibody [EPR542] - BSA and Azide free (ab239913)
      Immunocytochemistry/ Immunofluorescence - Anti-Oct-2 antibody [EPR542] - BSA and Azide free (ab239913)
      This data was developed using the same antibody clone in a different buffer formulation (ab92458).
      Immunocytochemistry analysis of Raji (human Burkitt's lymphoma B lymphocyte) labeling 37530 with purified ab92458 at 1/500 dilution. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.19 µg/ml) was used as counterstain. Nuclei were stained blue with DAPI.
      Negative control: PBS instead of the primary antibody.
    • Flow Cytometry (Intracellular) - Anti-Oct-2 antibody [EPR542] - BSA and Azide free (ab239913)
      Flow Cytometry (Intracellular) - Anti-Oct-2 antibody [EPR542] - BSA and Azide free (ab239913)

      Overlay histogram showing Raji cells stained with ab92458 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92458, 1/50) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. 

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92458). 

       

       

    • Anti-Oct-2 antibody [EPR542] - BSA and Azide free (ab239913)
      Anti-Oct-2 antibody [EPR542] - BSA and Azide free (ab239913)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab239913? Please let us know so that we can cite the reference in this datasheet.

    ab239913 has not yet been referenced specifically in any publications.

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