Name: Anti-Oct4 antibody [EPR2054]
Brand: RabMAb
SKU: ab109183
Rating: 5 (1 reviews)

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ChIC/CUT&RUN sequencing - Anti-Oct4 antibody [EPR2054] (ab109183)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR2054] to Oct4
Suitable for: ChIC/CUT&RUN-seq, WB, IP, IHC-P
Reacts with: Human

Carrier Free HRP

Product name

Anti-Oct4 antibody [EPR2054]
See all Oct4 primary antibodies
Description

Rabbit monoclonal [EPR2054] to Oct4
Host species

Rabbit
Tested applications

Suitable for: ChIC/CUT&RUN-seq, WB, IP, IHC-Pmore details
Unsuitable for: ICC/IF
Species reactivity

Reacts with: Human
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: NCCIT and HeLa whole cell lysate (ab150035). IHC-P: Human seminoma and ovarian dysgerminoma tissue. IP: NCCIT whole cell lysate. ChIC/CUT&RUN-Seq: NCCIT cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Dissociation constant (K_D)

K_D = 5.00 x 10 ^-12 M
Learn more about K_D
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR2054
Isotype

IgG
Research areas

Alternative Versions
- HRP Anti-Oct4 antibody [EPR2054] (ab194076)
- Anti-Oct4 antibody [EPR2054] - BSA and Azide free (ab222233)
Assay kits
- Oct4 Transcription Factor Assay Kit (Colorimetric) (ab207224)
Compatible Secondaries
Conjugation kits
- PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
- APC Conjugation Kit - Lightning-Link® (ab201807)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Recombinant Protein
- Recombinant human Oct4 protein (ab169842)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab109183 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
ChIC/CUT&RUN-seq		Use at an assay dependent concentration. 5 µg
WB	(1)	1/1000 - 1/10000. Predicted molecular weight: 39 kDa.
IP		1/10 - 1/100.
IHC-P		1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. See IHC antigen retrieval protocols.

Notes
ChIC/CUT&RUN-seq Use at an assay dependent concentration. 5 µg
WB 1/1000 - 1/10000. Predicted molecular weight: 39 kDa.
IP 1/10 - 1/100.
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. See IHC antigen retrieval protocols.

Application notes

Is unsuitable for ICC/IF.

Function

Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.
Tissue specificity

Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.
Sequence similarities

Belongs to the POU transcription factor family. Class-5 subfamily.
Contains 1 homeobox DNA-binding domain.
Contains 1 POU-specific domain.
Developmental stage

Highly expressed in undifferentiated embryonic stem cells and expression decreases gradually after embryoid body (EB) formation.
Domain

The POU-specific domain mediates interaction with PKM2.
Post-translational
modifications

Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression.
Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation.
Cellular localization

Nucleus. Expressed in a diffuse and slightly punctuate pattern.
Target information above from: UniProt accession Q01860 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 5460 Human
- Omim: 164177 Human
- SwissProt: Q01860 Human
- Unigene: 249184 Human
- Unigene: 632482 Human
- Unigene: 646545 Human
Alternative names
- Octamer binding transcription factor 4 antibody
- MGC22487 antibody
- Oct 3 antibody
- Oct 4 antibody
- Oct-3 antibody
- Oct-4 antibody
- OCT3 antibody
- Oct4 antibody
- Octamer binding protein 3 antibody
- Octamer binding protein 4 antibody
- Octamer binding transcription factor 3 antibody
- Octamer-binding protein 3 antibody
- Octamer-binding protein 4 antibody
- Octamer-binding transcription factor 3 antibody
- OTF 3 antibody
- OTF 4 antibody
- OTF-3 antibody
- OTF3 antibody
- OTF4 antibody
- PO5F1_HUMAN antibody
- POU class 5 homeobox 1 antibody
- POU domain class 5 transcription factor 1 antibody
- POU domain transcription factor OCT4 antibody
- POU domain, class 5, transcription factor 1 antibody
- POU-type homeodomain-containing DNA-binding protein antibody
- POU5F1 antibody
see all

ChIC/CUT&RUN sequencing - Anti-Oct4 antibody [EPR2054] (ab109183)

ChIC/CUT&RUN was performed using a pAG-MNAse at a final concentration of 700 ng/µL, 2 x 10^5 NCCIT (Human pluripotent embryonic carcinoma cell line) cells and 5 µg of ab109183 [EPR2054]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown.

Additional screenshots of mapped reads can be downloaded here.

The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.
Western blot - Anti-Oct4 antibody [EPR2054] (ab109183)

Anti-Oct4 antibody [EPR2054] (ab109183) at 0.06 µg/ml (purified) + NCCIT (Human pluripotent embryonic carcinoma epithelial cell) whole cell lysates at 15 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 39 kDa

Blocking and diluting buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct4 antibody [EPR2054] (ab109183)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human seminoma tissue sections labeling Oct4 - with Purified ab109183 at 1:600 dilution (1 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylinwas used as a counterstain
Western blot - Anti-Oct4 antibody [EPR2054] (ab109183)

All lanes : Anti-Oct4 antibody [EPR2054] (ab109183) at 1/1000 dilution (Unpurified)

Lane 1 : Seminoma lysate
Lane 2 : NCCIT cell lysate
Lane 3 : HeLa cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 39 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct4 antibody [EPR2054] (ab109183)

Unpurified ab109183, at 1:500 dilution, staining Oct4 in paraffin-embedded Human ovarian dysgerminoma by Immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunoprecipitation - Anti-Oct4 antibody [EPR2054] (ab109183)

Unpurified ab109183 at 1/50 immunoprecipitating Oct4 in NCCIT (human pluripotent embryonal carcinoma) whole cell lysate observed at 39 KDa (lanes 1 and 2).

Lane 1 (input): NCCIT whole cell lysate, 10μg

Lane 2 (+): ab109183 + NCCIT whole cell lysate

Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab109183 in NCCIT whole cell lysate

For western blotting, ab109183 at 1/1000 dilution and VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct4 antibody [EPR2054] (ab109183)

Unpurified ab109183 showing negative staining in Colonic adenocarcinoma tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct4 antibody [EPR2054] (ab109183)

Unpurified ab109183 showing negative staining in Skeletal muscle tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct4 antibody [EPR2054] (ab109183)

Unpurified ab109183 showing negative staining in Normal liver tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct4 antibody [EPR2054] (ab109183)

Unpurified ab109183 showing positive staining in Seminoma tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct4 antibody [EPR2054] (ab109183)

Unpurified ab109183 showing negative staining in Normal brain tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct4 antibody [EPR2054] (ab109183)

Unpurified ab109183 showing positive staining in Fetal brain tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct4 antibody [EPR2054] (ab109183)

Unpurified ab109183 showing negative staining in Hepatocellular carcinoma tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct4 antibody [EPR2054] (ab109183)

Unpurified ab109183 showing positive staining in Embryonal carcinoma tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
OI-RD Scanning - Anti-Oct4 antibody [EPR2054] (ab109183)

Equilibrium disassociation constant (K_D)
Learn more about K_D

Click here to learn more about K_D
Anti-Oct4 antibody [EPR2054] (ab109183)

Flow cytometry protocols
Immunoprecipitation protocols
Immunohistochemistry protocols
Western blot protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

Publishing research using ab109183? Please let us know so that we can cite the reference in this datasheet.

ab109183 has been referenced in 39 publications.

Shen P et al. LncRNA CASC15 regulates breast cancer cell stemness via the miR-654-5p/MEF2D axis. J Biochem Mol Toxicol 36:e23023 (2022). PubMed: 35235236
Salah M et al. Reactive oxygen species-inducing titanium peroxide nanoparticles as promising radiosensitizers for eliminating pancreatic cancer stem cells. J Exp Clin Cancer Res 41:146 (2022). PubMed: 35428310
Li H et al. Chelerythrine Chloride Inhibits Stemness of Melanoma Cancer Stem-Like Cells (CSCs) Potentially via Inducing Reactive Oxygen Species and Causing Mitochondria Dysfunction. Comput Math Methods Med 2022:4000733 (2022). PubMed: 35761835
Kaewpiboon C et al. Formoxanthone C Inhibits Malignant Tumor Phenotypes of Human A549 Multidrug Resistant-cancer Cells through Signal Transducer and Activator of Transcription 1-Histone Deacetylase 4 Signaling. J Cancer Prev 27:112-121 (2022). PubMed: 35864853
Li L et al. Knockdown of miR-92a suppresses the stemness of colorectal cancer cells via mediating SOCS3. Bioengineered 13:5613-5624 (2022). WB ; Human . PubMed: 35184640

View all Publications for this product

Customer reviews and Q&As

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1-6 of 6 Abreviews or Q&A

Application

Western blot

Sample

Human Purified protein (brain cancer)

Gel Running Conditions

Reduced Denaturing

Loading amount

20 µg

Specification

brain cancer

Blocking step

Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 26°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Apr 24 2015

Question

Dear Sir / Madam,
One of the biggest customer in stem cell lab would like to have an
antibody that is specific against Oct 4, either variant A or variant B
to distinguish between them. In literature, oct 4 antibodies are not
able to bind to specific variants.
What do you recommend?
Many Thanks and Best Regards,

Abcam community

Verified customer

Asked on Jul 13 2012

Answer

Thank you for your enquiry and your interest.

I can confirm that two isoforms of Oct 4 have been identified, and the exact amino acid sequences (aa) can be found at this website: http://www.uniprot.org/uniprot/Q01860

- Isoform A: Q01860-1 (1-360 aa)

- Isoform B: Q01860-2 (1-265 aa)

ab109183 - this antibody was raised against a peptide corresponding to the region between 265-360 aa, soit is very likely to recognize Isoform A.

ab18976 - the immunogen selected was around region 1-14 aa, thus it would recognize potentially both Isofm A and B.

If you need any further assistance in the future, please do not hesitate to contact me.

Abcam Scientific Support

Answered on Jul 13 2012

Question

Hola ,
¿Qué tal? estoy interesada en inmunofluorescencia
de doble marcaje en tejidos humanos parafinados.
En concreto me interesarían los anticuerpos primarios, a ser posible
monoclonales, de distintas especies para poder combinarlos, contra las
siguientes proteínas humanas:
* OCT4: ab18976, ab109183, ab129030
* tenascin C: ab6346, ab3970, ab108930, ab86182
* CD68: ab955, ab845, ab125047
* S100A6: ab11181, ab117714, ab75676
* ganglioside GD2: ab68456
* Sox10: ab107532, ab122977
* MMP9: ab76003, ab119906
* CD163: ab111250, ab100909
* PNMT: ab119784
He indicado los productos que me parecían interesantes de la web, pero
acepto sugerencias.
Y tambíen estaría interesada en los anticuerpos secundarios marcados
con distintos fluorocromos para anticuerpos de distintas especies.
Necesitaría la ficha técnica y el precio de cada uno de ellos.
Muchas gracias.
Saludos,

Abcam community

Verified customer

Asked on May 09 2012

Answer

Muchas gracias por tu respuesta.

Es importante que todos los anticuerpos que escojas para llevar a cabo IHC-P en tejido humano estén validados (y por tanto garantizados por Abcam) para funcionar en esta especie y aplicación. Esta información aparece en la hoja técnica de los productos.

También es importante echar un vistazo a las hojas de datos de cada uno de los productos para tener en cuenta recomendaciones especificas, tales como diluciones, métodos de recuperación antigénica, y demás que pueden aparecer en ellas. A las fichas técnicas se puede acceder desde la página web, escribiendo el código del anticuerpo (ab955, por ejemplo) en la casilla azul en la parte de arriba de nuestra página web (https://www.abcam.com/).

Desconozco las combinaciones que queréis llevar a cabo mediante inmunofluorescencia doble con los anticuerpos mencionados. En general, a la hora de hacer inmunoensayos múltiples hay que tener mucha precaución para evitar reactividad cruzada. Es importante usar anticuerpos procedentes de distintas especies si se puede, o al menos con diferentes isotipos. Una buena forma de evitar reactividad cruzada es usando secundarios de la misma especie. Además esto permite usar suero de dicha especie para bloquear.

Te copio los links a los protocolos que tenemos en nuestra web para llevar a cabo doble inmunofluorescencia por s pudieran resultar de ayuda:

https://www.abcam.com/index.html?pageconfig=resource&rid=11459

https://www.abcam.com/index.html?pageconfig=resource&rid=11458

De todas maneras si quieres que comentemos distintas posibilidades y escenarios posibles, no dudes en contactarme otra vez.

Respecto a los secundarios, tenemos muchos anticuerpos que podrían serviros. Te aconsejo que en función de los primarios y combinaciones que vayáis a llevar a cabo, optéis por uno u otro. Para llevar a cabo la búsqueda de los secundarios desde la pagina web (https://www.abcam.com/) pincha en la pestaña “Secondary Antibodies”. Se abre una nueva ventana de “Advance Search”, en la cual se pueden elegir las categorías requeridas. La única categoría obligatoria es el isotipo del anticuerpo, pues tiene que coincidir con el del primario. El resto de categorías son opcionales, pero son muy útiles para afinar la búsqueda y elegir el anticuerpo más apropiado según la especie del primario, la conjugación deseada, la clonalidad, la aplicación en la que se va a testar, etc…

Los precios y la disponibilidad pueden consultarse igualmente en la parte superior de la datasheet de los productos. Si necesitas un presupuesto no dudes en hacérmelo saber para que te lo envíe. Además tenemos algún descuento para pedidos superiores a 5 productos, por lo que si vais a realizar un pedido de estas características te animaría a que nos contactaras primero.

Espero que esta información sea de ayuda. Como te digo, para cualquier aclaración o más sugerencias, estaré encantada de echar una mano.

Abcam Scientific Support

Answered on May 09 2012

Question

I am a dealer in China, one of Our customer want purchase a Oct4 antibody：ab74538，they wanna know the product date of this antibody. It will be very helpful if you can tell me these information. Yours Ge Hao At 2012-01-06 05:07:05,technical@abcam.com wrote: body { margin: 0px; margin-top: 10px; font-family: arial, verdana, tahoma; background-color: #F5F5EE; p{ padding-top: 4px; margin: 0px; line-height: 18px; body table{ font-size: 12px; line-height: 100%; a { text-decoration: underline; color: #004a91; a:visited { color: #0096db; a:hover { color: #0096db; text-decoration: underline; .mainText { margin-left: 0cm; font-weight: normal; margin-left: 10px; .tagLine { margin-left: 0cm; font-weight: normal; margin-left: 10px; color: #FFFFFF; .tagLine a { margin-left: 0cm; font-weight: normal; text-decoration: none; color: #FFFFFF; .tagLink { margin-left: 0cm; font-weight: normal; text-decoration: none; color: #FFFFFF; font-weight: bold; .salutation{ margin-left: 0cm; padding-bottom: 10px; font-weight: bold; margin-left: 10px; https://www.abcam.com/index.html?utm_campaign=CRM&utm_source=Abcam.CRM&utm_medium=Email&utm_term=Header Dear Xinjiang We have an answer to your inquiry: Thank you for contacting Abcam regarding ab109183. I am sorry to hear that you have been experiencing difficulties with this antibody. Can you please provide some additional details as to the nature of the problems you are experiencing and your protocol? I am happy to help you in any way that I can. I look forward to your reply so that I may assist you further. Please do not hesitate to contact us if you have any additional questions. Help us improve our service. https://www.abcam.com/index.html?pageConfig=technicalSurvey&intCCEID=3402261 Your original inquiry to Abcam: hello,Dear abcam company, hello! I have a production of antibodies when the problem is anti-Oct4antibody [EPR2054] (ab109183), hope to get answers, thank you very much! yours Good: smooth, all is well! Thanks and greetings! MR. Ge Hao Customer Sales Representative Sales and technical baoxin (Xinjiang) Co., Ltd. Sales Urumqi, Xinjiang Medical University, Room 620, 6th Floor （830054） Tel： +86 (0991)4311457 FAX： +86 (0991)4312752 Ishikawa City University School of Medicine 32 Shihezi sub-district 2-151 （832003） Mobile：15999299363 Tel： +86 (0993)6668003 FAX: +86 (0993)6668003 E-mail：mailto:rnabio@163.com Best regards, Carolyn Carolyn Miazga, PhD Scientific Support Supervisor Abcam Inc. www.abcam.com Abcam Customer Services and Scientific Support Team https://www.abcam.com/index.html?pageconfig=technical&utm_campaign=CRM&utm_source=Abcam.CRM&utm_medium=Email&utm_term=Body [CCE3402261] Discover more at abcam.com

Abcam community

Verified customer

Asked on Jan 09 2012

Answer

Thank you for contacting Abcam. Regarding ab74538, when stored properly at -20, the antibody is expected to be stable for up to 5 years from receipt. I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions

Abcam Scientific Support

Answered on Jan 09 2012

Question

hello,Dear abcam company, hello! I have a production of antibodies when the problem is anti-Oct4antibody [EPR2054] (ab109183), hope to get answers, thank you very much!

Abcam community

Verified customer

Asked on Jan 05 2012

Answer

Thank you for contacting Abcam regarding ab109183. I am sorry to hear that you have been experiencing difficulties with this antibody. Can you please provide some additional details as to the nature of the problems you are experiencing and your protocol? I am happy to help you in any way that I can. I look forward to your reply so that I may assist you further. Please do not hesitate to contact us if you have any additional questions.

Abcam Scientific Support

Answered on Jan 05 2012

Question

Which is the isotype of this antibody? In tha datasheet is reported to be IgG1 but this is a Rabbit antibody, not a mouse. Which secondary antibody do you suggest to use for WB analysis?

Abcam community

Verified customer

Asked on Dec 20 2011

Answer

Thank you for contacting Abcam about this. I have changed the datasheet to remedy the error that you have noticed. Ab109183 Anti-Oct4 antibody [EPR2054] is a rabbit IgG isotype. When using in Western Blot I would recommend using one of the following secondary antibodies: ab97051: Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Rabbit-IgG-H-L-HRP-ab97051.html or ab97064: Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) https://www.abcam.com/Donkey-polyclonal-Secondary-Antibody-to-Rabbit-IgG-H-L-HRP-ab97064.html These products are covered by our Abpromise guarantee. At Abcam, providing quality service is just as important as providing quality products. Our Abpromise® ensures that you can trust our products to perform as stated on the datasheets, or we will offer a replacement, credit, or refund. The Abcam Abpromise® guarantee: • 100% Scientific and Customer Support for any product you buy from Abcam or one of our authorized distributors. • We guarantee our products work in the tested species and applications stated on the datasheet. • We will replace or refund products not performing as stated on the datasheet if reported within 6 months of purchase. • We investigate any quality concerns raised by customers to ensure our catalog contains products that perform to the highest standards. Please note these conditions to our Abpromise®: • Protocol information must be provided in order for the claim to be validated. • Antibodies tested in recombinant samples only are not guaranteed for use on endogenous samples. • “Predicted to react” information on the datasheets is provided for reference only and these species are not guaranteed. • Fast Track antibodies are covered for ELISA against the immunizing peptide only. I hope this information is helpful, but please do not hesitate to contact us with further questions.

Abcam Scientific Support

Answered on Dec 20 2011

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Dissociation constant (KD)

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Assay kits

Compatible Secondaries

Conjugation kits

Isotype control

Recombinant Protein

Applications

The Abpromise guarantee

Target

Function

Tissue specificity

Sequence similarities

Developmental stage

Domain

Post-translationalmodifications

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

References (39)

Customer reviews and Q&As

Western blot abreview for Anti-Oct4 antibody [EPR2054]

Dissociation constant (K_D)

Post-translational
modifications