Anti-OPA1 antibody [1E81D9] (ab119685)
![Flow Cytometry - Anti-OPA1 antibody [1E81D9] (ab119685)](https://www.abcam.com/ps/products/119/ab119685/Images/ab119685-204494-anti-opa1-antibody-1e81d9-flow-cytometry.jpg "Flow Cytometry - Anti-OPA1 antibody [1E81D9] (ab119685)")
Key features and details
- Mouse monoclonal [1E81D9] to OPA1
- Suitable for: WB, Flow Cyt
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Overview
-
Product name
Anti-OPA1 antibody [1E81D9]
See all OPA1 primary antibodies -
Description
Mouse monoclonal [1E81D9] to OPA1 -
Host species
Mouse -
Tested applications
Suitable for: WB, Flow Cytmore details
Unsuitable for: ELISA or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is considered to be commercially sensitive.
-
General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: 99% HEPES buffered saline -
Concentration information loading... -
Purity
Ammonium Sulphate Precipitation -
Purification notes
Purity is near homogeneity as judged by SDS-PAGE. ab119685 was produced in vitro using hybridomas grown in serum-free medium, and then concentrated by ammonium sulfate precipitation. -
Clonality
Monoclonal -
Clone number
1E81D9 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab119685 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
Use a concentration of 1 µg/ml. Predicted molecular weight: 112 kDa.
|
| Flow Cyt |
Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 112 kDa. |
|
Flow Cyt
Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Application notes
Is unsuitable for ELISA or IP.
Target
-
Function
Dynamin-related GTPase required for mitochondrial fusion and regulation of apoptosis. May form a diffusion barrier for proteins stored in mitochondrial cristae. Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space. -
Tissue specificity
Highly expressed in retina. Also expressed in brain, testis, heart and skeletal muscle. Isoform 1 expressed in retina, skeletal muscle, heart, lung, ovary, colon, thyroid gland, leukocytes and fetal brain. Isoform 2 expressed in colon, liver, kidney, thyroid gland and leukocytes. Low levels of all isoforms expressed in a variety of tissues. -
Involvement in disease
Defects in OPA1 are a cause of optic atrophy type 1 (OPA1) [MIM:165500]. OPA1 is a dominantly inherited optic neuropathy occurring in 1 in 50,000 individuals that features progressive loss in visual acuity leading, in many cases, to legal blindness.
Defects in OPA1 are the cause of optic atrophy 1 with deafness (OPA1D) [MIM:125250]. Some individuals with mutations in OPA1 manifest also ophthalmoplegia and myopathy. -
Sequence similarities
Belongs to the dynamin family. -
Post-translational
modificationsPARL-dependent proteolytic processing releases an antiapoptotic soluble form not required for mitochondrial fusion. -
Cellular localization
Mitochondrion inner membrane. Mitochondrion intermembrane space. - Information by UniProt
-
Database links
- Entrez Gene: 4976 Human
- Entrez Gene: 74143 Mouse
- Entrez Gene: 171116 Rat
- Omim: 605290 Human
- SwissProt: O60313 Human
- SwissProt: P58281 Mouse
- SwissProt: Q2TA68 Rat
- Unigene: 594504 Human
see all -
Alternative names
- Dynamin like 120 kDa protein antibody
- Dynamin like 120 kDa protein, mitochondrial antibody
- Dynamin-like 120 kDa protein, form S1 antibody
see all
Images
-
Flow Cytometry - Anti-OPA1 antibody [1E81D9] (ab119685)Overlay histogram showing SH-SY5Y cells stained with ab119685 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab119685, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
-
![Western blot - Anti-OPA1 antibody [1E81D9] (ab119685)](https://www.abcam.com/ps/products/119/ab119685/Images/ab119685-132834-anti-opa1-antibody-1e81d9-western-blot.jpg)
Western blot - Anti-OPA1 antibody [1E81D9] (ab119685)All lanes : Anti-OPA1 antibody [1E81D9] (ab119685) at 1 µg/ml
Lane 1 : whole cell lysates from HeLa cells(human)
Lane 2 : whole cell lysates from H4IIE cells(rat)
Lane 3 : whole cell lysates from MEF cells(mouse)
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP goat anti-mouse at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 112 kDa
Datasheets and documents
-
Datasheet download
References (8)
ab119685 has been referenced in 8 publications.
- Kochi C et al. Simulated vehicle exhaust exposure (SVEE) in rats impairs renal mitochondrial function. Clin Exp Hypertens 42:571-579 (2020). PubMed: 32423257
- Santos-Alves E et al. Physical exercise positively modulates DOX-induced hepatic oxidative stress, mitochondrial dysfunction and quality control signaling. Mitochondrion 47:103-113 (2019). PubMed: 31170523
- Yen JH et al. Activation of dynamin-related protein 1 - dependent mitochondria fragmentation and suppression of osteosarcoma by cryptotanshinone. J Exp Clin Cancer Res 38:42 (2019). PubMed: 30691497
- Bartsakoulia M et al. A novel mechanism causing imbalance of mitochondrial fusion and fission in human myopathies. Hum Mol Genet 27:1186-1195 (2018). PubMed: 29361167
- Takagi S et al. Ipragliflozin improves mitochondrial abnormalities in renal tubules induced by a high-fat diet. J Diabetes Investig N/A:N/A (2018). WB ; Mouse . PubMed: 29352520
- Rizo-Roca D et al. Modulation of mitochondrial biomarkers by intermittent hypobaric hypoxia and aerobic exercise after eccentric exercise in trained rats. Appl Physiol Nutr Metab 42:683-693 (2017). PubMed: 28177702
- Vishnyakova PA et al. Alterations in antioxidant system, mitochondrial biogenesis and autophagy in preeclamptic myometrium. BBA Clin 8:35-42 (2017). PubMed: 28736722
- Pfeffer G et al. Mutations in the SPG7 gene cause chronic progressive external ophthalmoplegia through disordered mitochondrial DNA maintenance. Brain 137:1323-36 (2014). PubMed: 24727571
