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RecombinantRabMAb

Recombinant Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] (ab203859)

Reviews (1) Submit a question References (6)
Western blot - Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] (ab203859)
  • Immunohistochemistry (Frozen sections) - Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] (ab203859)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] (ab203859)
  • Western blot - Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] (ab203859)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] (ab203859)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] (ab203859)
  • Immunohistochemistry (Frozen sections) - Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] (ab203859)
  • Immunoprecipitation - Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] (ab203859)
  • Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] (ab203859)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19725] to Ornithine Carbamoyltransferase/OTC
  • Suitable for: WB, IHC-P, IHC-Fr, IP
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725]
    See all Ornithine Carbamoyltransferase/OTC primary antibodies

  • Description

    Rabbit monoclonal [EPR19725] to Ornithine Carbamoyltransferase/OTC

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, IHC-Fr, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human fetal liver and small intestine lysates; Mouse and rat liver lysates. IHC-P: Human liver and liver cancer tissues. IHC-Fr: Mouse liver tissue. IP: Human fetal liver lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab203859 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).
IHC-P (1)
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P is recommended for human only.
IHC-Fr
1/500.

Antigen retrieval: Heated citrate solution (10mM citrate PH 6.0 + 0.05% Tween-20).
IP
1/40.
Notes
WB
1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P is recommended for human only.
IHC-Fr
1/500.

Antigen retrieval: Heated citrate solution (10mM citrate PH 6.0 + 0.05% Tween-20).
IP
1/40.

Target

  • Tissue specificity

    Mainly expressed in liver and intestinal mucosa.

  • Pathway

    Nitrogen metabolism; urea cycle; L-citrulline from L-ornithine and carbamoyl phosphate: step 1/1.

  • Involvement in disease

    Ornithine carbamoyltransferase deficiency

  • Sequence similarities

    Belongs to the ATCase/OTCase family.

  • Post-translational
    modifications

    Acetylation at Lys-88 negatively regulates ornithine carbamoyltransferase activity in response to nutrient signals.

  • Cellular localization

    Mitochondrion matrix.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • EC 2.1.3.3 antibody
    • MGC129967 antibody
    • MGC129968 antibody
    • MGC138856 antibody
    • OCTD antibody
    • Ornithine Carbamoyltransferase antibody
    • Ornithine carbamoyltransferase mitochondrial antibody
    • Ornithine carbamoyltransferase, mitochondrial antibody
    • Ornithine transcarbamylase antibody
    • Otc antibody
    • OTC_HUMAN antibody
    • OTCase antibody
    see all

Images

  • Western blot - Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] (ab203859)
    Western blot - Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] (ab203859)
    Lane 1 : Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] (ab203859) at 1/5000 dilution
    Lane 2 : Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] (ab203859) at 1/1000 dilution

    Lane 1 : Human fetal liver lysate
    Lane 2 : Human small intestine lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/100000 dilution

    Predicted band size: 40 kDa
    Observed band size: 40 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Frozen sections) - Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] (ab203859)
    Immunohistochemistry (Frozen sections) - Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] (ab203859)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized Mouse liver tissue labeling Ornithine Carbamoyltransferase/OTC with ab203859 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution (green).

    Cytoplasmic staining on mouse liver is observed.

    Nuclear counterstain: DAPI (Blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] (ab203859)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] (ab203859)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Ornithine Carbamoyltransferase/OTC with ab203859 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Cytoplasmic staining on Human liver tissue is observed [PMID: 17893704].

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] (ab203859)
    Western blot - Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] (ab203859)
    All lanes : Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] (ab203859) at 1/1000 dilution

    Lane 1 : Mouse liver lysate
    Lane 2 : Rat liver lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 40 kDa
    Observed band size: 40 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 15 seconds; Lane 2: 30 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] (ab203859)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] (ab203859)

    Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue labeling Ornithine Carbamoyltransferase/OTC with ab203859 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Cytoplasmic staining on Human liver cancer tissue is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] (ab203859)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] (ab203859)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Ornithine Carbamoyltransferase/OTC with ab203859 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. 

    No staining on Human kidney is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Frozen sections) - Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] (ab203859)
    Immunohistochemistry (Frozen sections) - Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] (ab203859)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized Mouse kidney tissue labeling Ornithine Carbamoyltransferase/OTC with ab203859 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077)  at 1/1000 dilution (green).

    Weakly staining on mouse kidney.

    Nuclear counterstain: DAPI (Blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.

  • Immunoprecipitation - Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] (ab203859)
    Immunoprecipitation - Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] (ab203859)

    Ornithine Carbamoyltransferase/OTC was immunoprecipitated from 0.35 mg of Human fetal liver lysate with ab203859 at 1/40 dilution.

    Western blot was performed from the immunoprecipitate using ab203859 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: Human fetal liver lysate, 10µg (Input).

    Lane 2: ab203859 IP in Human fetal liver lysate.

    Lane 3: Rabbit IgG,monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab203859 in Human fetal liver lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] (ab203859)
    Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] (ab203859)

References (6)

Publishing research using ab203859? Please let us know so that we can cite the reference in this datasheet.

ab203859 has been referenced in 6 publications.

  • Deplazes S  et al. Intrabiliary infusion of naked DNA vectors targets periportal hepatocytes in mice. Mol Ther Methods Clin Dev 27:352-367 (2022). PubMed: 36381301
  • Zhang T  et al. Metformin Inhibits the Urea Cycle and Reduces Putrescine Generation in Colorectal Cancer Cell Lines. Molecules 26:N/A (2021). PubMed: 33915902
  • De Sabbata G  et al. Long-term correction of ornithine transcarbamylase deficiency in Spf-Ash mice with a translationally optimized AAV vector. Mol Ther Methods Clin Dev 20:169-180 (2021). PubMed: 33473356
  • Chow JPH  et al. A modified arginine-depleting enzyme NEI-01 inhibits growth of pancreatic cancer cells. PLoS One 15:e0231633 (2020). PubMed: 32353864
  • Katrekar D  et al. In vivo RNA editing of point mutations via RNA-guided adenosine deaminases. Nat Methods 16:239-242 (2019). PubMed: 30737497
  • Lee JS  et al. Urea Cycle Dysregulation Generates Clinically Relevant Genomic and Biochemical Signatures. Cell 174:1559-1570.e22 (2018). PubMed: 30100185

Customer reviews and Q&As

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725]

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: CC1 ventana
Permeabilization
No
Specification
Liver
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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Verified customer

Submitted Jul 22 2021

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