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  1. Link

    products/primary-antibodies/osteoblast-marker-antibody-zns-5-ab181413.pdf

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Signal Transduction Cytoskeleton / ECM Extracellular Matrix Structures Bone
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Anti-Osteoblast Marker antibody [ZNS-5] (ab181413)

  • Datasheet
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Key features and details

  • Mouse monoclonal [ZNS-5] to Osteoblast Marker
  • Suitable for: IHC-P, IHC-Fr
  • Reacts with: Zebrafish
  • Isotype: IgG1

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Overview

  • Product name

    Anti-Osteoblast Marker antibody [ZNS-5]
  • Description

    Mouse monoclonal [ZNS-5] to Osteoblast Marker
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-P, IHC-Frmore details
  • Species reactivity

    Reacts with: Zebrafish
    Does not react with: Human
  • Immunogen

    Tissue, cells or virus. This information is considered to be commercially sensitive.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Product was previously marketed under the MitoSciences sub-brand.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.5
    Preservative: 0.02% Sodium azide
    Constituent: 99% HEPES buffered saline
  • Concentration information loading...
  • Purity

    SDS-PAGE
  • Purification notes

    Near homogeneity as judged by SDS-PAGE. ab181413 was produced in vitro using hybridomas grown in serum-free medium and then concentrated by chemical fractionation.
  • Clonality

    Monoclonal
  • Clone number

    ZNS-5
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • Structures
    • Bone
    • Tags & Cell Markers
    • Cell Type Markers
    • Other Cell Types
    • Developmental Biology
    • Organogenesis
    • Skeletal development
    • Bone

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab181413 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Use at an assay dependent concentration.
IHC-Fr (1)
Use at an assay dependent concentration.
Notes
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.

Target

  • Relevance

    Osteoblasts are cells with single nuclei that synthesize bone. Osteoblasts are specialized, terminally differentiated products of mesenchymal stem cells. Found in the eye, nervous system (central, enteric, peripheral) and inner ear; Fin hair cell, inner ear osteoblast, retinal IPL, OPL.
  • Alternative names

    • ZNS-5 antibody
    • zns5 antibody

Protocols

  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (0)

Publishing research using ab181413? Please let us know so that we can cite the reference in this datasheet.

ab181413 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Immunohistochemistry (Frozen sections) abreview for Anti-Osteoblast Marker antibody [ZNS-5]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Sample
Zebrafish Tissue sections (Retinal inner plexiform layer, outer plexiform lay)
Specification
Retinal inner plexiform layer, outer plexiform lay
Permeabilization
Yes - triton X
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Ryan Macdonald

Verified customer

Submitted May 01 2014

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