Recombinant Anti-Osteopontin antibody [EPR21138] (ab218237)
![Immunocytochemistry/ Immunofluorescence - Anti-Osteopontin antibody [EPR21138] (ab218237)](https://www.abcam.com/ps/products/218/ab218237/Images/ab218237-489131-anti-osteopontin-antibody-epr21138-immunocytochemistry-mouse.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Osteopontin antibody [EPR21138] (ab218237)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21138] to Osteopontin
- Suitable for: ICC/IF, IHC-P
- Reacts with: Mouse
Related conjugates and formulations
Overview
-
Product name
Anti-Osteopontin antibody [EPR21138]
See all Osteopontin primary antibodies -
Description
Rabbit monoclonal [EPR21138] to Osteopontin -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- IHC-P: Mouse kidney and liver tissues. ICC/IF: RAW 264.7 cells treated with LPS (100 ng/ml) for 6 h, then add BFA (300 ng/ml) for 3 h.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR21138 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab218237 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/1000.
|
|
| IHC-P | (1) |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
ICC/IF
1/1000. |
|
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
-
Function
Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.
Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity. -
Tissue specificity
Bone. Found in plasma. -
Sequence similarities
Belongs to the osteopontin family. -
Post-translational
modificationsExtensively phosphorylated on clustered serine residues.
N- and O-glycosylated.
Phosphorylation sites are present in the extracelllular medium. -
Cellular localization
Secreted. - Information by UniProt
-
Database links
- Entrez Gene: 20750 Mouse
- SwissProt: P10923 Mouse
- Unigene: 288474 Mouse
-
Alternative names
- BNSP antibody
- Bone sialoprotein 1 antibody
- Bone sialoprotein I antibody
see all
Images
-
Immunocytochemistry/ Immunofluorescence - Anti-Osteopontin antibody [EPR21138] (ab218237)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) cells labelling Osteopontin with primary antibody anti-Osteopontin (ab218237) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) secondary antibody at 1/1000 dilution. Confocal image showing increased cytoplasmic staining in RAW 264.7 cells treated with LPS (100 ng/ml) for 6 h, then add BFA (300 ng/ml) for 3 h. Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) (ab195889) was used to counterstain tubulin at 1/200 dilution. The nuclear counter stain is DAPI (blue).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Osteopontin antibody [EPR21138] (ab218237)](https://www.abcam.com/ps/products/218/ab218237/Images/ab218237-296763-anti-osteopontin-antibody-epr21138-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Osteopontin antibody [EPR21138] (ab218237)Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Osteopontin with ab218237 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in distal tubules of mouse kidney (PMID: 17938278). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Osteopontin antibody [EPR21138] (ab218237)](https://www.abcam.com/ps/products/218/ab218237/Images/ab218237-296764-anti-osteopontin-antibody-epr21138-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Osteopontin antibody [EPR21138] (ab218237)Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling Osteopontin with ab218237 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in biliary epithelial cells of mouse liver (PMID: 16221502). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Anti-Osteopontin antibody [EPR21138] (ab218237)](https://www.abcam.com/ps/products/218/ab218237/Images/ab218237-4-benefits-of-recombinant-antibodies.png)
Anti-Osteopontin antibody [EPR21138] (ab218237)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
Certificate of Compliance
References (2)
ab218237 has been referenced in 2 publications.
- Yamashita S et al. Essential roles of oncostatin M receptor ß signaling in renal crystal formation in mice. Sci Rep 10:17150 (2020). PubMed: 33051515
- Ottaiano A et al. Cetuximab, irinotecan and fluorouracile in fiRst-line treatment of immunologically-selected advanced colorectal cancer patients: the CIFRA study protocol. BMC Cancer 19:899 (2019). PubMed: 31500586