Recombinant Anti-Oxytocin-neurophysin 1 antibody [EPR20973] (ab212193)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20973] to Oxytocin-neurophysin 1
- Suitable for: WB, IHC-P, IHC-Fr
- Reacts with: Mouse, Rat
Related conjugates and formulations
Overview
-
Product name
Anti-Oxytocin-neurophysin 1 antibody [EPR20973]
See all Oxytocin-neurophysin 1 primary antibodies -
Description
Rabbit monoclonal [EPR20973] to Oxytocin-neurophysin 1 -
Host species
Rabbit -
Specificity
Specific expression in pituitary tissue in WB and posterior lobe in IHC.
-
Tested applications
Suitable for: WB, IHC-P, IHC-Frmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: Mouse and rat pituitary tissue lysates. IHC-P: Mouse and rat hypothalamus tissues. IHC-Fr: Mouse and rat brain (Paraventricular nucleus) tissues.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol, PBS -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20973 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab212193 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000. Detects a band of approximately 12 kDa (predicted molecular weight: 12 kDa).
|
|
IHC-P |
1/8000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
|
IHC-Fr |
1/500.
Perform heat mediated antigen retrieval with sodium citrate buffer pH 6.0 before commencing with IHC staining protocol. |
Notes |
---|
WB
1/1000. Detects a band of approximately 12 kDa (predicted molecular weight: 12 kDa). |
IHC-P
1/8000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IHC-Fr
1/500. Perform heat mediated antigen retrieval with sodium citrate buffer pH 6.0 before commencing with IHC staining protocol. |
Target
-
Relevance
Neurophysin 1 specifically binds oxytocin. Oxytocin causes contraction of the smooth muscle of the uterus and of the mammary gland. Oxytocin is a posterior pituitary hormone which is synthesized as an inactive precursor in the hypothalamus along with its carrier protein neurophysin I. Together with neurophysin, it is packaged into neurosecretory vesicles and transported axonally to the nerve endings in the neurohypophysis, where it is either stored or secreted into the bloodstream. Oxytocin contracts smooth muscle during parturition and lactation. It is also involved in cognition, tolerance, adaptation and complex sexual and maternal behaviour, as well as in the regulation of water excretion and cardiovascular functions. -
Cellular localization
Secreted -
Database links
- Entrez Gene: 18429 Mouse
- Entrez Gene: 25504 Rat
- SwissProt: P35454 Mouse
-
Alternative names
- MGC126890 antibody
- MGC126892 antibody
- Neurophysin 1 antibody
see all
Images
-
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized mouse brain (paraventricular nucleus) tissue labeling Oxytocin-neurophysin 1 with ab212193 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution (green). Cytoplasmic staining on neurons located at the paraventricular nucleus (PVN) on mouse brain tissue section (PMID:24464043). The nuclear counterstain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oxytocin-neurophysin 1 antibody [EPR20973] (ab212193)
Immunohistochemical analysis of paraffin-embedded mouse hypothalamus tissue labeling Oxytocin-neurophysin 1 with ab212193 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on the neurons of mouse paraventricular nucleus (PMID: 24464043). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized rat brain (paraventricular nucleus) tissue labeling Oxytocin-neurophysin 1 with ab212193 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution (green). Cytoplasmic staining on neurons located at the paraventricular nucleus (PVN) on rat brain tissue section (PMID:24464043). The nuclear counterstain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.
-
All lanes : Anti-Oxytocin-neurophysin 1 antibody [EPR20973] (ab212193) at 1/1000 dilution
Lane 1 : Mouse pituitary tissue lysate
Lane 2 : Rat pituitary tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 12 kDa
Observed band size: 12 kDaExposure times: Lane 1: 3 seconds; Lane 2: 1 second.
Blocking/Dilution buffer: 5% NFDM/TBST.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oxytocin-neurophysin 1 antibody [EPR20973] (ab212193)
Immunohistochemical analysis of paraffin-embedded rat hypothalamus tissue labeling Oxytocin-neurophysin 1 with ab212193 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on the neurons of rat paraventricular nucleus and supraoptic nucleus (PMID: 24464043). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
Certificate of Compliance
References (9)
ab212193 has been referenced in 9 publications.
- Lei Y et al. Leptin enhances social motivation and reverses chronic unpredictable stress-induced social anhedonia during adolescence. Mol Psychiatry 27:4948-4958 (2022). PubMed: 36138127
- Fan J et al. Reinvestigating Tumor-Ventricle Relationship of Craniopharyngiomas With Predominantly Ventricular Involvement: An Endoscopic Endonasal Series Based on Histopathological Assessment. Front Oncol 11:740410 (2021). PubMed: 34926255
- Crane-Smith Z et al. A Highly Conserved Shh Enhancer Coordinates Hypothalamic and Craniofacial Development. Front Cell Dev Biol 9:595744 (2021). PubMed: 33869166
- Sarn N et al. Germline nuclear-predominant Pten murine model exhibits impaired social and perseverative behavior, microglial activation, and increased oxytocinergic activity. Mol Autism 12:41 (2021). PubMed: 34088332
- Arabacı Tamer S et al. Regular moderate exercise alleviates gastric oxidative damage in rats via the contribution of oxytocin receptors. J Physiol 598:2355-2370 (2020). PubMed: 32266969
- Zhou L et al. Colocalization of dopamine receptors in BDNF-expressing peptidergic neurons in the paraventricular nucleus of rats. J Chem Neuroanat 106:101794 (2020). PubMed: 32315740
- Liu XY et al. Effects of Intranasal Oxytocin on Pup Deprivation-Evoked Aberrant Maternal Behavior and Hypogalactia in Rat Dams and the Underlying Mechanisms. Front Neurosci 13:122 (2019). PubMed: 30863276
- Ran X et al. Distribution of D1 and D2 receptor- immunoreactive neurons in the paraventricular nucleus of the hypothalamus in the rat. J Chem Neuroanat 98:97-103 (2019). PubMed: 31018158
- Yuan XS et al. Whole-Brain Monosynaptic Afferent Projections to the Cholecystokinin Neurons of the Suprachiasmatic Nucleus. Front Neurosci 12:807 (2018). PubMed: 30455627