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RecombinantRabMAb

Recombinant Anti-Oxytocin-neurophysin 1 antibody [EPR20973] (ab212193)

Reviews (1) Submit a question References (6)
Immunohistochemistry (Frozen sections) - Anti-Oxytocin-neurophysin 1 antibody [EPR20973] (ab212193)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oxytocin-neurophysin 1 antibody [EPR20973] (ab212193)
  • Immunohistochemistry (Frozen sections) - Anti-Oxytocin-neurophysin 1 antibody [EPR20973] (ab212193)
  • Western blot - Anti-Oxytocin-neurophysin 1 antibody [EPR20973] (ab212193)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oxytocin-neurophysin 1 antibody [EPR20973] (ab212193)
  • Anti-Oxytocin-neurophysin 1 antibody [EPR20973] (ab212193)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20973] to Oxytocin-neurophysin 1
  • Suitable for: WB, IHC-P, IHC-Fr
  • Reacts with: Mouse, Rat

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-Oxytocin-neurophysin 1 antibody [EPR20973]
    See all Oxytocin-neurophysin 1 primary antibodies

  • Description

    Rabbit monoclonal [EPR20973] to Oxytocin-neurophysin 1

  • Host species

    Rabbit

  • Specificity

    Specific expression in pituitary tissue in WB and posterior lobe in IHC.

  • Tested applications

    Suitable for: WB, IHC-P, IHC-Frmore details

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse and rat pituitary tissue lysates. IHC-P: Mouse and rat hypothalamus tissues. IHC-Fr: Mouse and rat brain (Paraventricular nucleus) tissues.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab212193 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000. Detects a band of approximately 12 kDa (predicted molecular weight: 12 kDa).
IHC-P
1/8000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr
1/500.

Perform heat mediated antigen retrieval with sodium citrate buffer pH 6.0 before commencing with IHC staining protocol.
Notes
WB
1/1000. Detects a band of approximately 12 kDa (predicted molecular weight: 12 kDa).
IHC-P
1/8000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr
1/500.

Perform heat mediated antigen retrieval with sodium citrate buffer pH 6.0 before commencing with IHC staining protocol.

Target

  • Relevance

    Neurophysin 1 specifically binds oxytocin. Oxytocin causes contraction of the smooth muscle of the uterus and of the mammary gland. Oxytocin is a posterior pituitary hormone which is synthesized as an inactive precursor in the hypothalamus along with its carrier protein neurophysin I. Together with neurophysin, it is packaged into neurosecretory vesicles and transported axonally to the nerve endings in the neurohypophysis, where it is either stored or secreted into the bloodstream. Oxytocin contracts smooth muscle during parturition and lactation. It is also involved in cognition, tolerance, adaptation and complex sexual and maternal behaviour, as well as in the regulation of water excretion and cardiovascular functions.

  • Cellular localization

    Secreted

  • Database links

    • Alternative names

      • MGC126890 antibody
      • MGC126892 antibody
      • Neurophysin 1 antibody
      • Neurophysin I antibody
      • Ocytocin antibody
      • OT antibody
      • OT NPI antibody
      • OXT antibody
      • Oxytocin antibody
      • Oxytocin Neurophysin preproprotein antibody
      • Oxytocin prepro (neurophysin I) antibody
      • Oxytocin prepropeptide antibody
      • Oxytocin-neurophysin 1 antibody
      see all

    Images

    • Immunohistochemistry (Frozen sections) - Anti-Oxytocin-neurophysin 1 antibody [EPR20973] (ab212193)
      Immunohistochemistry (Frozen sections) - Anti-Oxytocin-neurophysin 1 antibody [EPR20973] (ab212193)

      Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized mouse brain (paraventricular nucleus) tissue labeling Oxytocin-neurophysin 1 with ab212193 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution (green). Cytoplasmic staining on neurons located at the paraventricular nucleus (PVN) on mouse brain tissue section (PMID:24464043). The nuclear counterstain is DAPI (blue).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oxytocin-neurophysin 1 antibody [EPR20973] (ab212193)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oxytocin-neurophysin 1 antibody [EPR20973] (ab212193)

      Immunohistochemical analysis of paraffin-embedded mouse hypothalamus tissue labeling Oxytocin-neurophysin 1 with ab212193 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on the neurons of mouse paraventricular nucleus (PMID: 24464043). Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Frozen sections) - Anti-Oxytocin-neurophysin 1 antibody [EPR20973] (ab212193)
      Immunohistochemistry (Frozen sections) - Anti-Oxytocin-neurophysin 1 antibody [EPR20973] (ab212193)

      Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized rat brain (paraventricular nucleus) tissue labeling Oxytocin-neurophysin 1 with ab212193 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution (green). Cytoplasmic staining on neurons located at the paraventricular nucleus (PVN) on rat brain tissue section (PMID:24464043). The nuclear counterstain is DAPI (blue).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

    • Western blot - Anti-Oxytocin-neurophysin 1 antibody [EPR20973] (ab212193)
      Western blot - Anti-Oxytocin-neurophysin 1 antibody [EPR20973] (ab212193)
      All lanes : Anti-Oxytocin-neurophysin 1 antibody [EPR20973] (ab212193) at 1/1000 dilution

      Lane 1 : Mouse pituitary tissue lysate
      Lane 2 : Rat pituitary tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Developed using the ECL technique.

      Predicted band size: 12 kDa
      Observed band size: 12 kDa



       

      Exposure times: Lane 1: 3 seconds; Lane 2: 1 second.

      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oxytocin-neurophysin 1 antibody [EPR20973] (ab212193)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oxytocin-neurophysin 1 antibody [EPR20973] (ab212193)

      Immunohistochemical analysis of paraffin-embedded rat hypothalamus tissue labeling Oxytocin-neurophysin 1 with ab212193 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on the neurons of rat paraventricular nucleus and supraoptic nucleus (PMID: 24464043). Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Anti-Oxytocin-neurophysin 1 antibody [EPR20973] (ab212193)
      Anti-Oxytocin-neurophysin 1 antibody [EPR20973] (ab212193)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (6)

    Publishing research using ab212193? Please let us know so that we can cite the reference in this datasheet.

    ab212193 has been referenced in 6 publications.

    • Crane-Smith Z  et al. A Highly Conserved Shh Enhancer Coordinates Hypothalamic and Craniofacial Development. Front Cell Dev Biol 9:595744 (2021). PubMed: 33869166
    • Sarn N  et al. Germline nuclear-predominant Pten murine model exhibits impaired social and perseverative behavior, microglial activation, and increased oxytocinergic activity. Mol Autism 12:41 (2021). PubMed: 34088332
    • Zhou L  et al. Colocalization of dopamine receptors in BDNF-expressing peptidergic neurons in the paraventricular nucleus of rats. J Chem Neuroanat 106:101794 (2020). PubMed: 32315740
    • Liu XY  et al. Effects of Intranasal Oxytocin on Pup Deprivation-Evoked Aberrant Maternal Behavior and Hypogalactia in Rat Dams and the Underlying Mechanisms. Front Neurosci 13:122 (2019). PubMed: 30863276
    • Ran X  et al. Distribution of D1 and D2 receptor- immunoreactive neurons in the paraventricular nucleus of the hypothalamus in the rat. J Chem Neuroanat 98:97-103 (2019). PubMed: 31018158
    • Yuan XS  et al. Whole-Brain Monosynaptic Afferent Projections to the Cholecystokinin Neurons of the Suprachiasmatic Nucleus. Front Neurosci 12:807 (2018). PubMed: 30455627

    Customer reviews and Q&As

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    Immunocytochemistry/ Immunofluorescence abreview for Anti-Oxytocin-neurophysin 1 antibody [EPR20973]

     Good
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (hiPSC derived hypothalamic neurons)
    Permeabilization
    Yes - 0.1%Triton X-100 in blocking solution
    Specification
    hiPSC derived hypothalamic neurons
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Sandra Horschitz

    Verified customer

    Submitted Apr 13 2022

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