Anti-P2Y12 antibody (ab183066)
Key features and details
- Rabbit polyclonal to P2Y12
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-P2Y12 antibody
See all P2Y12 primary antibodies -
Description
Rabbit polyclonal to P2Y12 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human P2Y12 aa 1-100 (N terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. Genbank: NP_073625
Database link: Q9H244 -
Positive control
- Human 293T and HeLa whole cell lysate (ab150035); Human lung cancer tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS
PBS without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab183066 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/400 - 1/2000. Predicted molecular weight: 39 kDa.
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| IHC-P |
1/25 - 1/100.
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| Notes |
|---|
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WB
1/400 - 1/2000. Predicted molecular weight: 39 kDa. |
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IHC-P
1/25 - 1/100. |
Target
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Function
Receptor for ADP and ATP coupled to G-proteins that inhibit the adenylyl cyclase second messenger system. Not activated by UDP and UTP. Involved in platelets aggregation. -
Tissue specificity
Highly expressed in the platelets, lower levels in the brain. Lowest levels in the lung, appendix, pituitary and adrenal gland. Expressed in the spinal cord and in the fetal brain. -
Involvement in disease
Defects in P2RY12 are the cause of P2RY12 deficiency (P2RY12D) [MIM:609821]. It is a condition characterized by severe impairment of platelet response to ADP and abnormal bleeding marked by excessive posttraumatic and postsurgical blood loss. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 64805 Human
- Omim: 600515 Human
- SwissProt: Q9H244 Human
- Unigene: 591281 Human
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Alternative names
- ADP glucose receptor antibody
- ADP-glucose receptor antibody
- ADPG R antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2Y12 antibody (ab183066)
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue labeling P2Y12 with ab183066 at 1/25 dilution, in the presence (right image) or absence (left image) of immunizing peptide.
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Western blot - Anti-P2Y12 antibody (ab183066)All lanes : Anti-P2Y12 antibody (ab183066) at 1/400 dilution
Lane 1 : 293T cell lysate
Lane 2 : HeLa cell lysate
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : Goat anti Rabbit IgG - H&L (HRP) at 1/5000 dilution
Developed using the ECL technique.
Predicted band size: 39 kDa
Exposure time: 30 seconds
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab183066 has been referenced in 3 publications.
- Zhang Q et al. Platelet miR-107 Participates in Clopidogrel Resistance after PCI Treatment by Regulating P2Y12. Acta Haematol 145:46-53 (2022). PubMed: 34474410
- Ogaki A et al. Replacement of Mouse Microglia With Human Induced Pluripotent Stem Cell (hiPSC)-Derived Microglia in Mouse Organotypic Slice Cultures. Front Cell Neurosci 16:918442 (2022). PubMed: 35910250
- Satti HH et al. Antiplatelet activity of astaxanthin in control- and high cholesterol-fed rats mediated by down-regulation of P2Y12, inhibition of NF-?B, and increasing intracellular levels of cAMP. Platelets N/A:1-10 (2020). PubMed: 32379559